ISKpn41

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
JN420336 ND Klebsiella pneumoniae
Klebsiella pneumoniae pNDM-MAR
DNA section
IS Length : 1426 bp

Ends


Left end : GTATTTAGTCACAAAAACAATCTGTACATGTAATAAATAGGCAGAATTCCGACTTCTGTTTATTTCCTGCACCCGTTGACTCCGTGATGTATATATGGTT II struct. : No
Right end : CCGTTAAGCGAGGAACCGGGCAATGCGGTAACGCGTTGCTACCCTACAAAGGGAATCCCCTTCCTGATTCACGTAGTGAGAAGGTGGGGGAGGATGTCAA II struct. : No

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GAGCGCATTCGTTCAGGTAGCTGAACATTTCCAGTCAA

DNA sequence

GTATTTAGTCACAAAAACAATCTGTACATGTAATAAATAGGCAGAATTCCGACTTCTGTTTATTTCCTGCACCCGTTGACTCCGTGATGTATATATGGTT
ATTTATACAGGCTACATCAATACACACTGAATGCAAAATGCTAATCCAGAAAGCGTATAAATACAGACTCAACCCGACAGATGCACAGGCGCAACGACTG
CGTCAGATGTGCGGCTGCGCCCGTTTTGTCTGGAATTACGCCCTGGCAGAGACGCAGCGTATTCTTGAGGCCGGACAAAAACTCCCTTCCGCCTTTGAGC
TGAATAAAATGCTGACAGGATGGAAGAAATTACCTGAGCTGGCGTTTTTATCTGATGCCTACACCGACAACCTTCAGCAGAAACTGAAAGACCTGCACGG
GGCATGGAAACGGTGCTTTGATAAAAAGCTGGCAGCGAAAGCGCCCGTGTTTAAACATAAAAGCCACGGCAATGACTCTGTTCGTTTCGTCAATTTTGAC
AAATACTGCCAGCTTGAGTACAGACGCGTTAAGTTACCCTCGGGGCTGGGCTGGGTGACATTCCGGCAGTCCCGCGCCGTTGTGGGTAAAATCAAATACG
CAACGGTCAGCCAGCACGCTGGCGGCTGGTATATCTCGTTTCAGGTAGAAATGGAGGTCGAACAGCCCGCACACCCGTCAACGTCTGCTGTCGGCCTGGA
TGCCGGTATCAGCAAGCTGGTAACGCTGTCAGACGGCACGGTGTTTGAACCTGTCAACAGTTTTAAACAGAACCAAAGCAGACTGGCAAGGTTACAGCGC
CAGCTTGCCCGTAAAGTAAAATTCAGTGCGAACTGGAAGAAGCAGAAAGCAAGAATTTCCCGCTTACATTCGCATATCGCCAACATCCGCCGCGATTACC
TACACAAAGTCACAACAACAGTCAGCAAAAACCACGCGATGATCGTTATTGAAGATTTGAAGGTTTCCAACATGTCGAAATCGGCAGCGGGTACGGTAGA
CGAGCCCGGGCGCAACGTTGCAGCAAAATCAGGTCTTAACCGGGCGATACTGGATCAGGGCTGGTACGAAATGCGCCGCCAGCTCGAATATAAGCAGCGA
TGGCGCGGCGGTGAGGTGCTGGCAGTAAATCCGGCATATACCAGCCAGAAATGCGCGCGTTGCGGTCATACAGTAAAGGAAAATCGTCAATCGCAGGCAG
TATTTGTCTGCGTGGCGTGTGGTTACGAAGCGAACGCCGACATAAACGGCGCTCAAAATATTCTCGCGGCAGGACATGCCGTGTTGTCCGGGATTAACCC
CGGACGCGCCCGTAAGGCGGCGTAAGCCGTTAAGCGAGGAACCGGGCAATGCGGTAACGCGTTGCTACCCTACAAAGGGAATCCCCTTCCTGATTCACGT
AGTGAGAAGGTGGGGGAGGATGTCAA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1239 bp412 aa871325+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MYIWLFIQATSIHTECKMLIQKAYKYRLNPTDAQAQRLRQMCGCARFVWNYALAETQRILEAGQKLPSAFELNKMLTGWKKLPELAFLSDAYTDNLQQKL
KDLHGAWKRCFDKKLAAKAPVFKHKSHGNDSVRFVNFDKYCQLEYRRVKLPSGLGWVTFRQSRAVVGKIKYATVSQHAGGWYISFQVEMEVEQPAHPSTS
AVGLDAGISKLVTLSDGTVFEPVNSFKQNQSRLARLQRQLARKVKFSANWKKQKARISRLHSHIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSA
AGTVDEPGRNVAAKSGLNRAILDQGWYEMRRQLEYKQRWRGGEVLAVNPAYTSQKCARCGHTVKENRQSQAVFVCVACGYEANADINGAQNILAAGHAVL
SGINPGRARKAA

 

Blast result :
Comments
ISKpn41 is 85% aa similar to ISEc41.
The left end is done by the BLAST comparison with other sequences. There is no evidence for a canonical cleavage site.
References
1] Dongsheng Zhou (2017) Direct submission.
2] Carattoli,A. (2012) J. Antimicrob. Chemother. 67 (7), 1645-1650.