ISfinder

ISKor1

Family IS1595
Group IS1016

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
ACJW02000003	ND	Kingella oralis	Kingella oralis ATCC 51147

DNA section

IS Length : 713 bp

Ends

IR Length : 19

IRL : GGGGCTGTACTAGATAAGCAGTCATGTTAGACTGCAAAAATGAAGATAAC
IRR : GGGGCTGTACTAGATAAGCCCTAAATACCACACCATCTGCGCAGCACTTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACCTAGATAGATTTTCATAA		ATAATAAGATGAGCGTCAAA	0
TTAGAAAACGCA	AAAATTAT	ACGCTTTTGGGC	8
GCCTCAAATACA	AATACAAA	AGGGCGTATTGC	8
CAAGCGCGGTAA	AAACATTT	TGCCCGCCATGA	8

DNA sequence

GGGGCTGTACTAGATAAGCAGTCATGTTAGACTGCAAAAATGAAGATAACCCATTGTAAACTAAAAAAGAGTATACGAAAAAAGTTGCTCCAATTTTTTG
TACTTGAAGTAACCGCCCGTTCGGCTGCTGATTTGTTGGGCATTCACCCCAATTCAGCAGCACTGTTCTACCGCAAAATCCGCGAAGTCATCAGCTACTA
TCTTTCATTGGAAGCCAATACAGTTTTTGATGGTACGGTTGAGCTAGATGAGAGTTACTTCGGCGGACATCGCAAAGGCAAACGCGGACGCGGAGCAGCA
GGAAAAGTAGCTGTCTTTGGTATTCTTAAGCGTGGTGGTAAAGTTTATACCGTAGTAGTGAAGAATGCCAAAAGAGAAACCTTAATGCCTGTTATCACAA
AGAAAATCATGCCTGATAGTATTGTTTATACCGATTGTTTGAGCAGCTATGATGTGTTGGATGTAAGTGGTTTTACCCACCACCGTATTAACCACAGCAA
ACTGTTTGCTGACAAACAAAACCACATCAACGGCATTGAAAATTTTTGGAATCAGGCGAAACGTGTTCTGCGTAAATACAATGGAATTGACCGTAAATCT
TTCCCATTATTCTTGAAAGAATGCGAGTTTCGTTTTAACTTTGGCACACCGTCTGAACAGCTTAAAGTGCTGCGCAGATGGTGTGGTATTTAGGGCTTAT
CTAGTACAGCCCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
654 bp	217 aa	40	693	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKITHCKLKKSIRKKLLQFFVLEVTARSAADLLGIHPNSAALFYRKIREVISYYLSLEANTVFDGTVELDESYFGGHRKGKRGRGAAGKVAVFGILKRGG
KVYTVVVKNAKRETLMPVITKKIMPDSIVYTDCLSSYDVLDVSGFTHHRINHSKLFADKQNHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFN
FGTPSEQLKVLRRWCGI

Blast result :

Comments

ISKor1 is 92% aa similar to IS1016C2.

References

1] ISfinder annotation (2017)
2] Weinstock,G., Sodergren,E., Clifton,S., Fulton,L., Fulton,B., Courtney,L., Fronick,C., Harrison,M., Strong,C., Farmer,C., Delahaunty,K., Markovic,C., Hall,O., Minx,P., Tomlinson,C., Mitreva,M., Nelson,J., Hou,S., Wollam,A., Pepin,K.H., Johnson,M., Bhonagiri,V., Nash,W.E., Warren,W., Chinwalla,A., Mardis,E.R. and Wilson,R.K. (2009) Direct GenBank submission.