ISAlw34
- Family IS66
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
CP019143.1 | ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii ZS207 plasmid pmZS |
DNA section
IS Length : 2511 bp
Ends
IR Length : 16
IRL : GTAAGCGTCCGCTGAACACACCTTATGAATTCATCCTATAAGCCTTAATT
IRR : GTAAGCGTCCGCTGAATCCCATACCAATTTTTAATTCGATTTAGGTTTCC
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
gagctttgatgcggacattg | aattactc | gcattgcatagagatagata | 8 |
DNA sequence
GTAAGCGTCCGCTGAACACACCTTATGAATTCATCCTATAAGCCTTAATTTAGTCCCCACTTGAAAACAAGTGGGGACTAAAATTTATGACTACAAATCA
CCAGACATCCATCGCATCTCTTGCGAAAAAACGAAGAACATACAGTGCTGAATTTAAACAGCAGATCGTTCAGGCTTGTAAAGCACCAGACGTTTCAATT
GCTTCGGTCGCTTTGCAACATGGATTGAATACAAATCTTGTATCCAAATGGATTCGCTTAATTGATGGTAAGCCAGGGAATGATCGCTCACCACTACCGA
ATAAACCTGCATTTATTGCCTTATCTTGCTCTGCACCATTAGATCCTACTCCTACTGACATGTTAACGGTTCAAATTACTTTACCCCACTCAAAAGCAGA
AATTGGCTTGAAATGGCAAGTATCAGAAATATCTGCTTTAGCAGAATTACTCAAGGCACTTGCAACATGATCCGCATTGATGAAATCTGGCTTTCTACCC
AACCTCTGGATATGCGAGCAGGGATGGATACTATCATGGCTCAGGTGGTGAGAGCCTTTGGCTACATTAAACCGCATTGTGCTTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTGCTGGTACATGATGGACTGGGCATCTGGCTGTGTACCCGGCGGCTGGAACAGGGAAAATTCCACTGGGCTAAAGTTCAC
CAGGGTGAAAACGTAGCTCTCAGTCCAGAACAGTTACAGGCACTAATTCAGGGTTTGCCCTGGCAGCGCATTGGACGACAGCAAGTGGTAACAATGCTCT
AAACTAGGCTGCTACATTCTGCTATTCTCCAAACGTTCTATTTCCTCTGCGTCATGACCTCAGGCATACTGCGGTCATGAAAATGCTGCCTGATTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTTACCCGGCAGTTGGCGATGCAGCATCAGTCTCTGGCACAATCAAATCAAGAATTAGAAAAATCAAACCAGC
AATTGGATGCCAAAGTTCAGCATCTTTCTATTCTAAATCAAAAATACGAGCATGAACTGGCGCTGTTTAAAAATCATAAATTCGGCAGTAAAAACGAACA
TCTTACCGCAAAACAAATCCATCTATGGGACGAAGCGGTCGAAGAAGATATCGCAGCCGTTGATCTAGAATTAGAACGATTAAATGCAGATAAAACCGAT
GCAGTGACACAGAATGCCACAGTCAACAAACCTAAACGTCGACTGCTGCCAGATCATCTACACACCATCCGTATTGAGCATGAACCTGCATCAACCCAAT
GCAGTTGTGGCTGCCAGTTACGTCGTATCGGCGAAGATATCAGTGAAAAACTGCATTTCAGACCGGCACAGTTCTACAAGGAACAGCATGTCCGTGGCAA
ATGGGTCTGTGATCAGTGCGACACTCTGACTCAGCAAGCGATGCCTGCCTATGTGATTGATAAAGGCATTGCTTCACCTGAATTGCTCAGCCATGTGCTG
GTATCGAAGTATGCCGATCATTTGCCGCTGTACCGTCAGCGCCTGATCTATCAGCGGGCGGGCATTGATCTGTCCAGATCTACTTTATCTGACTGGATTG
GTCGCTGCGGTGTGGAACTGGAACCTCTGGCCAATGCCTTAAAACAGGTGGTGCTGCAACAGCGGGTGATCCATGCCGATGAAACGCCGGTCACCATCAT
GCGGATGGGTGATGATGAGAAAAAACCGAAGAAAGGTTATGTCTGGGCCTATGCCACGACACAGTACAATCCAGTTCAGGCAGTGATCTATGACTTTCAG
GATAGTCGTTCAGGTCAGCATGCTGAAGCGTTCCTGAATGGCTGGCAGGGCTATCTAGTCTGTGATGATTACAGTGGTTATAAAGCACGCTTTAAATCAG
GCCAGGTCATTGAGGTGGGCTGCATGGCCCATGCACGTCGTAAATTCCATGAACTGCATGTGACCGGGAAAAGTCAGGTCGCTGAACAGGCATTGGTGCT
