ISHsal2

  • Family ISH3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Halobacterium salinarum
Halobacterium salinarum DSM 3754
DNA section
IS Length : 1399 bp

Ends


IR Length : 16

IRL : CAGTACCTCACAAAGCTGGTTAGTGAGAACGTCTGCTTGCTGATGGTGTG
IRR : CAGTACCTCACAAAGCATCCTCGGCTAGCTGTTTCTGCGGCCTGAGTTCT

Insertion site


Left flankDirect repeatRight flankDR Length
CGCCACAAGGTCATACTCGCGGGCGTAGT5
AACAGTTCAGTGACGATACAGACCTAGAC5

DNA sequence

CAGTACCTCACAAAGCTGGTTAGTGAGAACGTCTGCTTGCTGATGGTGTGTCTAGAACCAAGCAAGCAGACAGTGAGATCCACGAAGACCAGCTCCTTAA
CTTCCTCGTCAACCAGCTTGACGAGGAAGTTTCTCTCGGCCTTGCGAACAACGCTGAAATCGAACCTGAGGACATCTACGAGGTCCTCGTCGGCGCTTGC
GCCGACGGGACCTCGGTCTCGACGCTCTGTGAGAGAAGCGAAGATTCACCGCCTGCAAACACGGTCCTGTACCATCTGCGGACGAAGTTCGAGCTCGACT
GGCTCGAACGAGTCGCTAACACGCTCCTTCGGCGAGATATCGTCGAATTGCTCCCTGAGCAGGTGGAGGTCTGCGCAGACCTCCACCTGCGGCCCTACTA
CGGTGACGAAGACGACACAGACGGCCTCTATCGCTCCGAGGCCAAGCGTGGAACCACCGCGTTCCACGCCTACGCGACACTCTACGCGCGTGTGAAGAAC
AAACGCTACACGCTGGCGGTGCGCCGTCTCGAAGACGGCGACACCGCCAGCAGCGTCCTCGCGGAGTTCCTCGGACTGCTTGACGGCCTTGACTTTGGTG
TCAAGGCCGTCTACCTCGATCGCGGCTTCTACGACAGCAAGTGTCTCACGCTCCTCCAAGCGCACAACTACGCGTACGTAATCCCGATCATCCGGTGGGG
AAAGACGATTCAGCAGGAACTCTCGAACGGCTGGAGTCGCGTCATTCAACATGACCTGACAGGGACACTCGACGGTCACAGCTGGACCGTCGAGTTTCCC
GTCCACATCGACTGTACGTACCTGAACGGGAAGTATGACGAGAATGGGGTGGCACGTCACGGCTACGCCGCTGACGCGCCGTTTATTGACTCACCACGCG
ACGCTCGATACCACTACAGCAAACGCTTCGGTATCGAGTCGAGCTATCGCTTGTCCGAGCAAGCGATAGCGACAACAACGACGCGAGACGCAACGGTGAG
ACTGCTGTACGTCGTGGTGAGTCTGTTGTTACAGAACGCGTGGCGGTATCTCCACTACGAATACGTGGCGACGCCCCGCCGGGGCGGGCGTCGCCTCTGG
TGGTGGCCGTACAAGGAGTTCATCAATATGATTCGACGTGCTGCGTGGACGGCCCTCGCGGTGCGTCGGGCCGTCCCCGCGAATCGGCCACCTGACGACC
GATTCGATCGCTAACCACCGACCAAGCAAGCCAGCGGAGTGAGTGGCGACGCTGTCGCGTCGGCGGCTGACCGCCGCCGACAGCGACAGCTCTCCATCGA
CCCGTCCGTGATTCTCTCGTCGAGACCGTCAGCACACCCGTCTCGACACAGAACTCAGGCCGCAGAAACAGCTAGCCGAGGATGCTTTGTGAGGTACTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1167 bp388 aa481214+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSRTKQADSEIHEDQLLNFLVNQLDEEVSLGLANNAEIEPEDIYEVLVGACADGTSVSTLCERSEDSPPANTVLYHLRTKFELDWLERVANTLLRRDIVE
LLPEQVEVCADLHLRPYYGDEDDTDGLYRSEAKRGTTAFHAYATLYARVKNKRYTLAVRRLEDGDTASSVLAEFLGLLDGLDFGVKAVYLDRGFYDSKCL
TLLQAHNYAYVIPIIRWGKTIQQELSNGWSRVIQHDLTGTLDGHSWTVEFPVHIDCTYLNGKYDENGVARHGYAADAPFIDSPRDARYHYSKRFGIESSY
RLSEQAIATTTTRDATVRLLYVVVSLLLQNAWRYLHYEYVATPRRGGRRLWWWPYKEFINMIRRAAWTALAVRRAVPANRPPDDRFDR

 

Blast result :
Comments
ISHsal2 is 94% aa similar to ISHla1.
References
1] Pfeiffer F., Marchfelder A., Habermann BH., Dyall-Smith M. (2018) Direct submission.