ISKpn52
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Klebsiella pneumoniae | Klebsiella pneumoniae Klebsiella pneumoniae KP1276 plasmid pIA/C-KLUC |
DNA section
IS Length : 1226 bp
Ends
IR Length : 28/40
IRL : TGTTGTGCTTTAATAAATGTGGACACTTTGATAAAGGAATCTATTCACCT
IRR : TGTTCTGCACAACAAAATTAGGACACTTTTCTGAAGGAGTTTTCGTACGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TACACAA | CCT | TTTACTG | 3 |
| GGTTTCGCATCAGTAAAA | GG | TTGTGTATTCAGAACAAA | 2 |
DNA sequence
TGTTGTGCTTTAATAAATGTGGACACTTTGATAAAGGAATCTATTCACCTTTAAAATAAGATGTCGATATGAAACGAAAATCACCTAAGCAATATACTGA
AAGTTTTAAAAAAGAAGCGATTAATTTAATACTCGAACAAGGCTATACCGTTCAACAAGCGGCTAATGCACTCGGAATTACCACTAAATTGCTCTATGCT
TGGCGAAAGCGCCATGAAGCAGAAAATAAGCCTAACGCATTAACCCCTTTTGAGCGACAAGAGCTACTCGCTCTGCGCAAAGAAGTTAAGCAATTGAAAA
TGGAGAAAGAAATCCTAAAAAAGGCGAGAGTGCCTTCTTTGCGAAAGAGTTGCTATAAGGTGTTGGTTTGTTCAAGAGCATAAAACACAATATCCCATTA
AGCATTTATGTCGAGTGATGAAGCTCAGCCGTTCAACGTTTTATACTTGGCAAAAGCGCCCGGTTAAGCCACTTGATGATGACACGCTTGAATTGCATCA
AAAAGCTTGCTCCCTCTTTAGGGAAAGCCGGCAAAGCTTAGGTTATCGCATGTTAGCGGCTCAACTACGCAAAGAAGGTTATGCTATCAGTGATTATCGA
GCTCGAAAATTAATGAAGCAGTTAGGGTTAGTGGTTAAGCAAAGAAAACAATATCGAGTCACCTCGAAAAATAAAGCGACTGCGGATAATTTATTAAATC
AAAATTTTAATCCGGTAGCAGCGAATGAAGTGTGGGCTGGGGATATCACTTATTTAAAGACACCGGAAGGTTGGCTCTATCTGGCTATCGTGATGGATTT
ATATTCTCGGCGTATTGTGGGTTGGCACATGTCATCCAAAATAGATACTGCCTTGATAAGCAAAGCGTTAATGATGGCCTATAACTTACGTTCGCCCTCA
AAGGGTTGTGTGTTTCACAGTGACCGAGGAAGCCAATACACAAGTGGGCAATATCAAGCGTTACTGAAAAGTTATGATATGCGCTCAAGTCAAGGTGATA
TAGGGGCTTGCTGGGATAATGCGGTAGTTGAACGATTTTTTGGAAGTTTAAAACATGATTGGTTATTTAAAAGACCCCATGCAAATCGGTCAGAAATGAA
ACAAGACGTGCTGGATTATCTGCGTTATTACAATTTAACGCGATTACACACGGCAAATAATTATTTATCGCCTGTGGCGTACGAAAACTCCTTCAGAAAA
GTGTCCTAATTTTGTTGTGCAGAACA
AAGTTTTAAAAAAGAAGCGATTAATTTAATACTCGAACAAGGCTATACCGTTCAACAAGCGGCTAATGCACTCGGAATTACCACTAAATTGCTCTATGCT
TGGCGAAAGCGCCATGAAGCAGAAAATAAGCCTAACGCATTAACCCCTTTTGAGCGACAAGAGCTACTCGCTCTGCGCAAAGAAGTTAAGCAATTGAAAA
TGGAGAAAGAAATCCTAAAAAAGGCGAGAGTGCCTTCTTTGCGAAAGAGTTGCTATAAGGTGTTGGTTTGTTCAAGAGCATAAAACACAATATCCCATTA
AGCATTTATGTCGAGTGATGAAGCTCAGCCGTTCAACGTTTTATACTTGGCAAAAGCGCCCGGTTAAGCCACTTGATGATGACACGCTTGAATTGCATCA
AAAAGCTTGCTCCCTCTTTAGGGAAAGCCGGCAAAGCTTAGGTTATCGCATGTTAGCGGCTCAACTACGCAAAGAAGGTTATGCTATCAGTGATTATCGA
GCTCGAAAATTAATGAAGCAGTTAGGGTTAGTGGTTAAGCAAAGAAAACAATATCGAGTCACCTCGAAAAATAAAGCGACTGCGGATAATTTATTAAATC
AAAATTTTAATCCGGTAGCAGCGAATGAAGTGTGGGCTGGGGATATCACTTATTTAAAGACACCGGAAGGTTGGCTCTATCTGGCTATCGTGATGGATTT
ATATTCTCGGCGTATTGTGGGTTGGCACATGTCATCCAAAATAGATACTGCCTTGATAAGCAAAGCGTTAATGATGGCCTATAACTTACGTTCGCCCTCA
