ISAlw13

  • Family IS5
  • Group IS427
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter lwoffii
Acinetobacter lwoffii
Acinetobacter lwoffii M2a
DNA section
IS Length : 811 bp

Ends


IR Length : 17

IRL : GGGCGTGTCATCAATTACCTTGAGGAAAACCACCTTGTCCCCATTATACT
IRR : GGGCGTGTCATCAATTAATGGAGATGATTGTTGATGCTAAGTAGATCGCT

Insertion site


Left flankDirect repeatRight flankDR Length
ctcgaaatatggatcgaatctacataagatctataaaaagct2
tgaaataaatcatggggcccTAgatagcacagcatcttattt2

DNA sequence

GGGCGTGTCATCAATTACCTTGAGGAAAACCACCTTGTCCCCATTATACTTTTATGACTAAACGCTATGCCTTAAGAGATGATCAATGGGAACAGATTAA
AGATCTGTTACCTGGACGAATGGGTACTGTTGGAGTAACGGCCAAAGACAACCGACTATTTGTTGAAGCAGTATTGTATCGGTATCGTTCAGGTATCCCG
TGGCGAGACTTACCAGAACGCTTTGGTGATTTTAGAGTCGTGCATACTCGTTTTAGCCGTTGGGCTAAAACAGGTGTGTGGCAGAGGGTCTTTGAAGTGC
TGTCACATGACAGTGATAATGAATACGCCATGTTAGACTCCACCATTGTACGTGCTCATCAACACAGCGCTGGGAAAAAAAAATCAGGCGATTGGACGCA
GTAAAGGTGGCTTAAGCACCAAAATTCATGCCCGTACTGATGCTTTAGGCAACCCAACAGGATTTTATCTCACTGATGGGCAGGCCCATGACTTAAACGG
TTCAGATGTGTTGCTGGACTTATCGTTGAGCCAGACCTGGCTTATGGATAAAGCCTACAACTCCAGGGATCGGGTCATTGCTCCAATTTTAGCGGCGAAG
GGTCAAATTGTGATGCCAGCGAAAAGTAATTCTATTGATCAGCGTGATTATGACCGACATCTCTACAAGGCAAGACATCTGATCGAGAACTTTTTTGCCA
AGCTCAAGCAGTATCGTGCTATTGCCACACGCTATGATAAATTGGCTCAAAACTTCTTATCAGCGATCTACTTAGCATCAACAATCATCTCCATTAATTG
ATGACACGCCC
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
351 bp116 aa54404+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTKRYALRDDQWEQIKDLLPGRMGTVGVTAKDNRLFVEAVLYRYRSGIPWRDLPERFGDFRVVHTRFSRWAKTGVWQRVFEVLSHDSDNEYAMLDSTIVR
AHQHSAGKKKSGDWTQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
465 bp168 aa337801+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TPPLYVLINTALGKKNQAIGRSKGGLSTKIHARTDALGNPTGFYLTDGQAHDLNGSDVLLDLSLSQTWLMDKAYNSRDRVIAPILAAKGQIVMPAKSNSI
DQRDYDRHLYKARHLIENFFAKLKQYRAIATRYDKLAQNFLSAIYLASTIISIN

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
748 bp263 aa54801+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTKRYALRDDQWEQIKDLLPGRMGTVGVTAKDNRLFVEAVLYRYRSGIPWRDLPERFGDFRVVHTRFSRWAKTGVWQRVFEVLSHDSDNEYAMLDSTIVR
AHQHSAGKKTPPLYVLINTALGKKNQAIGRSKGGLSTKIHARTDALGNPTGFYLTDGQAHDLNGSDVLLDLSLSQTWLMDKAYNSRDRVIAPILAAKGQI
VMPAKSNSIDQRDYDRHLYKARHLIENFFAKLKQYRAIATRYDKLAQNFLSAIYLASTIISIN

 

Blast result :Comments : The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
Comments
ISAlw13 is 83% similar to ISPssp5.
References
1] Janos Kiss (2019) Direct submission.