ISKpn50
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| JN420336 | ND | Klebsiella pneumoniae | Klebsiella pneumoniae Klebsiella pneumoniae pNDM-MAR |
DNA section
IS Length : 2455 bp
Ends
IR Length : 14/22
IRL : GTAAGCGTCGTCTCAGCACCGTCTGGCAGATCCTGAAATTCCTGAGAGGA
IRR : GTAAGCGTACAGCGAGAGCCGTATTGACGGGGAGGTGTTATTCAGTTGGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTATGGTGTG | GAGCGGTC | AGTCATTATA | 8 |
DNA sequence
GTAAGCGTCGTCTCAGCACCGTCTGGCAGATCCTGAAATTCCTGAGAGGATAGTGGACACCAAATATGGTGGACACTATCCATGAAATCATTAACCACAG
TGCGTAAAAAAAGCCCTAATTATCCCGTTGAGTTCAAAATCAAAATGGTTGAACTCTCGCATCGACCAGATATCTCCGTAGCGCAACTCGCTCGTGAGCA
TGGGATCAACGATAATCTGCTGTTCAAGTGGCGCCAGTACTGGCGCGAAGGAAAACTACGCCCTCCTTCAACAACAGAAAACAGCGTGCCTGAGCTGCTC
CCCATAACACTTGATGCCGAAGATCTTGTTCCTACAACCTCCTCCCGGTCACAACCTGTAGCTGCTGCGACATCTGAATCACTCAATATCAGCTGTGAAG
TGACGTTCCGGCATGGCTCACTCCGTCTGAATGGTGCCATCAGCGAAAATATCCTGAACCTGCTGATACGGGAGCTCAAACGTTGATCCCATTACCATCA
GGGACAAAGATCTGGCTCGTCGCTGGCATCACCGATATGAGAAACGGCTTCAACGGCCTGGCGGCAAAGGTGCAGACGACGCTGAAAGACGATCCGATGT
CAGGTCACGTTTTTATCTTCCGTGGGCGTAATGGCAGTCAGGTAAAGCTCCTCTGGTCTACCGGCGATGGACTGTGTCTACTGACCAAACGGCTGGAGCG
CGGCCGCTTCGCCTGGCCGTCGGCCCGCGATGGCAAAGTGTTCCTGACCCCGGCACAGCTGGCGATGCTGATGGAAGGTATCGACTGGCGACAACCTAAA
CGGCTGCTGACATCCCTGACCATGCTGTAAATCTCTTTATCCTGGTTGTTGCTGAATAAGCCTGGTAAAATACGGGCTTATGAACGACACCTCTTCTGAC
GAAATCCTTCTGCTGAAACAGCGCCTGGCCGAACAGGAAGCGCTGAACCGTGCCTTGCTGGAGAAACTGGCCGGTCGTGAACGCGAAATAGACCACCTGC
AGGCGCAGCTGGATAAACTCCGCCGGATGAACTTCGGCAGCCGTTCCGAAAAAGTATCCCGTCGTACCGCACAGATGGAAGCTGACCTGAAGGCGCTTCA
GAAAGAAAGCGATACGCTGACCGGTCGGGTGGATGACCCGGCAGTGCAGCGCCCGCTGCGTCAGACCCGCACCCGCAAACCGTTCCCGGAGTCACTTCCT
CGTGACGAAAAGCGACTGCTGCCTGCGGAGTCGTGCTGCCCTGACTGCGGCGGTTCACTGAGCTATCTGGGCGAAAATACCGCCGAACAGCTGGAGCTGG
TGCGCAGCGCCTTCCGGGTTATCCGGACAGTACGTGAAAAGCATGCCTGCACTCAGTGCGATGCCATCGTGCAGGCCCCGGCGCCTTCGCGGCCCATCGA
GCGGGGGATCGCCGGACCGGGGCTGCTGGCCCGCGTGCTGACCTCGAAGTATGCAGAGCACACGCCGCTATATCGGCAGTCAGAGATATACGGCCGGCAG
GGTGTGGAGCTGAGCCGCTCACTGCTGTCGGGCTGGGTGGATGCATGTTGCCGGCTGCTGTCCCCGCTGGAAGAGGCGCTTCAGGGCTATGTCCTGACCG
ACGGCAAACTCCATGCCGATGACACCCCGGTCCAGGTGCTGTTGCCGGGCAATAAGAAGACGAAAACCGGACGGTTGTGGACGTATGTTCGTGACGACCG
CAACGCCGGTTCAGCGCTGGCACCGGCGGTCTGGTTCGCCTACAGCCCGGACAGAAAAGGCATCCATCCGCAGACCCATCTTGCCGGCTTCAGCGGTGTG
CTGCAGGCGGATGCGTACGCCGGGTTCAACGAGCTGTACCGCAATGGCCGGATAACTGAAGCAGCCTGCTGGGCTCATGCCCGCCGAAAGATCCACGATG
