ISAlw10
- Family IS4
- Group IS4
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
CP019143.1 | ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii M2a Acinetobacter lwoffii ZS207 Acinetobacter lwoffii NCTC5866 |
DNA section
IS Length : 1389 bp
Ends
IR Length : 18
IRL : TAATGCCAGTCAGTTAAGCAAAAAAAGTTAAAATGCTGTAAAAAGAGCGT
IRR : TAATGCCAGTCAGTTAAGAAATTGACTGGCATTTTCTTGGATATTTTTGT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
gacccagcaataaaaaagcc | tctagagataga | ggcttttttatatttaaaac | 12 |
tgctctgctattaaaaagcc | ctcaattgcatg | gggcttttttatgtttgagt | 12 |
CTGAAGATATAAAAAAGC | CCTCAGTTGAG | GGCTTTTTTATATGCATT | 11 |
ATAGACATAAAAAAAGCC | TCTACGACTAGG | GGCTTTTTTTATTTATAA | 12 |
TAATAAATATCAAAAAAG | CTCAATTGAGGG | CTTTTTTTAGGCTGGTAG | 12 |
TTCTCAAAACAAAAAGCC | CGCTTTACGCA | GGCTTTTTATCCGAAAAA | 11 |
TCTAGAATGAAAAAAGCC | CTCTAATGAG | GGCTTTTTCTTTTAAAAA | 10 |
DNA sequence
TAATGCCAGTCAGTTAAGCAAAAAAAGTTAAAATGCTGTAAAAAGAGCGTATGTAAATACGCTCTTTTTTTATGAATTTATCTCAGAATCTAGATTTAAC
ATTAAAACAAACCCTACCTTCACTTTCACAATTCAGTGAATTGATTGATTTAAACTGGATTGAAGATTGCTTAGACCAAACAGGTAAAGCATCAATTCGA
AAAAGAAAACTGCCTGCTGAACATGTTGTTTGGCTGGTAATCGGACTTGCTCTATTTCGAAATCAACCGATTTGGTATGTGGTTCAACAATTGCAACTTG
TTTTCGGTACGGCAGAATACTGTGTACCGAGTGCATCCGTACAAGCAAGACAACGCTTAGGCTTAGAACCCATGAGTGCTTTATTTTCGACATTAAGTCA
GGCATGGTTTAAAGACTCACAACAACAATATAGCAACTTTCACGGTCTATGCGTTTGTGCTGTAGATGGTGTGGTTTGGTCTATGCCTCATACAGAAGAA
AACTTTAAGCACTTTGGTTCATCCAAAGGCAAAACAGCAGCTGCACCTTACCCACAAGTTAGAGCGACTTGCCTGGTGAATACCAATACCCATGAAATGA
TTGATGCCCAAATGGGCAGTATGGATCAAGGTGAATTAACCTTAGCCAGTCAATTAAAAGCACCAGTTCGCAGTATTACCCTATTTGATCGTGCTTACTT
CTCTGCTGATTTTTTAGTGAGCTGGCAATCTCAGGCAGAAGAGAGTCATTGGTTGATGCGAGCAAAGGACAACCTGCGTTATGAAGTGATTCATCATAAT
GCGGCCCATGACTTTCAGATCAAAATGCCTGTTTCAGCAAGAGCAAAAAAGATAAATCCGTCATTGGGTGACTATTGGGAAGCACGTTTAATTGAAGTTG
AATATGCAGGAAAAATAAGACGTTACATTACATCATTAACAGATTCTAAAGTTTATCCATTCAAAGACCTTGCAATGCTTTATATCCAGCGTTGGGAAAT
AGAAATGTGTTATCGGGAAATTAAAAGTGATTTACAGGATGCAAGGATTTTAAGAAGCAAACAACCTGATTTGGTCTATCAAGAATTGTGGGGGGTATTT
ATTGCATATAATATCTTAAGAAGACAGATGAGGTTTATCGCTGAACATGCAAAGGTGAGTCCTTTAAGGATCAGTTTCCATATTGCATCTATGAGTATTA
TCAATATCTTAAGGCACACACCTTTAGAATCAGCAGGAAACCTACCCAAACATTTAGCGCAATTATTTGAACAATCTAAAATATTTGTATTACCTGAAAA
AAGGCAAAGGCAATGTCCGCGAGTAGTGAAAATTAAAGCACAAAAATATCCAAGAAAATGCCAGTCAATTTCTTAACTGACTGGCATTA
ATTAAAACAAACCCTACCTTCACTTTCACAATTCAGTGAATTGATTGATTTAAACTGGATTGAAGATTGCTTAGACCAAACAGGTAAAGCATCAATTCGA
AAAAGAAAACTGCCTGCTGAACATGTTGTTTGGCTGGTAATCGGACTTGCTCTATTTCGAAATCAACCGATTTGGTATGTGGTTCAACAATTGCAACTTG
TTTTCGGTACGGCAGAATACTGTGTACCGAGTGCATCCGTACAAGCAAGACAACGCTTAGGCTTAGAACCCATGAGTGCTTTATTTTCGACATTAAGTCA
