ISAzvi1

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s) IS101
Accession numberTranspositionOriginHost
AF322366 Y Azotobacter vinelandii
Azotobacter vinelandii OP
Azotobacter vinelandii UW
DNA section
IS Length : 1026 bp

Ends


IR Length : 25

IRL : GGCTATGTCTGAGTTAGCTGTTGCAGCAGGCTAAGCTGATCATGGCGTCT
IRR : GGCTATGTCTGAGTTAGCTGTTGCATGGCTACTCGCCCCAGCGCCTCCCT

Insertion site


Left flankDirect repeatRight flankDR Length
TCAAGAGGCTTTCATCAAAGCCTATCGT8
GCTCAAGAGGCTTTCATCAAAGCCTATCGTGC8
ATTATCGGGGAGTTAGGCAAAGACCTTGGAGG8

DNA sequence

GGCTATGTCTGAGTTAGCTGTTGCAGCAGGCTAAGCTGATCATGGCGTCTCTGCATGCGGGAGGTGGATCATGAAAGCGAGTCAGTTCACGCGATGGATC
GCTCAGCTCTCCAACCTCAGCCCGGAACAGCGTGAGCAGCTGAAGGCATGCCTGTCGGCACCAGGCAGCCTTCCGCAGGAGATGATCGCCACGCCCAGCA
GTTGCCCGCACTGTCAGTCCAGCGAGTTACAGCCGTGGGGCTCCAGTGGCGGCCTGCCACGGTATCGCTGCAAGTTTTGCGGCAAAACCAGCAACCCATT
GACGGGTACACCGATGGCGCGCCTGCGAAAGCGCCATCTCTGGCAAGGCTATGCCGAGGCGTTGACCCAGAGCCTGACGGTACGCAGGGCGGCGAAACAC
TGTGGCGTCAGTAAGAACACAGCCTTCCTGTGGCGACACCGGTTCCTGACCCAGATCGCCGATCACCAGGCTCAGCATGAGTCCGGGATTGTCGAGGCCG
ATGAAACCTTCTTCCTGGAGTCTTTCAAGGGACAGCGAGACTTGCCGCGCCCACCTCGCAGGCGCGGAGGCAGCGCGAAGCAACGCGGGTTGTCTGCCGA
GCAGATTCCTGTCCTGGTGGTGAGAGATCGCAGCGGCCAGCATGCCGACTTTCAGCTGAAGAAGCTCGATGCCGTGCATGTCAGGGAGCGACTACGCCCC
CTGATCGATGCTGATGCGATTCTTTGCTCTGACAGTGCCGCCGTCTATGCCCACTTTGCCAAGGCAGAAGGCATCACCCATCAGCCGGTCAATCCGAGCC
AGAAGCGCAGGGTCGATGGGCCTTTTCACATCCAGAACGTGAACGCCTATGACAGCCGACTGAAGAGCTGGATGATTCCCTTTCATGGGGTGGCCACCAA
GTATCTGACCCATTACCTGGGATGGCGCCGCCTGCTCGAACGCTACAAGACGCAGCTCAATCCATTGATTTGCTTGAGGGAGGCGCTGGGGCGAGTAGCC
ATGCAACAGCTAACTCAGACATAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
954 bp317 aa711024+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKASQFTRWIAQLSNLSPEQREQLKACLSAPGSLPQEMIATPSSCPHCQSSELQPWGSSGGLPRYRCKFCGKTSNPLTGTPMARLRKRHLWQGYAEALTQ
SLTVRRAAKHCGVSKNTAFLWRHRFLTQIADHQAQHESGIVEADETFFLESFKGQRDLPRPPRRRGGSAKQRGLSAEQIPVLVVRDRSGQHADFQLKKLD
AVHVRERLRPLIDADAILCSDSAAVYAHFAKAEGITHQPVNPSQKRRVDGPFHIQNVNAYDSRLKSWMIPFHGVATKYLTHYLGWRRLLERYKTQLNPLI
CLREALGRVAMQQLTQT

 

Blast result :
Comments
ISAzvi1 is 47% aa similar to ISH4.
The sequence inserted in the database correspond to the complete sequence found in the sequenced genome (CP005095). The original sequence described in AF322366 exhibits a non expected frameshift and could be inactive.
References
1] Page,W.J., Tindale,A., Chandra,M. and Kwon,E.(2001) Microbiology 147 (Pt 2), 483-490
Meakins,D., Tindale,A.E. and Page,W.J (2001) Direct submission GenBank.
2] Noar,J.D. and Bruno-Barcena,J.M. (2013) Genome Announc 1 (3)