TGCACGTCCGCACCCCGTCAGCGCTGACGGAAGACGCCCTGAAGCGTATCGGCGAGCTGTACGCCATAGAGGCGGATATAAGGGGAATGCCGGCGGAGCA
GCGGCTTGCTGAACGTCAGCGAAAAACGAAACCGCTGTTGAAATGCCTGGAAAGCTGGCTGCGTGAAAAGATGAAAACGCTGTCGCGACACTCAGAGCTG
GCAAAAGCGTTCGCGTACGCACTTAACCAGTGGCTGGCACTGACGTACTATGTGGACGATGGCTGGGCAGAAGCAGACAATAATATCGCTGAAAATGCCC
TGCGGACGGTCAGTCTGGGTCGTAAAAACTTCCTGTTCTTCGGCTCTGACCATGGTGGTGAGCGGGGAGCGCTGCTGTACAGCCTGATCGGGACGTGCAA
ACTGAACGGCGTGGATCCAGAAAGCTACCTTCGCCATGTGCTTGACGTCATAGCTGACTGGCCGGTCAACCGGGTCAGTGAACTGCTACCCTGGCGCATC
ACACTGCCAACTGAATAACACCTCCCCGTCAATACGGCTCTCGCTGTACGCTTAC
TGCGTAAAAAAAGCCCTAATTATCCCGTTGAGTTCAAAATCAAAATGGTTGAACTCTCGCATCGACCAGATATCTCCGTAGCGCAACTCGCTCGTGAGCA
TGGGATCAACGATAATCTGCTGTTCAAGTGGCGCCAGTACTGGCGCGAAGGAAAACTACGCCCTCCTTCAACAACAGAAAACAGCGTGCCTGAGCTGCTC
CCCATAACACTTGATGCCGAAGATCTTGTTCCTACAACCTCCTCCCGGTCACAACCTGTAGCTGCTGCGACATCTGAATCACTCAATATCAGCTGTGAAG
TGACGTTCCGGCATGGCTCACTCCGTCTGAATGGTGCCATCAGCGAAAATATCCTGAACCTGCTGATACGGGAGCTCAAACGTTGATCCCATTACCATCA
GGGACAAAGATCTGGCTCGTCGCTGGCATCACCGATATGAGAAACGGCTTCAACGGCCTGGCGGCAAAGGTGCAGACGACGCTGAAAGACGATCCGATGT
CAGGTCACGTTTTTATCTTCCGTGGGCGTAATGGCAGTCAGGTAAAGCTCCTCTGGTCTACCGGCGATGGACTGTGTCTACTGACCAAACGGCTGGAGCG
CGGCCGCTTCGCCTGGCCGTCGGCCCGCGATGGCAAAGTGTTCCTGACCCCGGCACAGCTGGCGATGCTGATGGAAGGTATCGACTGGCGACAACCTAAA
CGGCTGCTGACATCCCTGACCATGCTGTAAATCTCTTTATCCTGGTTGTTGCTGAATAAGCCTGGTAAAATACGGGCTTATGAACGACACCTCTTCTGAC
GAAATCCTTCTGCTGAAACAGCGCCTGGCCGAACAGGAAGCGCTGAACCGTGCCTTGCTGGAGAAACTGGCCGGTCGTGAACGCGAAATAGACCACCTGC
AGGCGCAGCTGGATAAACTCCGCCGGATGAACTTCGGCAGCCGTTCCGAAAAAGTATCCCGTCGTACCGCACAGATGGAAGCTGACCTGAAGGCGCTTCA
GAAAGAAAGCGATACGCTGACCGGTCGGGTGGATGACCCGGCAGTGCAGCGCCCGCTGCGTCAGACCCGCACCCGCAAACCGTTCCCGGAGTCACTTCCT
CGTGACGAAAAGCGACTGCTGCCTGCGGAGTCGTGCTGCCCTGACTGCGGCGGTTCACTGAGCTATCTGGGCGAAAATACCGCCGAACAGCTGGAGCTGG
TGCGCAGCGCCTTCCGGGTTATCCGGACAGTACGTGAAAAGCATGCCTGCACTCAGTGCGATGCCATCGTGCAGGCCCCGGCGCCTTCGCGGCCCATCGA
GCGGGGGATCGCCGGACCGGGGCTGCTGGCCCGCGTGCTGACCTCGAAGTATGCAGAGCACACGCCGCTATATCGGCAGTCAGAGATATACGGCCGGCAG
GGTGTGGAGCTGAGCCGCTCACTGCTGTCGGGCTGGGTGGATGCATGTTGCCGGCTGCTGTCCCCGCTGGAAGAGGCGCTTCAGGGCTATGTCCTGACCG
ACGGCAAACTCCATGCCGATGACACCCCGGTCCAGGTGCTGTTGCCGGGCAATAAGAAGACGAAAACCGGACGGTTGTGGACGTATGTTCGTGACGACCG
CAACGCCGGTTCAGCGCTGGCACCGGCGGTCTGGTTCGCCTACAGCCCGGACAGAAAAGGCATCCATCCGCAGACCCATCTTGCCGGCTTCAGCGGTGTG
CTGCAGGCGGATGCGTACGCCGGGTTCAACGAGCTGTACCGCAATGGCCGGATAACTGAAGCAGCCTGCTGGGCTCATGCCCGCCGAAAGATCCACGATG
