ISKpn54
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP041024 | ND | Klebsiella pneumoniae | Klebsiella pneumoniae KP1692 pKP1692 Klebsiella variicola X39 |
DNA section
IS Length : 1211 bp
Ends
IR Length : 24/33
IRL : TGATCTCCTCCCTGAAAACAGTGCCAGGCTAAACTGAAGTCTCCGGTCTT
IRR : TGACCTTGGCCCAAAATCAGTGCAGTGCTAAATCAGAGATCTTCGTCTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGATCTCCTC | GCCAGGGTCA | 0 | |
| AGCCACCTTTACCAAAGATC | AAGTTAAAAAGGAGCACACT | 0 | |
| GTAGAGTGAT | GAAAGACGAA | 0 |
DNA sequence
TGATCTCCTCCCTGAAAACAGTGCCAGGCTAAACTGAAGTCTCCGGTCTTTCTTCCATCTCACAGAGAGGCACATCGCCATGAAAAAGACCCGCTATACC
GAAGAACAGATAGCATTTGCGCTGAAACAGGCCGAAACCGGCACTCGGGTGGAGGAAGTCTGCAGAAAGATGGGCATTTCTGAAGCCACATTTTACAACT
GGAAGAAAAAGTTTGGCGGCATGGGCGTGACAGAGCTGCGCCGCCTGCGCCAGCTTGAGGAAGAGAATCAGCGTCTGAAACGTCTGGTGGCTGATTTAAG
TCTGGACAAGGAGATGCTGCAGGAAGTTATCAAAAAAAAGTTCTGAGGCCGGCTCAGAAGCGTCAGGCGGTGCATTTTCTGCGGGAGGCTTACCGTATCA
GTGTCCGGCGGGGATGCGGACTGCTGATGCAGAGCAGAACCGTTTACCACTGGCAGAGCCGGCGGGATGATCGGGCGATAACCCTGCGTATCCGGGAAAT
AGCGGAAACCCGGATCCGCTACGGTTGCCCGCGTATTCATATTCAGCTGCGGCGGGAAGGCTGGCCTGTTAACCACAAGAAAACACACCGGATTTACTGT
CTGGAAGGCCTCAACCTGCGCAGAAAGCGCCCCCGCAGGCATGTCAGTGCAGCACATCGTCAGCAACGTCCGGTCCTGACCGGTGTCGATCAGTGCTGGA
GTATGGATTTCGTGTCGGATAACCTGTTTAACGGACGACGTTTCCGGGCGCTGACTGTAGTGGATAATTTTAGTCGGGAGTGTGTGGCGATCCATGCCGG
AAAGTCGCTGAAAGGTGAGGATGTGGTCGGTGTGATGGAAAGGTTGCGGGTGCTGGGCAAGCGCCTGCCGGTACGTATTCAGACAGATAACGGCAGCGAG
TTTATCTCGAAAAGTCTGGATAAATGGGCGTATGAGCACGGTGTCACAATGGACTTCTCCCGCCCCGGAAAACCAACGGATAACCCGTTTATTGAATCAT
TTAACGGCAGTCTGCGGGATGAATGCCTGAACATTCACTGGTTTCTCTCGCTGGAAGATGCGCAGGACAAACTCGACAACTGGCGCAGGGAATATAATCA
TGAGAGAACGCATTCATCATTAAATGACATGACTCCGGCTGAATTCATCCGAAGTCTCCGGAAAGACGAAGATCTCTGATTTAGCACTGCACTGATTTTG
GGCCAAGGTCA
GAAGAACAGATAGCATTTGCGCTGAAACAGGCCGAAACCGGCACTCGGGTGGAGGAAGTCTGCAGAAAGATGGGCATTTCTGAAGCCACATTTTACAACT
GGAAGAAAAAGTTTGGCGGCATGGGCGTGACAGAGCTGCGCCGCCTGCGCCAGCTTGAGGAAGAGAATCAGCGTCTGAAACGTCTGGTGGCTGATTTAAG
TCTGGACAAGGAGATGCTGCAGGAAGTTATCAAAAAAAAGTTCTGAGGCCGGCTCAGAAGCGTCAGGCGGTGCATTTTCTGCGGGAGGCTTACCGTATCA
GTGTCCGGCGGGGATGCGGACTGCTGATGCAGAGCAGAACCGTTTACCACTGGCAGAGCCGGCGGGATGATCGGGCGATAACCCTGCGTATCCGGGAAAT
AGCGGAAACCCGGATCCGCTACGGTTGCCCGCGTATTCATATTCAGCTGCGGCGGGAAGGCTGGCCTGTTAACCACAAGAAAACACACCGGATTTACTGT
CTGGAAGGCCTCAACCTGCGCAGAAAGCGCCCCCGCAGGCATGTCAGTGCAGCACATCGTCAGCAACGTCCGGTCCTGACCGGTGTCGATCAGTGCTGGA
