ISAlw31

  • Family IS5
  • Group IS427
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP019144 ND Acinetobacter lwoffii
Acinetobacter lwoffii
Acinetobacter lwoffii ZS207 plasmid pmZS
DNA section
IS Length : 811 bp

Ends


IR Length : 17

IRL : GAGCCTGTCATCAATTAGCTTGAGAAAATCTAACCTGTCCTCATTATTTT
IRR : GGGCGTGTCATCAATTAAGCCATATCATGATTGAAGCAAGGTAGATTGCT

Insertion site


Left flankDirect repeatRight flankDR Length
TATTTAATAATAGCAAACTAAGCATAATAGGCACAAA2

DNA sequence

GAGCCTGTCATCAATTAGCTTGAGAAAATCTAACCTGTCCTCATTATTTTTTCATGACTAAACGCTATGCATTAAGAGATGATCAGTGGGAGCAGATTAA
GGATCTGTTACCTGGACGAGCGAGTACTGTTGGAGTAACTGCCAAAGATAACAGGCTATTTGTTGAAGCAGTATTGTATCGGTATCGTTCAGGTATTCCA
TGGCGAGACTTACCCGAATGCTTTGGGGATTTTAGAGTTGTGCACACTCGCTTTAGCCGTTGGGTTAAAGCAGGCGTATGGCAGAAAGTTTTTGAAGTGC
TATCACATGAAAGTGATAATGAATATGCCATGCTAGACTCAACCATTGTGCGTGCTCATCAACACAGCGCCGGAAAAAAAAGAACAGGCAATCGGACGCA
GTAAAGGTGGTTTAAGTACCAAAATTCATGCCCGTACTGATGCTTTAGGCAACCCAACAGGATTTTATCTCACTGCCGGGCAGACTCATGACTTAAATGG
TGCTGATGTGCTGCTGGACTTATCGTTAAGTCAGACTTGGCTCATGGATAAAGCCTATAATTCTAAGGATCGGGTGATTGATCCAATCCATAAGATCAAG
GGTCAGATCGTGATGTCATCCAAAAGTAACGCGATTGATCAGCGTGATTATGACCAACATCTTTATAAAGCAAGACATCTGATCGAGAACTTCTTCGCCA
AGCTCAAGCAGTATCGAGGTATTGCTACGCGCTATGATAAATTGGCTCAAAACTTCCTGTCAGCAATCTACCTTGCTTCAATCATGATATGGCTTAATTG
ATGACACGCCC
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
351 bp116 aa54404+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTKRYALRDDQWEQIKDLLPGRASTVGVTAKDNRLFVEAVLYRYRSGIPWRDLPECFGDFRVVHTRFSRWVKAGVWQKVFEVLSHESDNEYAMLDSTIVR
AHQHSAGKKRTGNRTQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
465 bp154 aa337801+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

TQPLCVLINTAPEKKEQAIGRSKGGLSTKIHARTDALGNPTGFYLTAGQTHDLNGADVLLDLSLSQTWLMDKAYNSKDRVIDPIHKIKGQIVMSSKSNAI
DQRDYDQHLYKARHLIENFFAKLKQYRGIATRYDKLAQNFLSAIYLASIMIWLN

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
748 bp248 aa54801+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTKRYALRDDQWEQIKDLLPGRASTVGVTAKDNRLFVEAVLYRYRSGIPWRDLPECFGDFRVVHTRFSRWVKAGVWQKVFEVLSHESDNEYAMLDSTIVR
AHQHSAGKKEQAIGRSKGGLSTKIHARTDALGNPTGFYLTAGQTHDLNGADVLLDLSLSQTWLMDKAYNSKDRVIDPIHKIKGQIVMSSKSNAIDQRDYD
QHLYKARHLIENFFAKLKQYRGIATRYDKLAQNFLSAIYLASIMIWLN

 

Blast result :
Comments
ISAlw31 is 86% aa similar to ISPssp5.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift. ISAlw31 is 87% similar to ISAlw13. Its closest relative (96% similar) occurs in Acinetobacter sp. ACNIH1 (CP026420.1).
References
1] Alexandra Veress, Tibor Nagy, Tímea Wilk, János Kömüves, Ferenc Olasz & János Kiss. (2020) Sci Rep 10, 2969.