ISAlw17

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter lwoffii
Acinetobacter lwoffii
Acinetobacter lwoffii M2a
DNA section
IS Length : 877 bp

Ends


IR Length : 15

IRL : TATAGCCAAATACTTAAAAATATAGAACAATAGAAACATGGGATATTCAA
IRR : TATAGCTAAATACTAAATAAATCATTACAGTAATCTTTAAACAATTTATC

Insertion site


Left flankDirect repeatRight flankDR Length
attcacgaatttctgtgtcaTAgctacagaattgtgctgtat0
gctataccaatatcacaaacTAgttcactattcttctgagaa0

DNA sequence

TATAGCCAAATACTTAAAAATATAGAACAATAGAAACATGGGATATTCAAAAGATTTTAAAGACAAAGTCATAGAAATTATGACTAGAGATAAAATGTCT
GTGCGAAAAGCAGCACAGCATTTTAACGTCTGTATACAGACGATTCAGAATTGGAAAAAGTCCACTGTAACTAAACCTATTCCTGGTCGCCCAGCTAAAA
TTAGCAAAGAGCAAATATTGAATGATGTTGAACAATATCCCGATGATTACATCAGTGAACGGGCAGAACGTTTTGGTGTCAGTACACATGCAGTATTTAA
TGCGCTACATGCTGCAGGAATATCGCGTAAAAAAAGACGTTAAAGCACCCTAAAGCAGATCAAAATCAACGTCGGCAATTTCAGAACTTGATTGAATGTT
TAGAACAACAATTACCACTGGTTTATATCGATGAAAGTGGTTTTCAAAGCGAAAGTACGAGATCGCATAGTTATAGCCCTAAAGGAGCGCGAGCTGAAGC
TGAATTTAACTGGCAGTTAAAAAATTGTAGCAATGCGATTGGCGCATTAATCGAAAATAGATTGCTTACAGTTGGAGTATTTGATTGTTCAGTGAATACC
GAGGTATTTGAATGCTGGATTGAACAGGATTTATTGCCAAAGCTGAAAAAAACCAGTGTATTGATTATGGATAATGCCACCTTTCACAAAGGAACAAGGC
TTCAGGAGTTGATACGTTGTGCAGGCCATTACATTGTGTGGCTTCCTAAATATAGCCCGGACTTAAATCCTATAGAAAAAATGTGGTCACGAGTAAAAAT
GATTCGCAATAAATTTAGAGTTAAAGATATTGATAAATTGTTTAAAGATTACTGTAATGATTTATTTAGTATTTAGCTATA
Recoding section
  • Recoding by frameshift
  • Frame +1
  • Type transcriptional
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
406 bp101 aa36441+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MGYSKDFKDKVIEIMTRDKMSVRKAAQHFNVCIQTIQNWKKSTVTKPIPGRPAKISKEQILNDVEQYPDDYISERAERFGVSTHAVFNALHAAGISRKKR
R

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
546 bp181 aa329874+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KKTLKHPKADQNQRRQFQNLIECLEQQLPLVYIDESGFQSESTRSHSYSPKGARAEAEFNWQLKNCSNAIGALIENRLLTVGVFDCSVNTEVFECWIEQD
LLPKLKKTSVLIMDNATFHKGTRLQELIRCAGHYIVWLPKYSPDLNPIEKMWSRVKMIRNKFRVKDIDKLFKDYCNDLFSI

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
839 bp278 aa36874+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGYSKDFKDKVIEIMTRDKMSVRKAAQHFNVCIQTIQNWKKSTVTKPIPGRPAKISKEQILNDVEQYPDDYISERAERFGVSTHAVFNALHAAGISRKKT
LKHPKADQNQRRQFQNLIECLEQQLPLVYIDESGFQSESTRSHSYSPKGARAEAEFNWQLKNCSNAIGALIENRLLTVGVFDCSVNTEVFECWIEQDLLP
KLKKTSVLIMDNATFHKGTRLQELIRCAGHYIVWLPKYSPDLNPIEKMWSRVKMIRNKFRVKDIDKLFKDYCNDLFSI

 

Blast result :
Comments
ISAlw17 is 63% aa (transposase) similar to ISAba44.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible +1 frameshift.
References
1] Alexandra Veress, Tibor Nagy, Tímea Wilk, János Kömüves, Ferenc Olasz & János Kiss. (2020) Sci Rep 10, 2969.