ISAs10

  • Family IS30
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
KY555069 ND Aeromonas salmonicida
Aeromonas salmonicida subsp. salmonicida 01-B526 plasmid pAsa5
DNA section
IS Length : 1221 bp

Ends


IR Length : 23/26

IRL : TGTAGATTCAATTGATCAACGCAACAACCATGTGAAAACATGCAGCTGCG
IRR : TGTAGATTCAACTTGTCAACGCAACACTCTTTTCAATAACTTCTTTTGGC

Insertion site


Left flankDirect repeatRight flankDR Length
AAAGGAGGCCGCGACGTATTTG2

DNA sequence

TGTAGATTCAATTGATCAACGCAACAACCATGTGAAAACATGCAGCTGCGGAGGATTGTTGAATGAAACGAACATTTACAGCAAAGGAAAAAGCGTTTGT
TTTTGATCTGTGGAAGCGCGGTACTGGCTTCAGTGATATAGCAAAAATCCTGGATTAAAAACCCGGTACAATTTTCACCATGTTGAGAGACACGGGAGGC
ATCAAACCGACCGAGCGTCGCTGAGCAGTAGCTCACCTGACGCTATCCGAACGTGAGGAGATTCGAGCCGGTCTTTCTGCAAAGATGAGTATCCGTGATA
TAGCGCGGGCCCTTAACCGCTCTCCATCAACAATATCTCGGGAAGTTCAACGCAACCGTGGCAGACGCTATTACAAAGCTGTTGATGCTAATAATCGCGC
TAACCGCATGGCTAAAAGACCTAAAGCATGCCTTCTCGAACGGAATTCGCGATTGCGAGAACTGGTGCTGGAAAAACTCGAGTTGAAATGGTCTCCAGAG
CAGATATCGGGGGGGTTCAAAAGAACGATGCCTCGCCAGAAAGCGATGCAGATCTCTGCCGAAACGATCTATAAAACGCTGTATTTTCGTAGCCGGAGTG
CGCTACACCATCTGCTTGTCAAGCATCTACGTCGTTCTCACAGCCTGCGTCATGGGAAACGCCATACCCGCAAGGGAGAGAGGGGCACCATCAATATAGT
GAACGGTATTTCAATCCATGAGCGCTCCAGACACATCGATAATCGACGAACTTTTGGTCACTGGGAAGGCGACTTGGTTTCCGGCACTAACAACTCCCAC
ATTGCCACGCTAGTAGATAGAAAGTCGCGCTACACCATCATTCTCAAGCTAAGTGGAAAAGATGCTGGTTCTGTGAATCAAGCAATTACAGAAAAATTTA
AGACGTTGCCGCGTAAGCTCAGACAGTCGCTGACATGGGATCGCGGCATGGAACTGGCCAAGCATCTGGAGTTTACTGCAAACGCTGGGGTGAAGGTTTA
CTTCTGTGATCCCCAGAGTCCCTGGCAACGGGGGACGAATGAAAACACCAATGGGCTGATCCGACAATACTTCCCCAAAAAGACATGTCTTGCCCAATAT
TCCCAGCAAGAGCTGGATACGGTTGCAGCGCAACTAAACAGCAGACCGAGGAAAATCCTGAAATTTAAAACGCCAAAAGAAGTTATTGAAAAGAGTGTTG
CGTTGACAAGTTGAATCTACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1152 bp382 aa631214+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKRTFTAKEKAFVFDLWKRGTGFSDIAKILD*KPGTIFTMLRDTGGIKPTERR*AVAHLTLSEREEIRAGLSAKMSIRDIARALNRSPSTISREVQRNRG
RRYYKAVDANNRANRMAKRPKACLLERNSRLRELVLEKLELKWSPEQISGGFKRTMPRQKAMQISAETIYKTLYFRSRSALHHLLVKHLRRSHSLRHGKR
HTRKGERGTINIVNGISIHERSRHIDNRRTFGHWEGDLVSGTNNSHIATLVDRKSRYTIILKLSGKDAGSVNQAITEKFKTLPRKLRQSLTWDRGMELAK
HLEFTANAGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYSQQELDTVAAQLNSRPRKILKFKTPKEVIEKSVALT

 

Blast result :
Comments
ISAs10 is 98% aa similar to ISAhy3.
There are multiple copies … the transposase could be active despite the two STOP codons in frame.
References
1] Tanaka,K.H., Vincent,A.T., Emond-Rheault,J.-G., Adamczuk,M., Frenette,M. and Charette,S.J. (2017) Direct GenBank submission.