ISAlw9

  • Family IS4
  • Group IS4
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter lwoffii
Acinetobacter lwoffii
Acinetobacter lwoffii M2a
DNA section
IS Length : 1392 bp

Ends


IR Length : 18

IRL : TAATGCCAGTCAGTTAAGCAGACATTAGAAAAATGTAGTAAAAATGAGTG
IRR : TAATGCCAGTCAGTTAAGAAATTGACTGGCATTTTCTTGGGTATTTCTGT

Insertion site


Left flankDirect repeatRight flankDR Length
gcggataaaggtaaaaaacccctcaatcgaggggctttaaattaaatagctt12
attgaattgacataaaagctctcattaatgagggcttttttattaagtgaaa12

DNA sequence

TAATGCCAGTCAGTTAAGCAGACATTAGAAAAATGTAGTAAAAATGAGTGTATGCCAATACGCTCATTTTTTTTATGACTTTATCTGAAAATTTAGATTG
CACCCTTCAACATTCTTTGCCTTCACTCAGCCATTTCAGTGAACTCATTGATTTAAACTGGATTAAAGAAAGCCTACATCAAACAGGTAAGGCTTCTATC
AGGAGAAGAAAATTACCTGCCGAACATGTGGTATGGCTGGTAATTGGGCTTGCCCTATTTCGAGATCAACCGATCGGGTATGTCGTAGAGCAACTAAAAC
TTGTGTTTGGTACAACAGAATATTGTGTCCCCAGTGCAGCAGTGCAAGCACGACAACGTTTAGGACGAGAGCCCTTGAATACCTTGTTTTCTCTACTCAG
CCAAGCCTGGTTTGAAGAATCCCAACAGCAATACTCAAACTTTCATGGCCTTAGTGTATGTGCTGTTGACGGGGTGGTTTGGTCTATGCCTTATACAGAC
GAGAATTTTGAACACTTTGGCTCATCTAAGGGTAAAACCGCTGCAGCCCCTTATCCACAAGTGAGAGCAACCTGCCTGGTAAATACCAGCACCCATGAAA
TCATTGATGCCCAAATAGGTAGCATGGATCAGGGTGAACTTACACTGGCAAATCGATTATGTCCTCCATCGCATAGTATTACCTTGTTTGATCGAGCCTA
TTTCTCTGCTGATTTCTTGATTGACTGGCAAACACGAGTAGAAGCCAGTCATTGGTTAATGAGAGCAAAAGATAATTTACGCTATGAGATCATTAAACGT
AATTCCCCGCATGACTTTCAAATCAAAATGCCTCTTTCAGCTAGAGCCAGGAAGCTAAATCCATCATTAGGAGAATATTGGGAAGCGCGTTTGATTGAAG
TTGAGCAGGCAGGGAAAATTAGACGTTATATCACTTCATTAATGGATTCAAAGGCATATCCACTGATAGGCTTGGCGAAGCTTTATGCCCAGCGCTGGGA
AATAGAAATGTGTTACCGAGAAATCAAAAGTGATTTACAGGAAGGCAAGCATTTGAGGAGCAAGCAACCTGATTTAATTTATCAAGAATTATGGGGGGTC
TTCATTGCCTATAATATTCTAAGAAGACAAATGAAACATATGGCTCAACGAGCAAAAGTCAGTCCTTTGAGAATCAGCTTTCATATTGCATCTATCGGTA
TCCTGAATATATTAAGATTTGACTCTTTAGACTCCGCAGGGAATTTACCCAAACATCTAGAAAGTTTACTGGAGAAATCTAAGAGGTATGTGTTGCCTGA
GAGGAGGGTTAGAAGTTGCCCACGAGTTGTGAAAGGAAAACCACAGAAATACCCAAGAAAATGCCAGTCAATTTCTTAACTGACTGGCATTA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1305 bp434 aa751379+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTLSENLDCTLQHSLPSLSHFSELIDLNWIKESLHQTGKASIRRRKLPAEHVVWLVIGLALFRDQPIGYVVEQLKLVFGTTEYCVPSAAVQARQRLGREP
LNTLFSLLSQAWFEESQQQYSNFHGLSVCAVDGVVWSMPYTDENFEHFGSSKGKTAAAPYPQVRATCLVNTSTHEIIDAQIGSMDQGELTLANRLCPPSH
SITLFDRAYFSADFLIDWQTRVEASHWLMRAKDNLRYEIIKRNSPHDFQIKMPLSARARKLNPSLGEYWEARLIEVEQAGKIRRYITSLMDSKAYPLIGL
AKLYAQRWEIEMCYREIKSDLQEGKHLRSKQPDLIYQELWGVFIAYNILRRQMKHMAQRAKVSPLRISFHIASIGILNILRFDSLDSAGNLPKHLESLLE
KSKRYVLPERRVRSCPRVVKGKPQKYPRKCQSIS

 

Blast result :
Comments
ISAlw9 is 58% aa similar to ISEch9.
References
1] Janos Kiss (2019) Direct submission.
2] Alexandra Veress, Tibor Nagy, Tímea Wilk, János Kömüves, Ferenc Olasz & János Kiss. (2020) Sci Rep 10, 2969.