ISPye55

  • Family IS1182
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP024423 ND Paracoccus yeei
Paracoccus yeei
Paracoccus yeei TT13
DNA section
IS Length : 1600 bp

Ends


IR Length : 17/18

IRL : GAGCCTGTGTGGAAACTCTGCTTTATGTTAGACTCGGACATTGTGACGGG
IRR : TAGCCTGTGTGGAAACTCGCCGCATCGGGAGGGGGTGGTTCTTCGGACTG

Insertion site


Left flankDirect repeatRight flankDR Length
ccctgccttgAGCCcaaacccgcc4
cgacgctttgAGCCcattgcggac4
gcctccatacAGCCcaaacccgaa4

DNA sequence

GAGCCTGTGTGGAAACTCTGCTTTATGTTAGACTCGGACATTGTGACGGGGGATCGGCATGTCGGGCTTCATTGAGGGCGTTGACCGCGCGCAGACGGTC
CTGTTTCCAGATCGGCTCGACGATTGGATTGACGAGGATCATCTGGTTCGCGTGGTTGATCTGTTTGTTGACGAGTTGGATCTGCCGGCTCTTGGCTTTG
CGCGTTCGGCACCAGCACGGACCGGTCGGCCCGGCTATCATCCTGCGATTCTGCTCAAGTTGTTCATCTATGGCTATCTGAACCGGATCCCGTCCAGCCG
CAGGCTGGAGCGGGAGGCTAGTCGAAATGTCGAACTGATGTGGCTGACCGGCCGCCTGGTTCCTGACCACAAGACCATCGCCGACTTCCGCCGCGACAAC
GGACCCGCCATTCGCCGAGTCTGCGCGCAGTTCGTCGAGCTCTGCCGCCGCATCGGCACCCTCAAGGGCGATTGCGTAGCCATCGACGGGAGCAAGTTCA
GAGCCGTCAACAACCGTGACCGCAATTTCACCAAGGGCAAGATCGCCAGCCGCATCGCGCATCTGGACGCCGACGTCGAGCGCTACATTCAGGAGATGGT
CCGGACCGACCGCCAGGAAGAAGGCGAGGCACGGGCTGACAAGGTCGCCCACCTCGGCCGCCGATATGGACGCATCAAGCAGGAGATCGAGCGGCTGAAG
GCAATGGACAAAGCCTTGGCCGACGCACCTGACGGTCAGATCTCGTTGACTGATCCTGATGCCCGCGCCATGGCCACCAGCGCCCGCAACAGCGGAATGG
TCGGCTACAATGTGCAGACGGCGGTGGACGCAGAAACTCACCTGATCGTGGCCCATGACGTCACCAACCAGGTGCATGATCGTGATCTTCTGGCACCAAT
GGCCACAGCGGCCAAAGAGGTATTGGCCCGCAACGAGCTGCATGTTCTGGCTGACAAGGGCTACTTCAGCAGCCGCGAGATCCTGACCTGCTTTGACGCA
GCCATCACCACAACCGTGCCGCGCCCCGAGACCTCGGGCAACCGCGGCAAAGGCATGTATGTGAAGGCCGACTTCGCTTACGAACCAGACGCGGATGTTT
ATCGCTGCCCTGCTGGCGAGGCGCTGAGCTATCGCTATACTGCCGAGGAAGAAGGCCTCCAGTTGCGGCGCTACTGGACCGGCGAATGTGGCAGCTGCCC
CATCAAGTCCCGCTGCACGACCGGCAAAGAACGCCGGATTACGCGATGGGAGCATGAGCACCTGATCGAAGAAGCGCAGATCAGGTTGCGGGGTGCATCA
GAGCCGATGACGGCGCGCCGATCCACCGTCGAGCACCCGTTCGGCACGATCAAGGCATGGATGGGGGCGACCCATTTTCTGACCAGGCGGCTGCCCAACG
TGAAGACGGAAATGGCGCTGAACGTGCTGGCCTACAACATCAAGCGAATGGTGGCCTTGATCGGCATCCGGAACTTGATGACCGCGATGCGGGCATGAAA
AGGAAGGAAAGCTCGCCTCTTTTCTGGCCGCGCTCGTGAAGTGCTGCGCCCAGTCCGAAGAACCACCCCCTCCCGATGCGGCGAGTTTCCACACAGGCTA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1440 bp479 aa591498+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSGFIEGVDRAQTVLFPDRLDDWIDEDHLVRVVDLFVDELDLPALGFARSAPARTGRPGYHPAILLKLFIYGYLNRIPSSRRLEREASRNVELMWLTGRL
VPDHKTIADFRRDNGPAIRRVCAQFVELCRRIGTLKGDCVAIDGSKFRAVNNRDRNFTKGKIASRIAHLDADVERYIQEMVRTDRQEEGEARADKVAHLG
RRYGRIKQEIERLKAMDKALADAPDGQISLTDPDARAMATSARNSGMVGYNVQTAVDAETHLIVAHDVTNQVHDRDLLAPMATAAKEVLARNELHVLADK
GYFSSREILTCFDAAITTTVPRPETSGNRGKGMYVKADFAYEPDADVYRCPAGEALSYRYTAEEEGLQLRRYWTGECGSCPIKSRCTTGKERRITRWEHE
HLIEEAQIRLRGASEPMTARRSTVEHPFGTIKAWMGATHFLTRRLPNVKTEMALNVLAYNIKRMVALIGIRNLMTAMRA

 

Blast result :
Comments
ISPye55 is 89% aa similar to ISPse1.
ISPye55 was identified by in silico sequence analysis of Paracoccus yeei strain TT13 (3 copies present in the genome).
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Lim, J. Y., Hwang, I., Ganzorig, M., Pokhriyal, S., Singh, R., & Lee, K. (2018). Complete Genome Sequence of Paracoccus yeei TT13, Isolated from Human Skin. Genome Announcements, 6(3), e01514–17.