ISKne1

  • Family IS1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CCNJ00000000 ND Kingella negevensis
Kingella negevensis Sch538T
DNA section
IS Length : 807 bp

Ends


IR Length : 28/29

IRL : GGTGGTATCCTGAAAAGTGTGCTGTAAAAAATAAGCAATAAAAAAGCAGC
IRR : GGTGGTGTCCTGAAAAGTGTGCTGTAAAACTAAACAATGCCAAAGTGAAT

Insertion site


Left flankDirect repeatRight flankDR Length
ATCATAACTAGGCAAAATTGAAATAACTTTCATCTGCTTC0

DNA sequence

GGTGGTATCCTGAAAAGTGTGCTGTAAAAAATAAGCAATAAAAAAGCAGCGAAAACAGAGTATATTTGAGTGTGTGAAAACAGAAATAACTCTAATTTGT
CCTCGCTGCCAAGGACAAAATATAAAGAAAAATGGCTACTCTGGCAATCGTAAACAAAAGTATTTTTGTAAAGATTGCTGCCGTAATTTTATTGGCGACC
ATAACCTTACCTATAAGGGTTGTCATTCCAAAGCTGATGAACAGGTTTGGAGAATGACCGTTAGAGGTTGTGGCATTCGCGATATTGCAGCAATTACGGG
TTACAGCAAAGACAAAGTTCAGGCTGCCTTAAAACGCCATGAGTTTGAGCCATTTCCTAAACAAAAACATTACTCTACACTTGAAATAGACGAGTTTTGG
ACATTTGTCGGTAACAAGTCTAATAAAGTGTGGCTAGTCTATGCCTATCACAGAGAAAGTGGCGAAATTGTCGCTTACGTTTGGGGTAAGCGCGATTTAG
CAACAGCGCGAGCACTCAAACGGCGTTTGAAAGAGTTGCGTGTAACCTATGACAGAATTGCGACCGACGACTGGGCTGCATTTTTAAATGTCTTTTCAAA
TGAAGAATGGCATCTAGTTGGTAAGCAACACACAGTTGGCATTGAGGGTAATAACTGTCGTTTACGGCACAAAGTAAGGCGAGCGTTTAGAAAAACGTGT
TGCTTTTCTAAAAGTATGTTTTATCATAAGAAAGTTTTTGATTTGGCTTTCTTTGATATTCACTTTGGCATTGTTTAGTTTTACAGCACACTTTTCAGGA
CACCACC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
705 bp234 aa74778+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKTEITLICPRCQGQNIKKNGYSGNRKQKYFCKDCCRNFIGDHNLTYKGCHSKADEQVWRMTVRGCGIRDIAAITGYSKDKVQAALKRHEFEPFPKQKHY
STLEIDEFWTFVGNKSNKVWLVYAYHRESGEIVAYVWGKRDLATARALKRRLKELRVTYDRIATDDWAAFLNVFSNEEWHLVGKQHTVGIEGNNCRLRHK
VRRAFRKTCCFSKSMFYHKKVFDLAFFDIHFGIV

 

Blast result :
Comments
ISKne1 is 70% aa similar to ISPa14.
References
1] El Houmami N (2017) Direct submission.