ISAcba1

  • Family IS1595
  • Group ISSod11
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
MKSW01000042 ND Actinobacteria bacterium
Actinobacteria bacterium 69-20
DNA section
IS Length : 3517 bp

Ends


IR Length : 24/27

IRL : ATTGGGTATCCGCTTGAGTCCAGTAGGTTTGAGTGGTGATCGAGCGGCTG
IRR : AGTGGGTATCTGCTTGAGTCCAGTCGGCCGATCTTCGACGGAACTACCTC

Insertion site


Left flankDirect repeatRight flankDR Length
GAGCACTCGTACAGTTGTTCGATGGTCT8

DNA sequence

ATTGGGTATCCGCTTGAGTCCAGTAGGTTTGAGTGGTGATCGAGCGGCTGCCGGTGGCCGGCGTGGATTACCCGCGCACGCATCAGCAGCTGTCCGCGTG
GTTCTCGGACGAGGACGCGTGCCTGGAGTACCTGGCCCGCTTGCGCTGGCCAGACGGATTCCGCTGCCCCTCGTGCGGTGGGGATGCCGCCTGGCGCACC
GCGCGGCGCGCGTTGTGGATGTGCGCAGGGTGCGGGCGGCAGACGTCGGTCACGGCCGGCACGATCTTCCACCGCTCGCACACCCCGTTGACGACGTGGT
TCGCGGCGATCTGGTTCGTGTGCGCGTCGAAGAACGGGGTCTCGGCGAGCACGTTGCAGCAGGTCCTCGGGTTCGGCTCGTACGAGACCGCGTGGGCGTG
GCTGCACAAGCTACGCCGGGTCATGGTTCGCCCCGATCGTGACCTGCTCGGCGGGGTCGTCGAGCTGGACGAGACCCTCGTCGGCGGGCGCGCCAAGCCC
GCCTCCAGCGTGGTGATCACCAAGGTCCCGGTCATGGTCGCGGTCGAGACCCCGGCCGGGCACCAGCTCGGACGCATTCGCCTGGCCCGCGCCGACGCCC
GTAACTCGGGCCAGCTGATCGACTTCGCCCACCAGGTCATCGCCCCTGGCACGTTGATTCGCACCGACGGTGCCCGCAACCTGCGCCGGCTCGCCGAGCA
GGGCTACACCCACGAGTACAAGGTCATGCTGACCTCACCGGACCCGGCCCATGAGTACCTGCCTGGCGTGCACCTGGTCGCTTCCCTGCTCAAACGCTGG
GTCGCCGGCACGCTGCACCACGGCATCTCCCGCCGCCAGCTCGACTACTACCTCGACGAGTTCACCTTCCGGTTCAACCGGCGCAGCTCACGCTCCCGCG
GCCTGCTGTTCTACCGGCTCCTGCAACAAGCCGTCGCCACCGACCCGCACCCATTGGCCGCACTGGTCGGCGGCACCGACCAAGACCGAGGTGGCGGCGC
GTCGTAGCAGCGTCTATCGGGCCATGGCCCGCTGTGGACTCTGCACGCTGGTCACGGTGCTTGTGGTGTGCAGGTTCGTGGCCCGTCCGGCGCGCACGCC
GTTGGCGATGACGACGACCTCGGCCAGCTCATGGACCAGGACGACCGCGGCCAGCCCAAGCACTCCGGCGACTGCCAGCGGGATCAGGATCGCGATCAGG
GTGAGCGACAGGCCGACGTTCTGCAGCATGATGCGCCGGGCCCGTCGGGCGTGGGCGAATGCCTGAGGCAGGGCGCGCAGGTCCGTGCCCATCAGGGCGA
CGTCCGCGGTCTCCAGTGCGACGTCCGAGCCCATGGCGCCCATCGCGATGCCGAGGTCGGCGGTGGCCAGGGCGGGGGCGTCGTTGACGCCGTCGCCGAC
CATTGCTGTGGGTGCCTTCGTACGCAGGGCGGCGACCAGCGTGGACTTGTCCTCTGGACGCAGCTCGGCGTGGACGTCGGCGATGCCGGCCTGGGCGGCC
ACGGCGTAAGCGGTGGCGGTGTTGTCGCCGGTAAGCATGGTGACGTTGTAGTCGTGGCGGTGCAGCTCGGCGACGACCGCGCGCGCTTCGGGTCGCAGCT
CGTCTCGGACGGCGATCGCGCCGGTCACCGTCCCGTCGTCCTCGACCAGGACGGCCGTCGCCCCGGCTTCCTGCATTCCCTGGACCTGGGCGGCCAGGTC
CGCGGCGGTGATCCACCCGGGGCGGCCCAGCCGCACGGCCCGCCCGTCGACGGTGCCGGTCAGGCCGGCGCCGGGGACCGCCTGGACATCAGCGGCGTCG
