ISKki2

  • Family IS1595
  • Group IS1016
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
LN869922 ND Kingella kingae
Kingella kingae KKKWG1
DNA section
IS Length : 740 bp

Ends


IR Length : 22

IRL : GGGGCTGTCCTAGATAACTAGGGAAATTCAAATTAAGTTAGAATTATCCC
IRR : GGGGCTGTCCTAGATAACTAGGATAAACTCGATTTTACTAATTGTTTTAA

Insertion site


Left flankDirect repeatRight flankDR Length
ATTCAACATTAAAAAGAAATTGATTTTTTTACCTCCATCA0
TTCGTTTCATAAAAAGATTAAAAAATAATGCACCATTTAT0
TATTTCGGGAAATTAAAAAAAATTTTTAACATTCGAGCAC0
GTGTATAGTGAATTAAATTTAGATTAGTACAG8
CAAAAATGATAAAATATCTTTATGACTAGAAA8

DNA sequence

GGGGCTGTCCTAGATAACTAGGGAAATTCAAATTAAGTTAGAATTATCCCTATGCGAAAATGTCGTCTAAGTCAGTATAAACAAAACAAACTCATTGAGC
TATTTGTCGCAGGTGTAACCGCAAGAACAGCAGCAGAGTTAGTAGGTGTTAATAAAAATACCGCAGCCTATTATTTTCATCGTTTACGATTATTTATCTA
TCAAAACAGTCCACATTTAGAAATGTTTGATGACGAAGTAGAAGCAGATGAAAGTTATTTCGGTGGACAACGAAAAGGTAAACGCGGTCGCGGGGCTGCT
GGAAAAGTCGCTGTATTCGGGCTCTTGAAGCGAAATGGTAAAGTTTACACTGTTACCGTACCGAATACTAAAACAGCCACTTTGTTACCCATTATTCGTG
AACAAGTTAAACCTGACAGCATTGTTTATACGGATTGTTATCGTAGTTATGATGTATTAGATGTGAGCGACAAAGCCATTTTAGCTTCGCTGAAACTTCG
TTTTCGTATCAATCACAGCACACATTTTGCTGAACGACAAAACCATATTAACGGAATTGAGAACTTTTGGAACCAAGCAAAACGTCATTTACGCAAGTTT
AACGGCATTCCCAAAGAGCATTTTGAGCTGTATTTAAAGGAGTGCGAATGGCGTTTTAACAACAGTGAGATAAAAGTTCAAATTTCTATTTTAAAACAAT
TAGTAAAATCGAGTTTATCCTAGTTATCTAGGACAGCCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
672 bp223 aa52723+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKCRLSQYKQNKLIELFVAGVTARTAAELVGVNKNTAAYYFHRLRLFIYQNSPHLEMFDDEVEADESYFGGQRKGKRGRGAAGKVAVFGLLKRNGKVYT
VTVPNTKTATLLPIIREQVKPDSIVYTDCYRSYDVLDVSDKAILASLKLRFRINHSTHFAERQNHINGIENFWNQAKRHLRKFNGIPKEHFELYLKECEW
RFNNSEIKVQISILKQLVKSSLS

 

Blast result :
Comments
ISKki2 is 88% aa similar to ISHpa1.
References
1] ISfinder annottaion (2017)
2] Bidet,Philippe. (2015) Direct GenBank submission.