ISAlw4

  • Family IS3
  • Group IS51
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
KX426227 ND Acinetobacter lwoffii
Acinetobacter lwoffii
Acinetobacter lwoffii M2a
Acinetobacter lwoffii ED23-35 pALWED1.1
DNA section
IS Length : 1309 bp

Ends


IR Length : 25

IRL : TGAACCGTACCGGGTTTGTCGGAGAGTCAATATTCTGAGAGACTATCCCG
IRR : TGAACCGTACCGGGTTTGTCGGAGACTTTTTTATTTAAGTTAGGCCACCT

Insertion site


Left flankDirect repeatRight flankDR Length
ACCGTTCCATAGAAAAATAGGTACGGTGCTAAAACAC3

DNA sequence

TGAACCGTACCGGGTTTGTCGGAGAGTCAATATTCTGAGAGACTATCCCGATGACAAAATTAAAATATACCCCTGAAATCAGAGAAAGAGCGGTTCAATT
ATTGATTGAATCTGAAAAAGATTACCCTTCTACTTGGGCTGCAATCACAGCAATTGCGCCTAAGATTGGTTGTACTCCTGAAACACTTCGTGCCTGGCAT
CAAAAGCATTTAGATAAACAAAATCCAGTTAAAGTACAGCAGCTTTCAGACCAAGAACGTATCAAACAACTGGAACGCGAAAATAAAGAACTGCAACGCG
CCAATGAAATTCTACGTAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTCATCCATAATAATAAAGAGCTG
TACGGAGTCGAGGCGATTTGTAGGATTTTACCGATCGCAGCTTCAACCTATTACCGGACTCTAGATCTCTGCGAAAATCCAGAACATCGAGCAAAGCGAG
ATTTACATGACTTGCATCATGCTGAGGAGATTAAACGAATTTGGAAGGAAAGTTCAGGTCGGTATGGTGTACGTAAAGTCTGGCAACAATTGAAACGTGA
AGGCTATATTATTGCACGCTGTACTGTTGCTCGATTAATGAAAAAGCTAGGTATACAAGGTGTTTGGCGAGGTAAGAATAAACAAACCACCCGTAGCCGA
GATGATCAAAAAAGAGCAGATGATTTAGTGAAACGTAATTTTAATGCTGATCATCCTGACCAACTGTGGGTGAGTGACTTTACGTATATTCAAACTCATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTCTCACGAGCAATTGTTGGATGGAAAGTATCTACACGGATGAATACAGATATGGTACTCGATGC
ATTGGAGCAAGCATTGCACGATCGAGGCATGCCAAAGAATGTGATTCATCATTCCGATAGAGGTGTTCAATATCTTTCTATTCGCTATACCAATCGTTTA
GAAGCTGCAAATTTACGAGCATCAGTCGGTACAACGGGTGATTCATACGATAATGCTCTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCAGATTGGCAAGGTTTAGCAGATGTACAACTTGCGACACTAAACTGGGTAGATTGGTTCAACAAAAAGCGTGTACACAGTGCACTGGG
TTATGTATCGCCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCCTAACTTAAATAAAAAAGTCTCCGACAAACCCGG
TACGGTTCA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
319 bp105 aa50368+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTKLKYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRAWHQKHLDKQNPVKVQQLSDQERIKQLERENKELQRANEILRKAAAFFAQAEL
DRPHK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
952 bp316 aa3191270+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SSRFFRPGGARPPTQIMVDFIHNNKELYGVEAICRILPIAASTYYRTLDLCENPEHRAKRDLHDLHHAEEIKRIWKESSGRYGVRKVWQQLKREGYIIAR
CTVARLMKKLGIQGVWRGKNKQTTRSRDDQKRADDLVKRNFNADHPDQLWVSDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALH
DRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFE
FEAMYYDKINPLGQVA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1221 bp406 aa501270+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTKLKYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRAWHQKHLDKQNPVKVQQLSDQERIKQLERENKELQRANEILRKAAAFFRPGGA
RPPTQIMVDFIHNNKELYGVEAICRILPIAASTYYRTLDLCENPEHRAKRDLHDLHHAEEIKRIWKESSGRYGVRKVWQQLKREGYIIARCTVARLMKKL
GIQGVWRGKNKQTTRSRDDQKRADDLVKRNFNADHPDQLWVSDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFEFEAMYYDKIN
PLGQVA

 

Blast result :
Comments
ISAlw4 is 98% aa (transposase) similar to ISAba34.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
References
1] Janos Kiss (2019) Direct submission.