ISHsal7

  • Family IS66
  • Group ISBst12
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Halobacterium salinarum
Halobacterium salinarum DSM 3754
DNA section
IS Length : 1546 bp

Ends


IR Length : 11

IRL : GTACGTGTTTAGTCCCAGAACGATTTCGGTAGGCTCTCGTAGGAGTCGTC
IRR : GTACGTGTTTACCTCGAGGATGCAACAGCCGGCACAGCCTGAGCTCGTGA

Insertion site


Left flankDirect repeatRight flankDR Length
GCCCAAGAGTATCTCGAAGATACGTACGACGT8

DNA sequence

GTACGTGTTTAGTCCCAGAACGATTTCGGTAGGCTCTCGTAGGAGTCGTCCAACTAGTGCAGTAGATCGATGCAGCAGGCGAACGAGTGCATCTTTGAGG
GATGGTCTCCGGGACGGAGACCATCCCGTCTCGCCTGCTCATGACGTCGTCCGAGCCGTCCAAAGAAGAGCTTCTTACGCAGTTTGCTGAGCTCAAGCAA
GAAGTCGAGGAACTACGGAAGGAGAATCAACGCCTCAGAGCCAAACTCCGGTGGTACGAAGGGCCGCATACACCACCGAGCAAGGACCAATCAGACCAAG
AGGAGTCGTCCTCGTCCGATGGAGACGAGGACGACGAACAGCCACGTACTGACGGTGGCACGCCTGGCCGAAAAGCTGGACACGAGCCAGAATGGCGTGA
TGCCCCTGACCCTAACGAAGAAATCGAGGTCACGTGTGACTGCTGTCCAGAGTGTGGTGAACAATTCGACGAGTCGGCGGGCGTCAGCCCCCGACTCGTC
GAGGAACTCCCAGATCCACAGCCGCCCGAAGTCACACAGTACAATCGTCACCACTACGAGTGCCATTCATGTGGGACCGAGACTGTCGCCTCACACCCCG
ACTGCCCCGATGAGGGGCAGTTCGGGGTGAACGTCATCGCACAGTCAGCGCTTTCGCGGTACGATCACCGGCTTCCCTACCGCAAGATCGGTGACCGCTT
CGAGCAACTACACGGACTGGAGCTGTCCGCCGCATCAACGTGGCACGCGACCGAGCGCGCTGCGCGCGCCGGTCGCTGCGAATACGAGCAGATCCGTCGC
CAGATCCAGCAAGCCGACGTTGTCCACGTTGACGAGACCGGAATCAAACGGGACGGTGAACAGGCGTGGCTGTGGACGTTCACCACGGACGAGCACACGC
TGTACGCGGTCAGAGAGAGTCGTGGGAGTGATGTTCCCGCGGAAGTCCTCGGTGAGGACTTCGCGGGAACGGTCATCTGTGATGGGTGGACGGCGTATCC
AGCCTTCAGCGAGACGCTTCAGCGGTGCTGGGCACACATTCTCCGGGAAGCTGAAGAAGCAGCCGAACAGCAGGATGAAGGGAAACCGATCTACCGTGCT
CTGAGACAGCTGTACGTCGCTCTCCAGGCCCGGCTGGAGAGCGACGTGACAGTGCGCGAGCGGGCAGAACTACAGCGTGTCGCGCGAAGGGAGCTTGAGT
CGCTGATTGAGCGGTCGGTTCCCGACGGACCAGTGGCAACACTGCTCGGGAAAATCGAAGGAGGTCTCGACCACTGGCTCACCTTCGTCGGTGAGCCAGG
GGTCTCCCCGACGAACAACGCTGCGGAAAACGCGCTGCGTGAGCCGGTGGTTCTCCGGAAGCTCATCGGAACGCTCCGTAATGACCGCGGTATGTTCGTT
CACGAGACGCTGCTGTCCCTGCTGGCGACGTGGCGCCAGCAGGGACGCAATCCCTACGAGGAACTCAGGCGAGTCGTCCAGGACAACGAGATGCTTTCAC
GAGCTCAGGCTGTGCCGGCTGTTGCATCCTCGAGGTAAACACGTAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1437 bp478 aa1021538+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVSGTETIPSRLLMTSSEPSKEELLTQFAELKQEVEELRKENQRLRAKLRWYEGPHTPPSKDQSDQEESSSSDGDEDDEQPRTDGGTPGRKAGHEPEWRD
APDPNEEIEVTCDCCPECGEQFDESAGVSPRLVEELPDPQPPEVTQYNRHHYECHSCGTETVASHPDCPDEGQFGVNVIAQSALSRYDHRLPYRKIGDRF
EQLHGLELSAASTWHATERAARAGRCEYEQIRRQIQQADVVHVDETGIKRDGEQAWLWTFTTDEHTLYAVRESRGSDVPAEVLGEDFAGTVICDGWTAYP
AFSETLQRCWAHILREAEEAAEQQDEGKPIYRALRQLYVALQARLESDVTVRERAELQRVARRELESLIERSVPDGPVATLLGKIEGGLDHWLTFVGEPG
VSPTNNAAENALREPVVLRKLIGTLRNDRGMFVHETLLSLLATWRQQGRNPYEELRRVVQDNEMLSRAQAVPAVASSR

 

Blast result :
Comments
ISHsal7 is 95% aa similar to ISNamo3.
References
1] Pfeiffer F., Marchfelder A., Habermann BH., Dyall-Smith M. (2018) Direct submission.