ISAs2
- Family IS30
- Group
Isoform Synonym(s) ISAS2
Accession number | Transposition | Origin | Host |
---|---|---|---|
L27157 | Y | Aeromonas salmonicida | Aeromonas salmonicida A449-ceg4 |
DNA section
IS Length : 1084 bp
Ends
IR Length : 24/29
IRL : CGGGAATTCAGATAATAAGTGCGACACTCATGGATGAATGGCGAGAGGTG
IRR : CGCGAATTCAACTTCGAAGTGCGACACTCGCCTATGCCGCCTGACGGTAC
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
NNNNNNNNNN | CTG | NNNNNNNNNN | 3 |
AACTCTAAAC | ACC | TACATTTAAA | 3 |
ATGCTAACCA | TAT | AACTGGAAGG | 3 |
CCTGTGAACG | AAG | TGACTCAAGC | 3 |
TAAGAGGCAG | ACT | GACTATCACA | 3 |
TGGTAGTGAT | CTT | AACCATCCCC | 3 |
CTACCTGGTT | CAAGTTACAT | 0 |
DNA sequence
CGGGAATTCAGATAATAAGTGCGACACTCATGGATGAATGGCGAGAGGTGGCCAACTGCTGATTGGTGGTACCCTTCTGCTCGCCAAAGTAAAAGCAGTA
TCACCATGAGCTATCAGCAGTTGACCGAGGGGCAACGATACCAGTTATCGGTGCTTCGCGCACAGGGGATGTCCATCCTTGCCACCGCACGCGCCATTGG
GGTTCATCGCTCCACCCTCTACCGGGAGCTGCGTCGTAACGCCGGGCCGCAGGGCTACCAGCCAGACAATGCGCATCAGCACGCCACCCACCGACGGGCC
AGTGCGGCCAAATCTCGGCTATCTGCCGATGTCATCCAGTTTCTCGAGCTGACACTCGCCTGGTGGTGGAGCCCGGAGCAGATAAGCGCGGTGGGCAAGC
AGATAGGCCTCATGGTGAGTCACGAGTGGATATACCGCCATGTGGCCGCCGACAAAGCCCGGGGTGGCCAGTTGTACCGCCATCTGCGCCAGGGCCACAA
GCGCTACCGCAAGGGGGCGAGCAGCCTGCGTTCTCCCATCAAGGAAGCCCGCTCTATTGATGAACGGCCGGCTATCGTCGATAGCCGGGAGCGGCTCGGT
GATTGGGAGGCGGACACGGTGCTGGGCAAGCAAGGCACAGGTGCACTGGTCACCCTGGTGGAGCGCAAGAGCCGGCTCTATCTGGTCAAGCGGGTCGCGA
ACAAACAGGCTGGGGTGGTGCGGGATGCGATAATCGAGATGTTAACGCCCTACATCGAGCAGGTGCATACCATCACCTTTGACAACGGCGGCGAGTTTGC
CGAACACAAGGCGATAGAGGAAGCGCTGGGTGCCGAGACTTACTTTGCGCACCCTTACTCTTCGTGGGAGCGCGGTCTGAACGAGAACAGCAATGGTCTG
TTGAGGCAGTTTATCCCAAAGGGAACAGACCTGAGAGAGGTCACGGACGAGGATGTCAGGAGAGCGGAGCAGTGGCTCAACCTACGACCTCGGAAATGTC
TCGGATTCAGGCAGCCTGTCAAGGTATTCGAAGAGTACCGTCAGGCGGCATAGGCGAGTGTCGCACTTCGAAGTTGAATTCGCG
TCACCATGAGCTATCAGCAGTTGACCGAGGGGCAACGATACCAGTTATCGGTGCTTCGCGCACAGGGGATGTCCATCCTTGCCACCGCACGCGCCATTGG
GGTTCATCGCTCCACCCTCTACCGGGAGCTGCGTCGTAACGCCGGGCCGCAGGGCTACCAGCCAGACAATGCGCATCAGCACGCCACCCACCGACGGGCC
AGTGCGGCCAAATCTCGGCTATCTGCCGATGTCATCCAGTTTCTCGAGCTGACACTCGCCTGGTGGTGGAGCCCGGAGCAGATAAGCGCGGTGGGCAAGC
AGATAGGCCTCATGGTGAGTCACGAGTGGATATACCGCCATGTGGCCGCCGACAAAGCCCGGGGTGGCCAGTTGTACCGCCATCTGCGCCAGGGCCACAA