GGCATGGTTTAAAGACTCACAACAACAATATAGCAACTTTCACGGTCTATGCGTTTGTGCTGTAGATGGTGTGGTTTGGTCTATGCCTCATACAGAAGAA
AACTTTAAGCACTTTGGTTCATCCAAAGGCAAAACAGCAGCTGCACCTTACCCACAAGTTAGAGCGACTTGCCTGGTGAATACCAATACCCATGAAATGA
TTGATGCCCAAATGGGCAGTATGGATCAAGGTGAATTAACCTTAGCCAGTCAATTAAAAGCACCAGTTCGCAGTATTACCCTATTTGATCGTGCTTACTT
CTCTGCTGATTTTTTAGTGAGCTGGCAATCTCAGGCAGAAGAGAGTCATTGGTTGATGCGAGCAAAGGACAACCTGCGTTATGAAGTGATTCATCATAAT
GCGGCCCATGACTTTCAGATCAAAATGCCTGTTTCAGCAAGAGCAAAAAAGATAAATCCGTCATTGGGTGACTATTGGGAAGCACGTTTAATTGAAGTTG
AATATGCAGGAAAAATAAGACGTTACATTACATCATTAACAGATTCTAAAGTTTATCCATTCAAAGACCTTGCAATGCTTTATATCCAGCGTTGGGAAAT
AGAAATGTGTTATCGGGAAATTAAAAGTGATTTACAGGATGCAAGGATTTTAAGAAGCAAACAACCTGATTTGGTCTATCAAGAATTGTGGGGGGTATTT
ATTGCATATAATATCTTAAGAAGACAGATGAGGTTTATCGCTGAACATGCAAAGGTGAGTCCTTTAAGGATCAGTTTCCATATTGCATCTATGAGTATTA
TCAATATCTTAAGGCACACACCTTTAGAATCAGCAGGAAACCTACCCAAACATTTAGCGCAATTATTTGAACAATCTAAAATATTTGTATTACCTGAAAA
AAGGCAAAGGCAATGTCCGCGAGTAGTGAAAATTAAAGCACAAAAATATCCAAGAAAATGCCAGTCAATTTCTTAACTGACTGGCATTA
Protein section
ORF number : 1
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1305 bp | 434 aa | 72 | 1376 | + | No |
Chemistry : DDE
ORF sequence :
MNLSQNLDLTLKQTLPSLSQFSELIDLNWIEDCLDQTGKASIRKRKLPAEHVVWLVIGLALFRNQPIWYVVQQLQLVFGTAEYCVPSASVQARQRLGLEP
MSALFSTLSQAWFKDSQQQYSNFHGLCVCAVDGVVWSMPHTEENFKHFGSSKGKTAAAPYPQVRATCLVNTNTHEMIDAQMGSMDQGELTLASQLKAPVR
SITLFDRAYFSADFLVSWQSQAEESHWLMRAKDNLRYEVIHHNAAHDFQIKMPVSARAKKINPSLGDYWEARLIEVEYAGKIRRYITSLTDSKVYPFKDL
AMLYIQRWEIEMCYREIKSDLQDARILRSKQPDLVYQELWGVFIAYNILRRQMRFIAEHAKVSPLRISFHIASMSIINILRHTPLESAGNLPKHLAQLFE
QSKIFVLPEKRQRQCPRVVKIKAQKYPRKCQSIS
MSALFSTLSQAWFKDSQQQYSNFHGLCVCAVDGVVWSMPHTEENFKHFGSSKGKTAAAPYPQVRATCLVNTNTHEMIDAQMGSMDQGELTLASQLKAPVR
SITLFDRAYFSADFLVSWQSQAEESHWLMRAKDNLRYEVIHHNAAHDFQIKMPVSARAKKINPSLGDYWEARLIEVEYAGKIRRYITSLTDSKVYPFKDL
AMLYIQRWEIEMCYREIKSDLQDARILRSKQPDLVYQELWGVFIAYNILRRQMRFIAEHAKVSPLRISFHIASMSIINILRHTPLESAGNLPKHLAQLFE
QSKIFVLPEKRQRQCPRVVKIKAQKYPRKCQSIS
Blast result :
Comments
ISAlw10 77% similar to ISAlw9.
A. lwoffii NCTC5866 (LR215977.1), A. johnsonii M19 (CP037424.1) and Acinetobacter sp. NCu2D-2 (CP015594.1) carry 7, 4 and 14 copies of ISAlw10 (99% similar to the prototype).
A. lwoffii NCTC5866 (LR215977.1), A. johnsonii M19 (CP037424.1) and Acinetobacter sp. NCu2D-2 (CP015594.1) carry 7, 4 and 14 copies of ISAlw10 (99% similar to the prototype).
References
1] Janos Kiss (2019) Direct submission.
2] Alexandra Veress, Tibor Nagy, Tímea Wilk, János Kömüves, Ferenc Olasz & János Kiss. (2020) Sci Rep 10, 2969.
2] Alexandra Veress, Tibor Nagy, Tímea Wilk, János Kömüves, Ferenc Olasz & János Kiss. (2020) Sci Rep 10, 2969.