ISAlw6
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii M2a |
DNA section
IS Length : 1200 bp
Ends
IR Length : 33/46
IRL : GGTAATGCTCCCTAAAAATAATTGAACTTAAAAGTAGAAAATCGAATAGC
IRR : GGTAATACCCCCATTTTTAATTGAGTTAATGAGTAGAAAAACCGAAGCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| tataaaaaagatgatgagtc | ataaggatctggttttagat | 0 |
DNA sequence
GGTAATGCTCCCTAAAAATAATTGAACTTAAAAGTAGAAAATCGAATAGCCTTGATCTAAGGAGATTTTCTATGAAAACATCTAAATATTCGGACAGCTT
AATCATGAGTATTTTGAAGCAAGCTGAAGCAGGTACTCCAGTGCCTAATCTATGCCGAGAACATGGCATGAGTTCTGCCACTTTTTACAAATGGAAGTCC
AAATATGGCGGCATGGATCTTTCCATGATGACTCGACTTAAAGAGCTAGAGACAGAAAATGCCCGCCTAAAGCGCATGTATGCGGATGAACGTCTTAAAG
CAGACATTCTTCAGGATGCACTGTCAAAAAAGTTATAAGGCCAACGCAACGCAAGTCACTTGCTCAACAAGCCGTTGAGCAATATAAGATATCAGTGAAT
AAGGCCTGTCTCATATTCCAAGTCAGTCAAACCTGTTATCGCTATATAGCTCAATTGAAGGAGCAGAATATGGTTATTGCAGATTGGCTGATTAAGCTCA
CCAATGAGAATAAGACTTGGGGATTTAAGCTTTGCTTTTTGTACCTGCGTAATGTCCAAGGCTACCCTTGGAATCACAAGCGTATTTATCGCATTTATAA
GGAGCTTGAGCTTAACCTTCGGATTAAACCCAACAAACGCATTAAACGGATAAAGCCTGAAACTCTTGCAGTACCAGAAGCGCAGAATGAATCATGGTCA
ATGGACTTTATGCACGATCAACTCAGTGATGGCCGCAGTTACCGTGTGCACAACGTCATTGATGACTATAACCGTGAGTCATTAGATATCTTGATTGACT
TCTCCTTACCCGCCGAACGAGTACTTCGAGGGTTGGATCAACTCATCGAATGGCGAGGTAAACCAAAACGAATCCGAAGTGACAATGGACCAGAGTACAT
CAGTGATTTAATGGAGGCATGGTGTAAGCAGCATGAAATTGAGCATGTATTTACCCAGCCTGGCAATCCACAACAAAATGCGTATGTAGAACGCTTCAAT
CGGACAGTACGTTATGAATGTTTAAATCAATATTTATTTCGAGAACTTGAAGATGTTCAAGACTATACAACAGCATGGCAACACTTTTATAATCATCAAC
GACCTCATATGTCAGTTGATGGTAAACCACCTAAATTTAAACAATGATAAAGGCTTCGGTTTTTCTACTCATTAACTCAATTAAAAATGGGGGTATTACC
AATCATGAGTATTTTGAAGCAAGCTGAAGCAGGTACTCCAGTGCCTAATCTATGCCGAGAACATGGCATGAGTTCTGCCACTTTTTACAAATGGAAGTCC
AAATATGGCGGCATGGATCTTTCCATGATGACTCGACTTAAAGAGCTAGAGACAGAAAATGCCCGCCTAAAGCGCATGTATGCGGATGAACGTCTTAAAG
CAGACATTCTTCAGGATGCACTGTCAAAAAAGTTATAAGGCCAACGCAACGCAAGTCACTTGCTCAACAAGCCGTTGAGCAATATAAGATATCAGTGAAT
AAGGCCTGTCTCATATTCCAAGTCAGTCAAACCTGTTATCGCTATATAGCTCAATTGAAGGAGCAGAATATGGTTATTGCAGATTGGCTGATTAAGCTCA
CCAATGAGAATAAGACTTGGGGATTTAAGCTTTGCTTTTTGTACCTGCGTAATGTCCAAGGCTACCCTTGGAATCACAAGCGTATTTATCGCATTTATAA
GGAGCTTGAGCTTAACCTTCGGATTAAACCCAACAAACGCATTAAACGGATAAAGCCTGAAACTCTTGCAGTACCAGAAGCGCAGAATGAATCATGGTCA
ATGGACTTTATGCACGATCAACTCAGTGATGGCCGCAGTTACCGTGTGCACAACGTCATTGATGACTATAACCGTGAGTCATTAGATATCTTGATTGACT
TCTCCTTACCCGCCGAACGAGTACTTCGAGGGTTGGATCAACTCATCGAATGGCGAGGTAAACCAAAACGAATCCGAAGTGACAATGGACCAGAGTACAT
