ISAs2

  • Family IS30
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) ISAS2
Accession numberTranspositionOriginHost
L27157 Y Aeromonas salmonicida
Aeromonas salmonicida A449-ceg4
DNA section
IS Length : 1084 bp

Ends


IR Length : 24/29

IRL : CGGGAATTCAGATAATAAGTGCGACACTCATGGATGAATGGCGAGAGGTG
IRR : CGCGAATTCAACTTCGAAGTGCGACACTCGCCTATGCCGCCTGACGGTAC

Insertion site


Left flankDirect repeatRight flankDR Length
NNNNNNNNNNCTGNNNNNNNNNN3
AACTCTAAACACCTACATTTAAA3
ATGCTAACCATATAACTGGAAGG3
CCTGTGAACGAAGTGACTCAAGC3
TAAGAGGCAGACTGACTATCACA3
TGGTAGTGATCTTAACCATCCCC3
CTACCTGGTTCAAGTTACAT0

DNA sequence

CGGGAATTCAGATAATAAGTGCGACACTCATGGATGAATGGCGAGAGGTGGCCAACTGCTGATTGGTGGTACCCTTCTGCTCGCCAAAGTAAAAGCAGTA
TCACCATGAGCTATCAGCAGTTGACCGAGGGGCAACGATACCAGTTATCGGTGCTTCGCGCACAGGGGATGTCCATCCTTGCCACCGCACGCGCCATTGG
GGTTCATCGCTCCACCCTCTACCGGGAGCTGCGTCGTAACGCCGGGCCGCAGGGCTACCAGCCAGACAATGCGCATCAGCACGCCACCCACCGACGGGCC
AGTGCGGCCAAATCTCGGCTATCTGCCGATGTCATCCAGTTTCTCGAGCTGACACTCGCCTGGTGGTGGAGCCCGGAGCAGATAAGCGCGGTGGGCAAGC
AGATAGGCCTCATGGTGAGTCACGAGTGGATATACCGCCATGTGGCCGCCGACAAAGCCCGGGGTGGCCAGTTGTACCGCCATCTGCGCCAGGGCCACAA
GCGCTACCGCAAGGGGGCGAGCAGCCTGCGTTCTCCCATCAAGGAAGCCCGCTCTATTGATGAACGGCCGGCTATCGTCGATAGCCGGGAGCGGCTCGGT
GATTGGGAGGCGGACACGGTGCTGGGCAAGCAAGGCACAGGTGCACTGGTCACCCTGGTGGAGCGCAAGAGCCGGCTCTATCTGGTCAAGCGGGTCGCGA
ACAAACAGGCTGGGGTGGTGCGGGATGCGATAATCGAGATGTTAACGCCCTACATCGAGCAGGTGCATACCATCACCTTTGACAACGGCGGCGAGTTTGC
CGAACACAAGGCGATAGAGGAAGCGCTGGGTGCCGAGACTTACTTTGCGCACCCTTACTCTTCGTGGGAGCGCGGTCTGAACGAGAACAGCAATGGTCTG
TTGAGGCAGTTTATCCCAAAGGGAACAGACCTGAGAGAGGTCACGGACGAGGATGTCAGGAGAGCGGAGCAGTGGCTCAACCTACGACCTCGGAAATGTC
TCGGATTCAGGCAGCCTGTCAAGGTATTCGAAGAGTACCGTCAGGCGGCATAGGCGAGTGTCGCACTTCGAAGTTGAATTCGCG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
948 bp315 aa1061053+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSYQQLTEGQRYQLSVLRAQGMSILATARAIGVHRSTLYRELRRNAGPQGYQPDNAHQHATHRRASAAKSRLSADVIQFLELTLAWWWSPEQISAVGKQI
GLMVSHEWIYRHVAADKARGGQLYRHLRQGHKRYRKGASSLRSPIKEARSIDERPAIVDSRERLGDWEADTVLGKQGTGALVTLVERKSRLYLVKRVANK
QAGVVRDAIIEMLTPYIEQVHTITFDNGGEFAEHKAIEEALGAETYFAHPYSSWERGLNENSNGLLRQFIPKGTDLREVTDEDVRRAEQWLNLRPRKCLG
FRQPVKVFEEYRQAA

 

Blast result :
Comments
Aeromonas salmonicida is a fish pathogen whose major virulence factor is a tetragonal paracrystalline surface protein array (A-layer), encoded by the vapA gene. ISAs2 has been found as an insertion in the vapA gene, after growing the strain at 30¡ C (Is transposition triggered by high temperature ?). This is the first example of IS-dependent mutagenesis of an S-layer. Two proteins (38 and 39 kDa), presumably corresponding to the putative ISAs2 transposase, have been observed in an Escherichia coli S30 transcription-translation system.
File updated the March 03 2015 with the information provided by F. Pfeiffer.
References
1] Gustafson, C.E., Chu, S. and Trust, T.J. (1994) J. Mol. Biol. 237, 452-463.
2] Zhao, S., and Hill, C.W. (1995) J. Bacteriol. 177, 1393-1398.