ISKpn42

  • Family IS110
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP025642 ND Klebsiella pneumoniae
Klebsiella pneumoniae LS355 pKP7
DNA section
IS Length : 1161 bp

Ends


Left end : TGTATCTTGAAGACTGATTAAAATTAATACGATTAGATTTGGCAAAACGTATGCAGCCCGATGCCTTCCAGGGTACAAGCCCGTATATCAGCGACTGGCG
Right end : GCGTAAGCTGCTTACCATCCTGAACGCAATGCTCAGAAAGAACGAAGAATGGGATGAGTCGTACCACCACGTTGCTCCATAATTTTTACCTTCAAGACAG

Insertion site


Left flankDirect repeatRight flankDR Length
GTGAGACTGCCTTC/GCTGATATTGCTT5

DNA sequence

TGTATCTTGAAGACTGATTAAAATTAATACGATTAGATTTGGCAAAACGTATGCAGCCCGATGCCTTCCAGGGTACAAGCCCGTATATCAGCGACTGGCG
CATACGCCGAGCCACTCATCTGAAACCCGAACAGTTGTCCGTATTCCATAACGTATTTGCAAGGAGGGGTAAAAATGAGTCAGCCAAATCCACTTTGTGT
GGGCATCGATGTTTCCAAAGCCACACTGGACATCGCTGCCAGCAGTGATGTTGCTCAGTTTACAGTCAGCAATGACTCTGATGGCTTTAACGCGATTATT
ACCGGACTGAGACGGCACTCCGTTGCTCTGGTTCTGATGGAGGCCACTGGTGGGCTGGAAGTTGCGGTGGCCTGTTCGCTTCAGGCCGAAGGTTTTGAGG
TTGCCGTGGTCAATCCCAGGCAGGCCCGTGACTTCGCCCGTGCAATGGGATATCTTGCGAAGACCGACCGAATTGATGCCAGAGTCCTCACGCAGATGGC
TGAGGTTATTAATCGTCACCCTGAGCGGAAACGCTTTATCAGGGCACTGCCGGATGCTGAACGTCAGGCCCTTGCTGCGATGGTGGTTCGCAGACGCCAG
TTGATTGCGATGTTGGTGGCTGAGCGTAACCGACTGCATCCATTCCATCCGCAGAACAAGAAAAGCATTAATACCATCATTAAAGCGCTGGAAGATGAGC
TTGTCAGGCTCGAGAAAGAAATGGACAGTCACATCCGTAACCACTTTAAAGAGATTGCTGAGCGCCTGAGCAGTATCAAAGGTGTAGGCACCATGACCGT
TTCCGCGATGTTGGCGGAGATTCCGGAACTGGGAACGCTCTCCCGACGCGAAATCAGTGCGCTTATAGGTGTTGCTCCTGTCAACCGGGACTCAGGGACT
ATGCGTGGCCGGCGAACCATTTTTGGCGGCAGGGCTGGCGTAAGATCAGCACTGTACATGGCTGCGCTTGTGGCAACCCGCTTTAATCCGGTGATAAAAA
CGTTTTATGTGCGCCTGCTTGCAGCCGGAAAGGCCAAAAAAGTGGCGCTGGTAGCCTGTATGCGTAAGCTGCTTACCATCCTGAACGCAATGCTCAGAAA
GAACGAAGAATGGGATGAGTCGTACCACCACGTTGCTCCATAATTTTTACCTTCAAGACAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
969 bp322 aa1751143+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MSQPNPLCVGIDVSKATLDIAASSDVAQFTVSNDSDGFNAIITGLRRHSVALVLMEATGGLEVAVACSLQAEGFEVAVVNPRQARDFARAMGYLAKTDRI
DARVLTQMAEVINRHPERKRFIRALPDAERQALAAMVVRRRQLIAMLVAERNRLHPFHPQNKKSINTIIKALEDELVRLEKEMDSHIRNHFKEIAERLSS
IKGVGTMTVSAMLAEIPELGTLSRREISALIGVAPVNRDSGTMRGRRTIFGGRAGVRSALYMAALVATRFNPVIKTFYVRLLAAGKAKKVALVACMRKLL
TILNAMLRKNEEWDESYHHVAP

 

Blast result :
Comments
ISKpn42 is 81% aa similar to ISSm3_aa1.
ISKpn42 is inserted into a copy of ISEc36 (IS3 family member). The insertion generate a 5 bp imperfect duplication
References
1] Fei Chen (2017) Direct submission.
2] Tang,Y., Jin,J., Fu,P. and Jiang,X. (2018) Direct GenBank submission.