ISHsal1

  • Family ISH3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
- ND Halobacterium salinarum
Halobacterium salinarum DSM 3754
DNA section
IS Length : 1389 bp

Ends


IR Length : 22/28

IRL : CAGTACATCACAAAGCCGCTTAGTTAGAACGTCTGCTTGCTGAAGGTGTG
IRR : CAGTACCTCACAAAGCATCGCCAGTTAGCCTGACCTGAGCCATCTGTTCT

Insertion site


Left flankDirect repeatRight flankDR Length
GAACGAGGATGGATTCTAGTCTCAGTGAACATCC0
TACTCGGTGGCAAGTTTGGTGCGGTCATC5
AGATTTATACACAATCTGGCTTGAAGACG5
CAGGGAGAATACATTATGGTCGTCGTGAT0
TCCTCTGTCTGGATACTCGGTTCGGCGGC0

DNA sequence

CAGTACATCACAAAGCCGCTTAGTTAGAACGTCTGCTTGCTGAAGGTGTGTCTACGACCCAGCAAGCAGACAGTGAGATTCACGAGGACCAGCTTCTTAA
CTTTCTCGTCAACACCCTTGAGGAGGAAGTTCCGCTTTCCTTAGCCAATAACGCTGAAATCACTACTGAGGGCATCTATGAGGTCCTCGTCGGCGCTTGC
GCCGACGGGACCTCTGTCTCTACGCTCTGTGCGTCGAGTCAGAACGGCCCCGCTGCCAACACGATCCTCTACCATCTCCGGACGAAGTTCGAGCCCAAAC
GGCTCGAACGAGTCGCTAACACGCTTCTTCGACGAGATATCGTCGAACTGCTTCCTGAGCAGGTGGAGGTCTGCGCAGACCTCCACCTGCGGCCCTACTA
CGGTGATGAAGACGACACGGAGAACCTCTATCACTCGGAAGCCAAGCGTGGAACCACCGCATTCCACGCCTACGCCACACTCTACGCGCGTGTGAAGAAC
AAACGATATACGCTGGCGGTGCGCCGTCTCGAAGACGGCGACACCGCCAGCAGTGTCCTCGCTGAGTTTCTTGGTGTACTCGACGGCCTTGACGCCGATG
TCAAGGCCGTCTACCTTGATCGCGGATTCTACGACAGCAAGTGTCTCACGCTGTTGCAGGCGCACAACTACGCCTACGTGGTCCCGATCATTCAGTGGGG
GAAGACGATTCAGCAGGAACTTGCGGAAGGGTGGAGTCGCGTCATTCAACACGATCTGACGGGGAAACTCGACGGTCACAGCTGGACCGTCGAGTTTCCC
GTCTACATCGACTGTACGTACCTGAACGGACGGTACGACGACCACGGTGTGGCGCGTCACGGCTACGCCGCTGACGCGCCGTTTATCGAGACACCACGCC
AGGCTCGATACCATTACTCGAAACGCTTCGGTATCGAGTCAAGCTATCGCTTGTCCGAGCAAGCGATAGCGACGACAACGACGCGAAACGCGACGGTGAG
ACTTCTGTACGTTGTGGTGAGTCTGCTCTTGCAGAATGCGTGGCGGTATCTGCACTACGAATACGTGGCGACGCCGCGCCGAGGCGGGCGTCGCCTCTGG
TGGTGGCCGTACAAGGAGTTCGTGAATATGGTTCGGCGGGCTGCGTGGACGGCCCTCGCGGTGCGTCGGGCCGTCCCCGCGAATCGGCCACCTGACGACC
GGTTCCACCGGTAGTCACCGACCGAGCAAGCTCCCTTCAGGAGTGGCAACGCTGTCGCGTCGGCGGCTGACTGCCGCCGACAGCGACAGCTCCCTCTTCG
ATCAGCGTTGCTCGACCGTTACTGACGACTCATCACAACAGAACAGATGGCTCAGGTCAGGCTAACTGGCGATGCTTTGTGAGGTACTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1167 bp388 aa481214+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSTTQQADSEIHEDQLLNFLVNTLEEEVPLSLANNAEITTEGIYEVLVGACADGTSVSTLCASSQNGPAANTILYHLRTKFEPKRLERVANTLLRRDIVE
LLPEQVEVCADLHLRPYYGDEDDTENLYHSEAKRGTTAFHAYATLYARVKNKRYTLAVRRLEDGDTASSVLAEFLGVLDGLDADVKAVYLDRGFYDSKCL
TLLQAHNYAYVVPIIQWGKTIQQELAEGWSRVIQHDLTGKLDGHSWTVEFPVYIDCTYLNGRYDDHGVARHGYAADAPFIETPRQARYHYSKRFGIESSY
RLSEQAIATTTTRNATVRLLYVVVSLLLQNAWRYLHYEYVATPRRGGRRLWWWPYKEFVNMVRRAAWTALAVRRAVPANRPPDDRFHR

 

Blast result :
Comments
ISHsal1 is 96% aa similar to ISH3C.
References
1] Pfeiffer F., Marchfelder A., Habermann BH., Dyall-Smith M. (2018) Direct submission.