GCGCTACCGCAAGGGGGCGAGCAGCCTGCGTTCTCCCATCAAGGAAGCCCGCTCTATTGATGAACGGCCGGCTATCGTCGATAGCCGGGAGCGGCTCGGT
GATTGGGAGGCGGACACGGTGCTGGGCAAGCAAGGCACAGGTGCACTGGTCACCCTGGTGGAGCGCAAGAGCCGGCTCTATCTGGTCAAGCGGGTCGCGA
ACAAACAGGCTGGGGTGGTGCGGGATGCGATAATCGAGATGTTAACGCCCTACATCGAGCAGGTGCATACCATCACCTTTGACAACGGCGGCGAGTTTGC
CGAACACAAGGCGATAGAGGAAGCGCTGGGTGCCGAGACTTACTTTGCGCACCCTTACTCTTCGTGGGAGCGCGGTCTGAACGAGAACAGCAATGGTCTG
TTGAGGCAGTTTATCCCAAAGGGAACAGACCTGAGAGAGGTCACGGACGAGGATGTCAGGAGAGCGGAGCAGTGGCTCAACCTACGACCTCGGAAATGTC
TCGGATTCAGGCAGCCTGTCAAGGTATTCGAAGAGTACCGTCAGGCGGCATAGGCGAGTGTCGCACTTCGAAGTTGAATTCGCG
Protein section
ORF number : 1
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
948 bp | 315 aa | 106 | 1053 | + | No |
Chemistry : DDE
ORF sequence :
MSYQQLTEGQRYQLSVLRAQGMSILATARAIGVHRSTLYRELRRNAGPQGYQPDNAHQHATHRRASAAKSRLSADVIQFLELTLAWWWSPEQISAVGKQI
GLMVSHEWIYRHVAADKARGGQLYRHLRQGHKRYRKGASSLRSPIKEARSIDERPAIVDSRERLGDWEADTVLGKQGTGALVTLVERKSRLYLVKRVANK
QAGVVRDAIIEMLTPYIEQVHTITFDNGGEFAEHKAIEEALGAETYFAHPYSSWERGLNENSNGLLRQFIPKGTDLREVTDEDVRRAEQWLNLRPRKCLG
FRQPVKVFEEYRQAA
GLMVSHEWIYRHVAADKARGGQLYRHLRQGHKRYRKGASSLRSPIKEARSIDERPAIVDSRERLGDWEADTVLGKQGTGALVTLVERKSRLYLVKRVANK
QAGVVRDAIIEMLTPYIEQVHTITFDNGGEFAEHKAIEEALGAETYFAHPYSSWERGLNENSNGLLRQFIPKGTDLREVTDEDVRRAEQWLNLRPRKCLG
FRQPVKVFEEYRQAA
Blast result :
Comments
Aeromonas salmonicida is a fish pathogen whose major virulence factor is a tetragonal paracrystalline surface protein array (A-layer), encoded by the vapA gene. ISAs2 has been found as an insertion in the vapA gene, after growing the strain at 30¡ C (Is transposition triggered by high temperature ?). This is the first example of IS-dependent mutagenesis of an S-layer. Two proteins (38 and 39 kDa), presumably corresponding to the putative ISAs2 transposase, have been observed in an Escherichia coli S30 transcription-translation system.
File updated the March 03 2015 with the information provided by F. Pfeiffer.
File updated the March 03 2015 with the information provided by F. Pfeiffer.
References
1] Gustafson, C.E., Chu, S. and Trust, T.J. (1994) J. Mol. Biol. 237, 452-463.
2] Zhao, S., and Hill, C.W. (1995) J. Bacteriol. 177, 1393-1398.
2] Zhao, S., and Hill, C.W. (1995) J. Bacteriol. 177, 1393-1398.