AAGGGTTGTGTGTTTCACAGTGACCGAGGAAGCCAATACACAAGTGGGCAATATCAAGCGTTACTGAAAAGTTATGATATGCGCTCAAGTCAAGGTGATA
TAGGGGCTTGCTGGGATAATGCGGTAGTTGAACGATTTTTTGGAAGTTTAAAACATGATTGGTTATTTAAAAGACCCCATGCAAATCGGTCAGAAATGAA
ACAAGACGTGCTGGATTATCTGCGTTATTACAATTTAACGCGATTACACACGGCAAATAATTATTTATCGCCTGTGGCGTACGAAAACTCCTTCAGAAAA
GTGTCCTAATTTTGTTGTGCAGAACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 315 bp | 104 aa | 69 | 383 | + | No |
Description : First part of the transposase
ORF sequence :
MKRKSPKQYTESFKKEAINLILEQGYTVQQAANALGITTKLLYAWRKRHEAENKPNALTPFERQELLALRKEVKQLKMEKEILKKARVPSLRKSCYKVLV
CSRA
CSRA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 891 bp | 296 aa | 319 | 1209 | + | No |
Description : Second part of the transposase
ORF sequence :
KRRECLLCERVAIRCWFVQEHKTQYPIKHLCRVMKLSRSTFYTWQKRPVKPLDDDTLELHQKACSLFRESRQSLGYRMLAAQLRKEGYAISDYRARKLMK
QLGLVVKQRKQYRVTSKNKATADNLLNQNFNPVAANEVWAGDITYLKTPEGWLYLAIVMDLYSRRIVGWHMSSKIDTALISKALMMAYNLRSPSKGCVFH
SDRGSQYTSGQYQALLKSYDMRSSQGDIGACWDNAVVERFFGSLKHDWLFKRPHANRSEMKQDVLDYLRYYNLTRLHTANNYLSPVAYENSFRKVS
QLGLVVKQRKQYRVTSKNKATADNLLNQNFNPVAANEVWAGDITYLKTPEGWLYLAIVMDLYSRRIVGWHMSSKIDTALISKALMMAYNLRSPSKGCVFH
SDRGSQYTSGQYQALLKSYDMRSSQGDIGACWDNAVVERFFGSLKHDWLFKRPHANRSEMKQDVLDYLRYYNLTRLHTANNYLSPVAYENSFRKVS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1141 bp | 380 aa | 69 | 1209 | + | Yes |
Chemistry : DDE
ORF sequence :
MKRKSPKQYTESFKKEAINLILEQGYTVQQAANALGITTKLLYAWRKRHEAENKPNALTPFERQELLALRKEVKQLKMEKEILKKRRECLLCERVAIRCW
FVQEHKTQYPIKHLCRVMKLSRSTFYTWQKRPVKPLDDDTLELHQKACSLFRESRQSLGYRMLAAQLRKEGYAISDYRARKLMKQLGLVVKQRKQYRVTS
KNKATADNLLNQNFNPVAANEVWAGDITYLKTPEGWLYLAIVMDLYSRRIVGWHMSSKIDTALISKALMMAYNLRSPSKGCVFHSDRGSQYTSGQYQALL
KSYDMRSSQGDIGACWDNAVVERFFGSLKHDWLFKRPHANRSEMKQDVLDYLRYYNLTRLHTANNYLSPVAYENSFRKVS
FVQEHKTQYPIKHLCRVMKLSRSTFYTWQKRPVKPLDDDTLELHQKACSLFRESRQSLGYRMLAAQLRKEGYAISDYRARKLMKQLGLVVKQRKQYRVTS
KNKATADNLLNQNFNPVAANEVWAGDITYLKTPEGWLYLAIVMDLYSRRIVGWHMSSKIDTALISKALMMAYNLRSPSKGCVFHSDRGSQYTSGQYQALL
KSYDMRSSQGDIGACWDNAVVERFFGSLKHDWLFKRPHANRSEMKQDVLDYLRYYNLTRLHTANNYLSPVAYENSFRKVS
Blast result :
Comments
ISKpn52 is 75% aa similar to ISSde6.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
There is an IS30 copy inserted at a site within the second ORF, these insertion was in silico deleted.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
There is an IS30 copy inserted at a site within the second ORF, these insertion was in silico deleted.
References
1] Qiaoxiang Cheng (2019) Direct submission.