GTATGGATTTCGTGTCGGATAACCTGTTTAACGGACGACGTTTCCGGGCGCTGACTGTAGTGGATAATTTTAGTCGGGAGTGTGTGGCGATCCATGCCGG
AAAGTCGCTGAAAGGTGAGGATGTGGTCGGTGTGATGGAAAGGTTGCGGGTGCTGGGCAAGCGCCTGCCGGTACGTATTCAGACAGATAACGGCAGCGAG
TTTATCTCGAAAAGTCTGGATAAATGGGCGTATGAGCACGGTGTCACAATGGACTTCTCCCGCCCCGGAAAACCAACGGATAACCCGTTTATTGAATCAT
TTAACGGCAGTCTGCGGGATGAATGCCTGAACATTCACTGGTTTCTCTCGCTGGAAGATGCGCAGGACAAACTCGACAACTGGCGCAGGGAATATAATCA
TGAGAGAACGCATTCATCATTAAATGACATGACTCCGGCTGAATTCATCCGAAGTCTCCGGAAAGACGAAGATCTCTGATTTAGCACTGCACTGATTTTG
GGCCAAGGTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 80 | 346 | + | No |
Description : First part of the transposase
ORF sequence :
MKKTRYTEEQIAFALKQAETGTRVEEVCRKMGISEATFYNWKKKFGGMGVTELRRLRQLEEENQRLKRLVADLSLDKEMLQEVIKKKF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 885 bp | 294 aa | 295 | 1179 | + | No |
Description : Second part of the transposase
ORF sequence :
FKSGQGDAAGSYQKKVLRPAQKRQAVHFLREAYRISVRRGCGLLMQSRTVYHWQSRRDDRAITLRIREIAETRIRYGCPRIHIQLRREGWPVNHKKTHRI
YCLEGLNLRRKRPRRHVSAAHRQQRPVLTGVDQCWSMDFVSDNLFNGRRFRALTVVDNFSRECVAIHAGKSLKGEDVVGVMERLRVLGKRLPVRIQTDNG
SEFISKSLDKWAYEHGVTMDFSRPGKPTDNPFIESFNGSLRDECLNIHWFLSLEDAQDKLDNWRREYNHERTHSSLNDMTPAEFIRSLRKDEDL
YCLEGLNLRRKRPRRHVSAAHRQQRPVLTGVDQCWSMDFVSDNLFNGRRFRALTVVDNFSRECVAIHAGKSLKGEDVVGVMERLRVLGKRLPVRIQTDNG
SEFISKSLDKWAYEHGVTMDFSRPGKPTDNPFIESFNGSLRDECLNIHWFLSLEDAQDKLDNWRREYNHERTHSSLNDMTPAEFIRSLRKDEDL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1100 bp | 366 aa | 80 | 1179 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKTRYTEEQIAFALKQAETGTRVEEVCRKMGISEATFYNWKKKFGGMGVTELRRLRQLEEENQRLKRLVADLSLDKEMLQEVIKKKVLRPAQKRQAVHF
LREAYRISVRRGCGLLMQSRTVYHWQSRRDDRAITLRIREIAETRIRYGCPRIHIQLRREGWPVNHKKTHRIYCLEGLNLRRKRPRRHVSAAHRQQRPVL
TGVDQCWSMDFVSDNLFNGRRFRALTVVDNFSRECVAIHAGKSLKGEDVVGVMERLRVLGKRLPVRIQTDNGSEFISKSLDKWAYEHGVTMDFSRPGKPT
DNPFIESFNGSLRDECLNIHWFLSLEDAQDKLDNWRREYNHERTHSSLNDMTPAEFIRSLRKDEDL
LREAYRISVRRGCGLLMQSRTVYHWQSRRDDRAITLRIREIAETRIRYGCPRIHIQLRREGWPVNHKKTHRIYCLEGLNLRRKRPRRHVSAAHRQQRPVL
TGVDQCWSMDFVSDNLFNGRRFRALTVVDNFSRECVAIHAGKSLKGEDVVGVMERLRVLGKRLPVRIQTDNGSEFISKSLDKWAYEHGVTMDFSRPGKPT
DNPFIESFNGSLRDECLNIHWFLSLEDAQDKLDNWRREYNHERTHSSLNDMTPAEFIRSLRKDEDL
Blast result :
Comments
ISKpn54 is 97% aa similar to ISEch12
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Dongsheng Zhou (2019) Direct submission.
2] Lin,Y.-T. (2019) Direct GenBank submission.
2] Lin,Y.-T. (2019) Direct GenBank submission.