GATTCAGAAACTGTATGCGATAGAAGCAGAACTCAGGAAAAAGACCGATGGTACAGCAGAAGACCGCCGCGAATACCGACAACAGCATAGTCAACCAGTG
ATGCAACAACTATATGAATGGCTCAACCAACATCATCTGACAGTGCCATCGAGTTCTCCCACCGCCAAGGCGATCAATTACACTCTGAAGCGTTGGTCAG
CTTTAAGCCGCTATCTGGATGATGGCAATCTACCGATTTGCAATAATTGGGTCGAGAATCAGATGCGTCCCTGGGCGTTGGGGCGCAAGAACTGGCTGTT
TGCAGGTTCGCTGCGCAGTGGTCAGCGAGCGGCAAACATCATGACGTTAATCCAGTCAGCAAAGCTGAATGGGCTGGATCCGTATGCTTATTTAAGTGAT
GTGCTGAAAAGGCTGCCGACACATAAAGTGACCCAGATTGAAGAGTTACTGCCACACTGCTGGAAACCTAAATCGAATTAAAAATTGGTATGGGATTCAG
CGGACGCTTAC
CCAGACATCCATCGCATCTCTTGCGAAAAAACGAAGAACATACAGTGCTGAATTTAAACAGCAGATCGTTCAGGCTTGTAAAGCACCAGACGTTTCAATT
GCTTCGGTCGCTTTGCAACATGGATTGAATACAAATCTTGTATCCAAATGGATTCGCTTAATTGATGGTAAGCCAGGGAATGATCGCTCACCACTACCGA
ATAAACCTGCATTTATTGCCTTATCTTGCTCTGCACCATTAGATCCTACTCCTACTGACATGTTAACGGTTCAAATTACTTTACCCCACTCAAAAGCAGA
AATTGGCTTGAAATGGCAAGTATCAGAAATATCTGCTTTAGCAGAATTACTCAAGGCACTTGCAACATGATCCGCATTGATGAAATCTGGCTTTCTACCC
AACCTCTGGATATGCGAGCAGGGATGGATACTATCATGGCTCAGGTGGTGAGAGCCTTTGGCTACATTAAACCGCATTGTGCTTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTGCTGGTACATGATGGACTGGGCATCTGGCTGTGTACCCGGCGGCTGGAACAGGGAAAATTCCACTGGGCTAAAGTTCAC
CAGGGTGAAAACGTAGCTCTCAGTCCAGAACAGTTACAGGCACTAATTCAGGGTTTGCCCTGGCAGCGCATTGGACGACAGCAAGTGGTAACAATGCTCT
AAACTAGGCTGCTACATTCTGCTATTCTCCAAACGTTCTATTTCCTCTGCGTCATGACCTCAGGCATACTGCGGTCATGAAAATGCTGCCTGATTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTTACCCGGCAGTTGGCGATGCAGCATCAGTCTCTGGCACAATCAAATCAAGAATTAGAAAAATCAAACCAGC
AATTGGATGCCAAAGTTCAGCATCTTTCTATTCTAAATCAAAAATACGAGCATGAACTGGCGCTGTTTAAAAATCATAAATTCGGCAGTAAAAACGAACA
TCTTACCGCAAAACAAATCCATCTATGGGACGAAGCGGTCGAAGAAGATATCGCAGCCGTTGATCTAGAATTAGAACGATTAAATGCAGATAAAACCGAT
GCAGTGACACAGAATGCCACAGTCAACAAACCTAAACGTCGACTGCTGCCAGATCATCTACACACCATCCGTATTGAGCATGAACCTGCATCAACCCAAT
GCAGTTGTGGCTGCCAGTTACGTCGTATCGGCGAAGATATCAGTGAAAAACTGCATTTCAGACCGGCACAGTTCTACAAGGAACAGCATGTCCGTGGCAA
ATGGGTCTGTGATCAGTGCGACACTCTGACTCAGCAAGCGATGCCTGCCTATGTGATTGATAAAGGCATTGCTTCACCTGAATTGCTCAGCCATGTGCTG
GTATCGAAGTATGCCGATCATTTGCCGCTGTACCGTCAGCGCCTGATCTATCAGCGGGCGGGCATTGATCTGTCCAGATCTACTTTATCTGACTGGATTG
GTCGCTGCGGTGTGGAACTGGAACCTCTGGCCAATGCCTTAAAACAGGTGGTGCTGCAACAGCGGGTGATCCATGCCGATGAAACGCCGGTCACCATCAT
GCGGATGGGTGATGATGAGAAAAAACCGAAGAAAGGTTATGTCTGGGCCTATGCCACGACACAGTACAATCCAGTTCAGGCAGTGATCTATGACTTTCAG
GATAGTCGTTCAGGTCAGCATGCTGAAGCGTTCCTGAATGGCTGGCAGGGCTATCTAGTCTGTGATGATTACAGTGGTTATAAAGCACGCTTTAAATCAG
GCCAGGTCATTGAGGTGGGCTGCATGGCCCATGCACGTCGTAAATTCCATGAACTGCATGTGACCGGGAAAAGTCAGGTCGCTGAACAGGCATTGGTGCT