TGCACGTCCGCACCCCGTCAGCGCTGACGGAAGACGCCCTGAAGCGTATCGGCGAGCTGTACGCCATAGAGGCGGATATAAGGGGAATGCCGGCGGAGCA
GCGGCTTGCTGAACGTCAGCGAAAAACGAAACCGCTGTTGAAATGCCTGGAAAGCTGGCTGCGTGAAAAGATGAAAACGCTGTCGCGACACTCAGAGCTG
GCAAAAGCGTTCGCGTACGCACTTAACCAGTGGCTGGCACTGACGTACTATGTGGACGATGGCTGGGCAGAAGCAGACAATAATATCGCTGAAAATGCCC
TGCGGACGGTCAGTCTGGGTCGTAAAAACTTCCTGTTCTTCGGCTCTGACCATGGTGGTGAGCGGGGAGCGCTGCTGTACAGCCTGATCGGGACGTGCAA
ACTGAACGGCGTGGATCCAGAAAGCTACCTTCGCCATGTGCTTGACGTCATAGCTGACTGGCCGGTCAACCGGGTCAGTGAACTGCTACCCTGGCGCATC
ACACTGCCAACTGAATAACACCTCCCCGTCAATACGGCTCTCGCTGTACGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 405 bp | 134 aa | 82 | 486 | + | No |
AG : IS66 TnpA
ORF sequence :
MKSLTTVRKKSPNYPVEFKIKMVELSHRPDISVAQLAREHGINDNLLFKWRQYWREGKLRPPSTTENSVPELLPITLDAEDLVPTTSSRSQPVAAATSES
LNISCEVTFRHGSLRLNGAISENILNLLIRELKR
LNISCEVTFRHGSLRLNGAISENILNLLIRELKR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 348 bp | 115 aa | 483 | 830 | + | No |
AG : IS66 TnpB
ORF sequence :
MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVFIFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLMEGI
DWRQPKRLLTSLTML
DWRQPKRLLTSLTML
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1539 bp | 512 aa | 880 | 2418 | + | No |
Chemistry : DDE
ORF sequence :
MNDTSSDEILLLKQRLAEQEALNRALLEKLAGREREIDHLQAQLDKLRRMNFGSRSEKVSRRTAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRK
PFPESLPRDEKRLLPAESCCPDCGGSLSYLGENTAEQLELVRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQ
SEIYGRQGVELSRSLLSGWVDACCRLLSPLEEALQGYVLTDGKLHADDTPVQVLLPGNKKTKTGRLWTYVRDDRNAGSALAPAVWFAYSPDRKGIHPQTH
LAGFSGVLQADAYAGFNELYRNGRITEAACWAHARRKIHDVHVRTPSALTEDALKRIGELYAIEADIRGMPAEQRLAERQRKTKPLLKCLESWLREKMKT
LSRHSELAKAFAYALNQWLALTYYVDDGWAEADNNIAENALRTVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNGVDPESYLRHVLDVIADWPVNRVS
ELLPWRITLPTE
PFPESLPRDEKRLLPAESCCPDCGGSLSYLGENTAEQLELVRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQ
SEIYGRQGVELSRSLLSGWVDACCRLLSPLEEALQGYVLTDGKLHADDTPVQVLLPGNKKTKTGRLWTYVRDDRNAGSALAPAVWFAYSPDRKGIHPQTH
LAGFSGVLQADAYAGFNELYRNGRITEAACWAHARRKIHDVHVRTPSALTEDALKRIGELYAIEADIRGMPAEQRLAERQRKTKPLLKCLESWLREKMKT
LSRHSELAKAFAYALNQWLALTYYVDDGWAEADNNIAENALRTVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNGVDPESYLRHVLDVIADWPVNRVS
ELLPWRITLPTE
Blast result :
Comments
ISKpn50 is 97% (transposase) aa similar to ISCfr14.
References
1] Carattoli,A. (2012) J. Antimicrob. Chemother. 67 (7), 1645-1650.