GCCACGGTGTCGACCGCGGTGAGGATCGCCCGGGCCAACGGGTGCTCGCTGCGGGCCTCCAAGGCGCCCGCCACGGCGAGGACCTGCTCGCGGGTGTGGC
CCGCGGTGGTGGCGACGGCGACGACGGTGGGGGCGTTGGCTGTCAGGGTGCCGGTCTTGTCCAGGGCGATCATGCGGATCGCGCCGAGCGCCTCCAGGGC
GGCGCCACCCTTGATCAGCACGCCGAATCGGCTGGCGGCCCCAATGGCGGAGACGACCGAGACCGGCACGGAGATGGCCAACGCGCACGGGGACGCGGCT
ACCAGCACGACCAGCGCCCGTTGGATCCATAGTCCCGGGTCCCCGAGCAGGGAGCCGATCACTGCGATGAGGGTGGCGACGATCATGATGGCGGGCACGA
GCGGCTTGGCGACCCGGTCGGCGAGCCGCTGGGTGGTGCCCTTGCGCGACTGCTCGGCCTCGACGATGTGCACGATGCGGGCCAGGGAGTTGTCTTCCGA
AGTAGTGGTGACCTCGACCTCGAGGGCTCCGGTGCCGTTGATGGATCCCGCGTACACCTGGGTGCCGGGTCCGACCTCGACGGGGATCGATTCTCCGGTG
ATGGCCGAGACGTCGAGGGTGGTGCGGCCGGTGCGGATGGTGCCGTCGGTGGCGAGCCGTTCCCCCGGCTTGACGAGCATCGTGTCGCCCACAGCGAGAT
CGTCCGGGTCGATGACCTGCTCGATTCCCGCGCGTCGGACCGTGGCCGTGTCGGGGACCAGGGAGAGGAGGGCCCTCAGCCCACCGCGGGTCTTGGCCAG
GGAGTACTCCTCCAGGCCCTCGCTCAGGGAGTACAAGAACGCGAGGGTGGCGGCCTCGGCGAACTCACCGAGGATCACTGCGCCGACGGCGGCGATCGTC
ATCAAGGTGCCGACGCCGATCTTGCCCCGGGCCAGCTTCTGCAGGGTCTCGGGCACGAAGGTGCTCGCCCCGACCAGCAGGGCGGCGATGTTCAAGCCGA
GCGCCCAACCGTGCAGGCCGCCCAGTCCGAGCAGGAAGGCAACCAGCAGGAGCGTGCCCGCGACGAGACCGGCGCGGATCTCGCTGACACCCCAGATGCT
CGTGACGTCCTTATCGTCGTCATCGTCGATCTCATCGGCGGTGGTCTGATCGGCGCCGCGGTCGTCGGTCATCGGGTGCTCTCCTCGTGGGTTGATGTGT
TCGCGGGCATCGAGGTCTGGCTGCCCGTCCGGCCAAGTCCGTAAGTGGGGCACAGCTCGACCGCGTGCCCGGTCGCGTCCAGCAACAGCTCGGCCGCGGC
GAGGACCGCAAGCAGCTCCGGGGCCGTTGTCAGGAAGAACATCGACGCCCGACCCTGCGGGCGGGAGTCGACCAGGCCACAGTCCTTCAGGCACGCCAGG
TGTGCGCTCACCGTGCTCTGCGCCAGTCCGGTGTGCTCGGTGAGATCCCGCACTCGATGCTCACCGTGCGTCAGATGCTGCAGGACGGCCAGGCGCGTCG
GGTCGGAGAGCGAGTGGAACAGCGCCGCCGCCGGGGCCAGCACACCGCCCGCGGGATCGCCGGGTTCCCCGTCGACAACCCGATCCGGTCGTATCGCCAT
GTGACGATGATAGCGGCAGGTCTGGATGGGTCCTGCCTGGCTCGCGCGCCGCCGGAGCACAAGACACGAGGTAGTTCCGTCGAAGATCGGCCGACTGGAC
TCAAGCAGATACCCACT
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
960 bp319 aa481007+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPVAGVDYPRTHQQLSAWFSDEDACLEYLARLRWPDGFRCPSCGGDAAWRTARRALWMCAGCGRQTSVTAGTIFHRSHTPLTTWFAAIWFVCASKNGVSA
STLQQVLGFGSYETAWAWLHKLRRVMVRPDRDLLGGVVELDETLVGGRAKPASSVVITKVPVMVAVETPAGHQLGRIRLARADARNSGQLIDFAHQVIAP
GTLIRTDGARNLRRLAEQGYTHEYKVMLTSPDPAHEYLPGVHLVASLLKRWVAGTLHHGISRRQLDYYLDEFTFRFNRRSSRSRGLLFYRLLQQAVATDP
HPLAALVGGTDQDRGGGAS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1959 bp652 aa29721014-No
ORF function : Passenger Gene
Annotation : Cadmium-translocating P-type ATPaseDescription :