GATTCAGAAACTGTATGCGATAGAAGCAGAACTCAGGAAAAAGACCGATGGTACAGCAGAAGACCGCCGCGAATACCGACAACAGCATAGTCAACCAGTG
ATGCAACAACTATATGAATGGCTCAACCAACATCATCTGACAGTGCCATCGAGTTCTCCCACCGCCAAGGCGATCAATTACACTCTGAAGCGTTGGTCAG
CTTTAAGCCGCTATCTGGATGATGGCAATCTACCGATTTGCAATAATTGGGTCGAGAATCAGATGCGTCCCTGGGCGTTGGGGCGCAAGAACTGGCTGTT
TGCAGGTTCGCTGCGCAGTGGTCAGCGAGCGGCAAACATCATGACGTTAATCCAGTCAGCAAAGCTGAATGGGCTGGATCCGTATGCTTATTTAAGTGAT
GTGCTGAAAAGGCTGCCGACACATAAAGTGACCCAGATTGAAGAGTTACTGCCACACTGCTGGAAACCTAAATCGAATTAAAAATTGGTATGGGATTCAG
CGGACGCTTAC
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
384 bp | 127 aa | 87 | 470 | + | No |
AG : IS66 TnpA
ORF sequence :
MTTNHQTSIASLAKKRRTYSAEFKQQIVQACKAPDVSIASVALQHGLNTNLVSKWIRLIDGKPGNDRSPLPNKPAFIALSCSAPLDPTPTDMLTVQITLP
HSKAEIGLKWQVSEISALAELLKALAT
HSKAEIGLKWQVSEISALAELLKALAT
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
390 bp | 129 aa | 413 | 802 | + | No |
AG : IS66 TnpB
ORF sequence :
MASIRNICFSRITQGTCNMIRIDEIWLSTQPLDMRAGMDTIMAQVVRAFGYIKPHCAYLFCNKRGHRMKVLVHDGLGIWLCTRRLEQGKFHWAKVHQGEN
VALSPEQLQALIQGLPWQRIGRQQVVTML
VALSPEQLQALIQGLPWQRIGRQQVVTML
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1605 bp | 534 aa | 877 | 2481 | + | No |
Chemistry : DDE
ORF sequence :
MKMLPDLSQLTHEQLLEFTRQLAMQHQSLAQSNQELEKSNQQLDAKVQHLSILNQKYEHELALFKNHKFGSKNEHLTAKQIHLWDEAVEEDIAAVDLELE
RLNADKTDAVTQNATVNKPKRRLLPDHLHTIRIEHEPASTQCSCGCQLRRIGEDISEKLHFRPAQFYKEQHVRGKWVCDQCDTLTQQAMPAYVIDKGIAS
PELLSHVLVSKYADHLPLYRQRLIYQRAGIDLSRSTLSDWIGRCGVELEPLANALKQVVLQQRVIHADETPVTIMRMGDDEKKPKKGYVWAYATTQYNPV
QAVIYDFQDSRSGQHAEAFLNGWQGYLVCDDYSGYKARFKSGQVIEVGCMAHARRKFHELHVTGKSQVAEQALVLIQKLYAIEAELRKKTDGTAEDRREY
RQQHSQPVMQQLYEWLNQHHLTVPSSSPTAKAINYTLKRWSALSRYLDDGNLPICNNWVENQMRPWALGRKNWLFAGSLRSGQRAANIMTLIQSAKLNGL
DPYAYLSDVLKRLPTHKVTQIEELLPHCWKPKSN
RLNADKTDAVTQNATVNKPKRRLLPDHLHTIRIEHEPASTQCSCGCQLRRIGEDISEKLHFRPAQFYKEQHVRGKWVCDQCDTLTQQAMPAYVIDKGIAS
PELLSHVLVSKYADHLPLYRQRLIYQRAGIDLSRSTLSDWIGRCGVELEPLANALKQVVLQQRVIHADETPVTIMRMGDDEKKPKKGYVWAYATTQYNPV
QAVIYDFQDSRSGQHAEAFLNGWQGYLVCDDYSGYKARFKSGQVIEVGCMAHARRKFHELHVTGKSQVAEQALVLIQKLYAIEAELRKKTDGTAEDRREY
RQQHSQPVMQQLYEWLNQHHLTVPSSSPTAKAINYTLKRWSALSRYLDDGNLPICNNWVENQMRPWALGRKNWLFAGSLRSGQRAANIMTLIQSAKLNGL
DPYAYLSDVLKRLPTHKVTQIEELLPHCWKPKSN
Blast result :
Comments
ISAlw34 is 95% aa (transposase) similar to ISAlw16 and ISAba46.
References
1] Janos Kiss (2019) Direct submission.
2] Alexandra Veress, Tibor Nagy, Tímea Wilk, János Kömüves, Ferenc Olasz & János Kiss. (2020) Sci Rep 10, 2969.
2] Alexandra Veress, Tibor Nagy, Tímea Wilk, János Kömüves, Ferenc Olasz & János Kiss. (2020) Sci Rep 10, 2969.