ISKpn43

  • Family IS110
  • Group IS1111
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP024839 ND Klebsiella pneumoniae
Klebsiella pneumoniae CRKP-1215 pCRKP-1215_1
DNA section
IS Length : 1374 bp

Ends


IR Length : 12

IRL : TAATGGAATGGTCGCCTCCCATCTCTTGTGCCACAGTGGGACGATAAATC
IRR : ----TAGATGGTCGCCTCCTGTTTGCCAAGCTAAATATGTTGTGGTGGCT

Comments : The IR of this IS are sub-terminal with 7nt separating the IRL from the left-hand and and 4nt separating the IRR from the right-hand end, a characteristic of members of the IS1111 group (Partridge and Hall, 2003).

Insertion site


Left flankDirect repeatRight flankDR Length
TATCTAAACGGCTTTGTTGAATAAATCGAACTT0

DNA sequence

TAATGGAATGGTCGCCTCCCATCTCTTGTGCCACAGTGGGACGATAAATCGTCACTCATTGAACGGAGCGCTGCTATCATGAACGTTAAAACTATTGGAA
TCGATTTGGCAAAAAACGTTTTCCAGATCCATGGGGTTGACGAGCACGGAAAACGGTTGTTCAACAAACAACTCAGACGGGCACAAATGGCCTCCTTTTT
TGCCAACATCCCACCCTGTTTGATCGGCATGGAGGCCTGTGCATCTGCTCATTTCTGGGCCAATAAACTGATATCGATGGGCCATAATGTCAAACTGATG
GCCCCTCAGTTCGTCAAACCCTATGTTAAAACCAATAAGCATGATGCTGCAGACGCTGAAGCTATTTGTGAAGCCGTCACTCGACCTAACATGCGGTTCG
TGCCGGTCAAAACCGCTGAGCAGCAAGCCGTATTGGCACTTCACCGGAGTCGTCAGAGCTTCATCAAACAGCGAACCGCACAAGCCAATCAAATCAGGGG
GTTATTGGCCGAATTTGGCATTGTCGTCCCCCGAGGTATCCAGCAGCTACAGCGACGATTACCTGAGCTCGTGGAAGATGCGGATAACCCGTTACCCGTC
CTGTTTCGTACACAGCTGAGTCTACTACAGCACCACATGGCGTACCTGTTCGATGTCATCGCTACACTCGACAAGCAGATTGAGCAGTGCTATCGGCAAA
ATGCTCTCTGCCAGCGTATCGGCAAGATCCCTGGTATTGGCCCTGTTACCTCCAGCGCGCTGATTGCGACCATTGGTAAAGCCAACAATTTCGAGAATGG
CCGACAACTGGCTGCCTGGCTCGGATTGGTTCCACGTCAGCACTCCAGTGGGGGTAAACAAGTCCTGCTCGGGATAAGCAAGCGAGGTGATACCTATTTG
AGGACCTTGCTTATCCATGGTGCCAGGGCGGTATTGCAGTCGGCCAAACATAAACAGGATGCCGTATCGAGCTGGGCTAACCAGCTAATGGCGCGCCGGA
ATAACAACATTGCCTCGGTAGCATTGGCTAACAAGAATGCGCGGACTGTGTGGGCGCTACTGGCCAAAGAGCGGGAGTATTGTGCACCAATAATAAGCGC
TTAAGTTGCTTAATCAGTAAGACAGAAACAACACCACCGATTGCCCAGGCAAGCATGAAGTGATGGCAAGACAGGTCAGACCGCGATGGGGAAAACCCGG
TTTATTCAAGGCTCCTGAAAGAGCGCTTTGTTGATAGAGGCCTCCATCAGCGTATTCCATCAGGGACAGAGGTATTTTACATCACCTCGGCAAAGTCCGG
ATCTATGGCTGCAATCGATGACCAGTAAAGCCACCACAACATATTTAGCTTGGCAAACAGGAGGCGACCATCTA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1026 bp341 aa791104+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MNVKTIGIDLAKNVFQIHGVDEHGKRLFNKQLRRAQMASFFANIPPCLIGMEACASAHFWANKLISMGHNVKLMAPQFVKPYVKTNKHDAADAEAICEAV
TRPNMRFVPVKTAEQQAVLALHRSRQSFIKQRTAQANQIRGLLAEFGIVVPRGIQQLQRRLPELVEDADNPLPVLFRTQLSLLQHHMAYLFDVIATLDKQ
IEQCYRQNALCQRIGKIPGIGPVTSSALIATIGKANNFENGRQLAAWLGLVPRQHSSGGKQVLLGISKRGDTYLRTLLIHGARAVLQSAKHKQDAVSSWA
NQLMARRNNNIASVALANKNARTVWALLAKEREYCAPIISA

 

Blast result :
Comments
ISKpn43 is 79% aa similar to ISAve1.
ISKpn43 is inserted into a partial copy of IS903B (IS5 family member).
References
1] Shujie Wang (2018) Direct submission.
2] Yoon,E.-J., Kang,D.Y. and Jeong,S.H. (2017) Direct GenBank submission.