CAGTGATTTAATGGAGGCATGGTGTAAGCAGCATGAAATTGAGCATGTATTTACCCAGCCTGGCAATCCACAACAAAATGCGTATGTAGAACGCTTCAAT
CGGACAGTACGTTATGAATGTTTAAATCAATATTTATTTCGAGAACTTGAAGATGTTCAAGACTATACAACAGCATGGCAACACTTTTATAATCATCAAC
GACCTCATATGTCAGTTGATGGTAAACCACCTAAATTTAAACAATGATAAAGGCTTCGGTTTTTCTACTCATTAACTCAATTAAAAATGGGGGTATTACC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 72 | 338 | + | No |
Description : First part of the transposase
ORF sequence :
MKTSKYSDSLIMSILKQAEAGTPVPNLCREHGMSSATFYKWKSKYGGMDLSMMTRLKELETENARLKRMYADERLKADILQDALSKKL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 852 bp | 282 aa | 299 | 1150 | + | No |
Description : Second part of the transposase
ORF sequence :
SRHSSGCTVKKVIRPTQRKSLAQQAVEQYKISVNKACLIFQVSQTCYRYIAQLKEQNMVIADWLIKLTNENKTWGFKLCFLYLRNVQGYPWNHKRIYRIY
KELELNLRIKPNKRIKRIKPETLAVPEAQNESWSMDFMHDQLSDGRSYRVHNVIDDYNRESLDILIDFSLPAERVLRGLDQLIEWRGKPKRIRSDNGPEY
ISDLMEAWCKQHEIEHVFTQPGNPQQNAYVERFNRTVRYECLNQYLFRELEDVQDYTTAWQHFYNHQRPHMSVDGKPPKFKQ
KELELNLRIKPNKRIKRIKPETLAVPEAQNESWSMDFMHDQLSDGRSYRVHNVIDDYNRESLDILIDFSLPAERVLRGLDQLIEWRGKPKRIRSDNGPEY
ISDLMEAWCKQHEIEHVFTQPGNPQQNAYVERFNRTVRYECLNQYLFRELEDVQDYTTAWQHFYNHQRPHMSVDGKPPKFKQ
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1079 bp | 358 aa | 72 | 1150 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTSKYSDSLIMSILKQAEAGTPVPNLCREHGMSSATFYKWKSKYGGMDLSMMTRLKELETENARLKRMYADERLKADILQDALSKKVIRPTQRKSLAQQ
AVEQYKISVNKACLIFQVSQTCYRYIAQLKEQNMVIADWLIKLTNENKTWGFKLCFLYLRNVQGYPWNHKRIYRIYKELELNLRIKPNKRIKRIKPETLA
VPEAQNESWSMDFMHDQLSDGRSYRVHNVIDDYNRESLDILIDFSLPAERVLRGLDQLIEWRGKPKRIRSDNGPEYISDLMEAWCKQHEIEHVFTQPGNP
QQNAYVERFNRTVRYECLNQYLFRELEDVQDYTTAWQHFYNHQRPHMSVDGKPPKFKQ
AVEQYKISVNKACLIFQVSQTCYRYIAQLKEQNMVIADWLIKLTNENKTWGFKLCFLYLRNVQGYPWNHKRIYRIYKELELNLRIKPNKRIKRIKPETLA
VPEAQNESWSMDFMHDQLSDGRSYRVHNVIDDYNRESLDILIDFSLPAERVLRGLDQLIEWRGKPKRIRSDNGPEYISDLMEAWCKQHEIEHVFTQPGNP
QQNAYVERFNRTVRYECLNQYLFRELEDVQDYTTAWQHFYNHQRPHMSVDGKPPKFKQ
Blast result :
Comments
ISAlw6 is 96% aa (transpôsase) similar to ISAbe6.
The third ORF is the putative ORFAB transposase reconstructed by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed by possible -1 frameshift.
References
1] Janos Kiss (2019) Direct submission.
2] Alexandra Veress, Tibor Nagy, Tímea Wilk, János Kömüves, Ferenc Olasz & János Kiss. (2020) Sci Rep 10, 2969.
2] Alexandra Veress, Tibor Nagy, Tímea Wilk, János Kömüves, Ferenc Olasz & János Kiss. (2020) Sci Rep 10, 2969.