ISAlw5

  • Family IS3
  • Group IS51
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter lwoffii
Acinetobacter lwoffii
Acinetobacter lwoffii M2a
DNA section
IS Length : 1309 bp

Ends


IR Length : 25

IRL : TGAACCGTACCGGGTTTGTCGGAGACTCAATATTCTGAGAGACTATTCCG
IRR : TGAACCGTACCGGGTTTGTCGGAGACTTTTTTATTTAAGTTAAGCCACCT

Insertion site


Left flankDirect repeatRight flankDR Length
gaaatcaggtcgtcctcatactttagatatagaggatcagttg3

DNA sequence

TGAACCGTACCGGGTTTGTCGGAGACTCAATATTCTGAGAGACTATTCCGATGAAAAAACCAACCTATACCCCCGAAATTAGAGAAAGAGCGGTTCAATT
ACTAATTGAATCTGAAAAAGATTATCCTTCTACTTGGGCTGCAATCACAGCAATTGCGCCTAAGATTGGTTGTACTCCTGAAACACTTCGTGCCTGGCAT
CAAAAGCATTTAGATCAGCAAAATCCTATTAAAGTACAACAGATATCTGATCAAGAAAAAATGAAGCAAATGGAACGTGAAATTAAAGAATTAAAGCGTG
CCAATGAAATTCTACGTAAAGCAGCCGCTTTTTTCGCCTAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTCATCCATAACAATAAAGACTTA
TATGGTGTTGATGCGATTTGTAGGATTTTACCGATCGCAGCTTCAACCTATTACCGAACTTTAGATCTCGCTGACAATCCAGAACATCGAGCGAAACGAG
ATCTACATGATGAGCATCATGCTGAACAAATTAAACGAATTTGGAAGGAAAGTTTAGGTCGATATGGTGTACGTAAAGTTTGGCAAAAATTGAAACGTGA
AGGCTATGTTATTGGACGTTGTACAGTTGCTCGATTGATGCAGAAGCTAGATATACAAGGTGTTTGGCGTGGTAAGAACAAACAAACTACCCGCAACCGA
GATGATCAAAAACGAGCAGATGATTTAGTGAAACGTAATTTTAGTGCTGATCGACCTGACCAATTATGGGTCAGTGACTTTACGTATATTCAAACTCATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTCTCACGAGCAATTGTTGGATGGAAAGTATCTACACGGATGAATACAGATATGGTGCTCGATGC
ACTTGAGCAAGCATTGCACGATCGAGGCATGCCAAAGAATGTGATTCATCATTCCGACAGGGGTGTGCAATATCTTTCGATTCGCTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACAACTGGTGATTCATACGATAATGCTTTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCAGATTGGCAAGGTTTAGCAGATGTACAACTTGCGACACTAAACTGGGTAGATTGGTTCAATAAAAAGCGTGTACACAGTGCACTGGG
TTATGTGTCGCCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCTTAACTTAAATAAAAAAGTCTCCGACAAACCCGG
TACGGTTCA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
318 bp96 aa51368+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKKPTYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRAWHQKHLDQQNPIKVQQISDQEKMKQMEREIKELKRANEILRKAAAFFA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
951 bp316 aa3201270+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SSRFFRLGGARPPTQIMVDFIHNNKDLYGVDAICRILPIAASTYYRTLDLADNPEHRAKRDLHDEHHAEQIKRIWKESLGRYGVRKVWQKLKREGYVIGR
CTVARLMQKLDIQGVWRGKNKQTTRNRDDQKRADDLVKRNFSADRPDQLWVSDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALH
DRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFE
FEAMYYDKINPLGQVA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1220 bp406 aa511270-No

ORF sequence :

MKKPTYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRAWHQKHLDQQNPIKVQQISDQEKMKQMEREIKELKRANEILRKAAAFFRLGGA
RPPTQIMVDFIHNNKDLYGVDAICRILPIAASTYYRTLDLADNPEHRAKRDLHDEHHAEQIKRIWKESLGRYGVRKVWQKLKREGYVIGRCTVARLMQKL
DIQGVWRGKNKQTTRNRDDQKRADDLVKRNFSADRPDQLWVSDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFEFEAMYYDKIN
PLGQVA

 

Blast result :
Comments
ISAlw5 is 96% aa (transposase) similar to ISAba19.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
References
1] Janos Kiss (2019) Direct submission.