ISKpn53
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Klebsiella pneumoniae | Klebsiella pneumoniae Escherichia coli UPEC132 |
DNA section
IS Length : 946 bp
Ends
IR Length : 31/42
IRL : TGTAAGTATCCCGCATAATCGTGCCATTCACATTTAGAGATCATCCGGCA
IRR : TGTAAATAGACCCATTTTAGTTCCATACACTTTTTGAGATCCCGGCCAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGTAAGTATC | TCTATTTACA | 0 | |
| CCGTCGGTGG | CTGCTGCTAC | 0 |
DNA sequence
TGTAAGTATCCCGCATAATCGTGCCATTCACATTTAGAGATCATCCGGCATAATCAATCTGCCAACGAAGGAGAGCGCTATGCGTAAAGCCCGTTTTACT
GAGCATCAGATCATCGCCGTGATTAAGTCGGTTGAAGCCGGACGAACTGTTAAAGATGTCTGTCGCGAGGCCGGAATCTCTGAAGCCACCTATTACAACT
GGAAGTCCAAATACGGCGGCATGGAGGCTTCTGATATTAAGAAAATCAAGGATCTTGAGGACGAGAACCGCCGTCTAAAACAAATGTTTGCCGACCTGAG
TCTGGAGAACCGGGCGCTGAAAGACGTTATCGAAAAAAAGCTTTAAAACCAGCCTTTAAGCGTGAGCTGGTTACTCATCTGATAACGACGTTCGAACTCA
GTATCCGTCAGGCCTGCCGGAGTCTGAACCTGAGCTGGTCTGTCGATTTTATGCATGATGCCCTGGTCTGTGGCCGTCGTTTTCGCATGTTCAATGTCGT
TGATGATTTTAACCGTGAGGCATTGTCGATTGAAATCGATCTGAATCTGCCAGCTCAGCGAGTGGTCCGTGTACTCGACAGGATCGCGGCAAACCGTGGC
TATCCGGCCATGCTTCGTCTGGATAACGGCCCGGAATTTATCTCCCTGGCACTGGCTGAATGGGCAGAGAAACATGCAATAAAACTGGAGTTTATCCAGC
CGGGTAAGCCGACGCAGAACGCTTTCATTGAGCGCTTTAACCGGACATACCGAACAGAAATACTCGATTTTTATCTGTTCAGAACGCTGAATGAAGTACG
GGAAATCACTGAAAAATGGTTATCAAAATATAACTGTGAACGCCCGCATGAATCGCTGAATAATATGACACCGGAGGAATACCGACAGCGCCATTATTTG
GCCGGGATCTCAAAAAGTGTATGGAACTAAAATGGGTCTATTTACA
GAGCATCAGATCATCGCCGTGATTAAGTCGGTTGAAGCCGGACGAACTGTTAAAGATGTCTGTCGCGAGGCCGGAATCTCTGAAGCCACCTATTACAACT
GGAAGTCCAAATACGGCGGCATGGAGGCTTCTGATATTAAGAAAATCAAGGATCTTGAGGACGAGAACCGCCGTCTAAAACAAATGTTTGCCGACCTGAG
TCTGGAGAACCGGGCGCTGAAAGACGTTATCGAAAAAAAGCTTTAAAACCAGCCTTTAAGCGTGAGCTGGTTACTCATCTGATAACGACGTTCGAACTCA
GTATCCGTCAGGCCTGCCGGAGTCTGAACCTGAGCTGGTCTGTCGATTTTATGCATGATGCCCTGGTCTGTGGCCGTCGTTTTCGCATGTTCAATGTCGT
TGATGATTTTAACCGTGAGGCATTGTCGATTGAAATCGATCTGAATCTGCCAGCTCAGCGAGTGGTCCGTGTACTCGACAGGATCGCGGCAAACCGTGGC
TATCCGGCCATGCTTCGTCTGGATAACGGCCCGGAATTTATCTCCCTGGCACTGGCTGAATGGGCAGAGAAACATGCAATAAAACTGGAGTTTATCCAGC
CGGGTAAGCCGACGCAGAACGCTTTCATTGAGCGCTTTAACCGGACATACCGAACAGAAATACTCGATTTTTATCTGTTCAGAACGCTGAATGAAGTACG
GGAAATCACTGAAAAATGGTTATCAAAATATAACTGTGAACGCCCGCATGAATCGCTGAATAATATGACACCGGAGGAATACCGACAGCGCCATTATTTG
GCCGGGATCTCAAAAAGTGTATGGAACTAAAATGGGTCTATTTACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 80 | 346 | + | No |
Description : First part of the transposase
ORF sequence :
MRKARFTEHQIIAVIKSVEAGRTVKDVCREAGISEATYYNWKSKYGGMEASDIKKIKDLEDENRRLKQMFADLSLENRALKDVIEKKL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 672 bp | 223 aa | 259 | 930 | + | No |
Description : Second part of the transposase
ORF sequence :
GREPPSKTNVCRPESGEPGAERRYRKKALKPAFKRELVTHLITTFELSIRQACRSLNLSWSVDFMHDALVCGRRFRMFNVVDDFNREALSIEIDLNLPAQ
RVVRVLDRIAANRGYPAMLRLDNGPEFISLALAEWAEKHAIKLEFIQPGKPTQNAFIERFNRTYRTEILDFYLFRTLNEVREITEKWLSKYNCERPHESL
NNMTPEEYRQRHYLAGISKSVWN
RVVRVLDRIAANRGYPAMLRLDNGPEFISLALAEWAEKHAIKLEFIQPGKPTQNAFIERFNRTYRTEILDFYLFRTLNEVREITEKWLSKYNCERPHESL
NNMTPEEYRQRHYLAGISKSVWN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 851 bp | 283 aa | 80 | 930 | + | Yes |
Chemistry : DDE
ORF sequence :
MRKARFTEHQIIAVIKSVEAGRTVKDVCREAGISEATYYNWKSKYGGMEASDIKKIKDLEDENRRLKQMFADLSLENRALKDVIEKKALKPAFKRELVTH
LITTFELSIRQACRSLNLSWSVDFMHDALVCGRRFRMFNVVDDFNREALSIEIDLNLPAQRVVRVLDRIAANRGYPAMLRLDNGPEFISLALAEWAEKHA
IKLEFIQPGKPTQNAFIERFNRTYRTEILDFYLFRTLNEVREITEKWLSKYNCERPHESLNNMTPEEYRQRHYLAGISKSVWN
LITTFELSIRQACRSLNLSWSVDFMHDALVCGRRFRMFNVVDDFNREALSIEIDLNLPAQRVVRVLDRIAANRGYPAMLRLDNGPEFISLALAEWAEKHA
IKLEFIQPGKPTQNAFIERFNRTYRTEILDFYLFRTLNEVREITEKWLSKYNCERPHESLNNMTPEEYRQRHYLAGISKSVWN
Blast result :
Comments
ISKpn53 is 96% aa similar to ISEcl10.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
There is a potential internal deletion in the ORFB and ORFAB.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
There is a potential internal deletion in the ORFB and ORFAB.
References
1] Zhu Zhichen (2019) Direct submission.