ISAlw18

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter lwoffii
Acinetobacter lwoffii
Acinetobacter lwoffii M2a
DNA section
IS Length : 1078 bp

Ends


IR Length : 24

IRL : agttgacaaaaataactaatgatgcgaaaaggtttttttagttaacagat
IRR : agtatccattaaaaagtaaacatgtattcttttccgaattggtttgatac

Insertion site


Left flankDirect repeatRight flankDR Length
gaacttctatttaataacgaTAaatgtcacactttgtttaaa2
aaactaaaataatcttcacaTAgcactataactaaaatatct2
atagataaagcgaaagataaTAttgctatggtatctgttgaa2
agtcttcaggagacactgcaTAagagtttaaagaaataaata2

DNA sequence

AGTTGACAAAAATAACTAATGATGCGAAAAGGTTTTTTTAGTTAACAGATGTTCACACATGCCTAGAATTGTTTCTGTACCACTTAGTTTAGAACAGCGA
GAACGACTCATTTTTCTGGTCAAGCATGCAAAACATTGGCGTGAAAGGCAACGTGCCCAAACTATTCTCTGGCTTTCCGAAGGTAAAAGTGTTGCGGAAG
TTGCCACTCTACAAGAACGTATCCCAGAAACCATAAGGCTACAACGCCGACGTTGGGAATTGTATGAGCTTGAATCGATTAAAGAAGGTCATCGCTCAGG
TCGACCCAATACATTGATCTCAGACTATCAGGCGAAAATCCTAGATTGGGTCAATACAAGTCCACTCAATGCAGAACAAATCCGAGTCAAACTACATGAA
GAATACGAAGTGTCCGTGTCTGTTGAAACCATTCGTAAGTTTTTACGTGATTCAGGTATGGTCTTCAAGCGTACCCGTCATAGCTTGAAAAAAAAGAGAT
CCGATTGCATTTGAACAAGCAACACAGCAGATTGAAGAATTACGAGAGCAAGCGGCACGTGGTGAAATTATATTAGGTTATGTTGATGAAACAGGATTTT
CATCTACACCTGATAACCGCTATGCGTGGACAAAGATAGGTGATGTTCATGCGGTTGATGCAATAAGATTAAAACGAGTTAATGTCATGGGGTGTTTGCT
TTCAACGGGGAAACTGGTGACTAGTTGTTTACAAGAGTCAGTGACAAGCACTTGGTTTTATGCCTATTTAACTGGAATAGCTCAACAAGTAAAACAGACA
TATAACCTTCCATTGGTTCTTATTGTTGATAATGCCTCGATTCATAGAAGTAAAAAGATGGCTGATTACAGAGAGCTACTTAAAACTAACTTTTCGACGA
ATCTGTATTTTATTCCAGCTTATAGCCCTGAGTTAAATCGAATCGAGATGGTATGGAAGCAGATGAAATATTATTGGCGAGATTTTCAAGTCATGACAGC
TGACAAAATAGAGCAATGGGTGGAGAAAGTATCAAACCAATTCGGAAAAGAATACATGTTTACTTTTTAATGGATACT
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
456 bp151 aa59514+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPRIVSVPLSLEQRERLIFLVKHAKHWRERQRAQTILWLSEGKSVAEVATLQERIPETIRLQRRRWELYELESIKEGHRSGRPNTLISDYQAKILDWVNT
SPLNAEQIRVKLHEEYEVSVSVETIRKFLRDSGMVFKRTRHSLKKKRSDCI

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
582 bp193 aa4891070+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KKRDPIAFEQATQQIEELREQAARGEIILGYVDETGFSSTPDNRYAWTKIGDVHAVDAIRLKRVNVMGCLLSTGKLVTSCLQESVTSTWFYAYLTGIAQQ
VKQTYNLPLVLIVDNASIHRSKKMADYRELLKTNFSTNLYFIPAYSPELNRIEMVWKQMKYYWRDFQVMTADKIEQWVEKVSNQFGKEYMFTF

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1012 bp336 aa591070+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPRIVSVPLSLEQRERLIFLVKHAKHWRERQRAQTILWLSEGKSVAEVATLQERIPETIRLQRRRWELYELESIKEGHRSGRPNTLISDYQAKILDWVNT
SPLNAEQIRVKLHEEYEVSVSVETIRKFLRDSGMVFKRTRHSLKKRDPIAFEQATQQIEELREQAARGEIILGYVDETGFSSTPDNRYAWTKIGDVHAVD
AIRLKRVNVMGCLLSTGKLVTSCLQESVTSTWFYAYLTGIAQQVKQTYNLPLVLIVDNASIHRSKKMADYRELLKTNFSTNLYFIPAYSPELNRIEMVWK
QMKYYWRDFQVMTADKIEQWVEKVSNQFGKEYMFTF

 

Blast result :
Comments
ISAlw18 is 50% aa (transposase) similar to ISAcma15.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
One of the 5 ISAlw18 copies in strain M2a has a divergent IRR (agttgtcattaaaaagtaaacatg). Acinetobacter wuhouensis strain WCHAW010062 chromosome (CP033133.1) contains 27 intact and 1 interrupted copies of this variant.
References
1] Janos Kiss (2019) Direct submission.