ORF sequence :

MTDDRGADQTTADEIDDDDDKDVTSIWGVSEIRAGLVAGTLLLVAFLLGLGGLHGWALGLNIAALLVGASTFVPETLQKLARGKIGVGTLMTIAAVGAVI
LGEFAEAATLAFLYSLSEGLEEYSLAKTRGGLRALLSLVPDTATVRRAGIEQVIDPDDLAVGDTMLVKPGERLATDGTIRTGRTTLDVSAITGESIPVEV
GPGTQVYAGSINGTGALEVEVTTTSEDNSLARIVHIVEAEQSRKGTTQRLADRVAKPLVPAIMIVATLIAVIGSLLGDPGLWIQRALVVLVAASPCALAI
SVPVSVVSAIGAASRFGVLIKGGAALEALGAIRMIALDKTGTLTANAPTVVAVATTAGHTREQVLAVAGALEARSEHPLARAILTAVDTVADAADVQAVP
GAGLTGTVDGRAVRLGRPGWITAADLAAQVQGMQEAGATAVLVEDDGTVTGAIAVRDELRPEARAVVAELHRHDYNVTMLTGDNTATAYAVAAQAGIADV
HAELRPEDKSTLVAALRTKAPTAMVGDGVNDAPALATADLGIAMGAMGSDVALETADVALMGTDLRALPQAFAHARRARRIMLQNVGLSLTLIAILIPLA
VAGVLGLAAVVLVHELAEVVVIANGVRAGRATNLHTTSTVTSVQSPQRAMAR

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
432 bp143 aa34002969-No
ORF function : Passenger Gene
Annotation : Transcriptional regulatorDescription : Transcriptional Regulator factor

ORF sequence :

MAIRPDRVVDGEPGDPAGGVLAPAAALFHSLSDPTRLAVLQHLTHGEHRVRDLTEHTGLAQSTVSAHLACLKDCGLVDSRPQGRASMFFLTTAPELLAVL
AAAELLLDATGHAVELCPTYGLGRTGSQTSMPANTSTHEESTR

 

Blast result :
Comments
ISAcba1 is 72% aa similar to ISMva1.
References
1] ISfinder annotation (2017)
2] Kantor,R.S., Huddy,R.J., Iyer,R., Thomas,B.C., Brown,C.T., Anantharaman,K., Tringe,S., Hettich,R.L., Harrison,S.T. and Banfield,J.F. (2016) Direct GenBank submission.