ISAlw25
- Family IS1
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
CP019143 | ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii ZS207 Acinetobacter lwoffii EK30A plasmid pALWEK1.1 |
DNA section
IS Length : 788 bp
Ends
IR Length : 25/31
IRL : GGTGGTGTATCAAAAAGTATGCTAAAAAAAAGCAGGTTGAATTTTGATAA
IRR : GGTGGTGTTTTAAAAAGTATGCTGACATTAAATGAAGCCATTATTGATGT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
CTCACCAATA AAAATGTC | AAAAACTCTACTATAGCAT | 0 | |
CCCAGAAGTAAAACTTTT | AAAATGTC TAGTTTATGA | 0 |
DNA sequence
GGTGGTGTATCAAAAAGTATGCTAAAAAAAAGCAGGTTGAATTTTGATAAAATAGAGAAATGCAAATAACTCTAGAAATCAAATGTCCAACCTGCCTCAG
TGACAGTATAAAGAAAAATGGCATCAAAGTAGATGGGAAACAAAACTACCAATGCAAAGACTGCAAACGTCAGTTTATTGGTGACCATGCTCTGAGCTAT
CTAGGATGTAATTCTGGCATTACTCGTAAAATATTACAGTTAATGGTCAGAGGCAGCGGTATACGAGATATCGCTGAAGTTGAGCGCATTAGTATCGGTA
AAGTCTTACGGACTTTAACTGAATCGGCCTATCAAATTCAGCCTAAACAAAGTCATTATGAATCTCTCGAAGTAGATGAATTCTGGACTTTTGTTGGAAA
TAAAAATAATAAACAATGGCTTATTTACGCCTACCATCGAGAAACAGGTGAGATTGTTGCTTATGTTTGGGGTAAGAGAGATTTAGCTACAGTCCAAAGG
TTGAAGACAAAGCTTAAACAATTAGGTATTCACTACACCCGAATTGCAAGTGATCATTGGGATAGTTTCATCACTGCTTTTAAAAACTGCAAGCAAAGTA
TTGGTAAGTTTTTTACTGTAGGAATTGAAGGTAATAATTGCAAAATAAGGCATCGAATAAGGCGTGGTTTTAGAAGAAGTTGTAATTTCTCCAAAAAGCT
TGAAAACCATTTTAAAGCCTTCGACTTAACCTTCTTTTACATCAATAATGGCTTCATTTAATGTCAGCATACTTTTTAAAACACCACC
TGACAGTATAAAGAAAAATGGCATCAAAGTAGATGGGAAACAAAACTACCAATGCAAAGACTGCAAACGTCAGTTTATTGGTGACCATGCTCTGAGCTAT
CTAGGATGTAATTCTGGCATTACTCGTAAAATATTACAGTTAATGGTCAGAGGCAGCGGTATACGAGATATCGCTGAAGTTGAGCGCATTAGTATCGGTA
AAGTCTTACGGACTTTAACTGAATCGGCCTATCAAATTCAGCCTAAACAAAGTCATTATGAATCTCTCGAAGTAGATGAATTCTGGACTTTTGTTGGAAA
TAAAAATAATAAACAATGGCTTATTTACGCCTACCATCGAGAAACAGGTGAGATTGTTGCTTATGTTTGGGGTAAGAGAGATTTAGCTACAGTCCAAAGG
TTGAAGACAAAGCTTAAACAATTAGGTATTCACTACACCCGAATTGCAAGTGATCATTGGGATAGTTTCATCACTGCTTTTAAAAACTGCAAGCAAAGTA
TTGGTAAGTTTTTTACTGTAGGAATTGAAGGTAATAATTGCAAAATAAGGCATCGAATAAGGCGTGGTTTTAGAAGAAGTTGTAATTTCTCCAAAAAGCT
TGAAAACCATTTTAAAGCCTTCGACTTAACCTTCTTTTACATCAATAATGGCTTCATTTAATGTCAGCATACTTTTTAAAACACCACC
Protein section
ORF number : 1
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
702 bp | 233 aa | 60 | 761 | + | No |
Chemistry : DDE
ORF sequence :
MQITLEIKCPTCLSDSIKKNGIKVDGKQNYQCKDCKRQFIGDHALSYLGCNSGITRKILQLMVRGSGIRDIAEVERISIGKVLRTLTESAYQIQPKQSHY
ESLEVDEFWTFVGNKNNKQWLIYAYHRETGEIVAYVWGKRDLATVQRLKTKLKQLGIHYTRIASDHWDSFITAFKNCKQSIGKFFTVGIEGNNCKIRHRI
RRGFRRSCNFSKKLENHFKAFDLTFFYINNGFI
ESLEVDEFWTFVGNKNNKQWLIYAYHRETGEIVAYVWGKRDLATVQRLKTKLKQLGIHYTRIASDHWDSFITAFKNCKQSIGKFFTVGIEGNNCKIRHRI
RRGFRRSCNFSKKLENHFKAFDLTFFYINNGFI
Blast result :
Comments
ISAlw25 is 94% similar to ISPa14. Probable transposon composite with DR at each end of the transposon.
References
1] Janos Kiss (2019) Direct submission.
2] Alexandra Veress, Tibor Nagy, Tímea Wilk, János Kömüves, Ferenc Olasz & János Kiss. (2020) Sci Rep 10, 2969.
2] Alexandra Veress, Tibor Nagy, Tímea Wilk, János Kömüves, Ferenc Olasz & János Kiss. (2020) Sci Rep 10, 2969.