ISKpn64
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP024529 | ND | Klebsiella pneumoniae | Klebsiella pneumoniae Klebsiella pneumoniae strain INF157 plasmid INF157 p1 Klebsiella pneumoniae strain INF158 plasmid INF158 p1 Klebsiella pneumoniae strain KSB1_5D plasmid KSB1_5D p1 Klebsiella pneumoniae strain INF164 plasmid INF164 p1 Klebsiella pneumoniae strain INF163 plasmid INF163 p1 Klebsiella pneumoniae strain INF274 plasmid INF274 p1 Klebsiella pneumoniae strain INF278 plasmid INF278 p1 Klebsiella pneumoniae strain KSB1_9D plasmid KSB1_9D p1 |
DNA section
IS Length : 2396 bp
Ends
IR Length : 22/28
IRL : TGTTGATCAGCGCGCAAAAATGACCAGGTTGCCGGGGTTTTCAGCGTCCA
IRR : TGTCAATCAGCATCGAAAAATGACCAGGGATCAGCGTCCAAAATTGACCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCAACAAACCGAG | ATGTGCC | CATTCTGTATCGGGACT | 7 |
DNA sequence
TGTTGATCAGCGCGCAAAAATGACCAGGTTGCCGGGGTTTTCAGCGTCCAATTTTGACCACCCCGGAGTGTGTGCAACCATCCGGTTTTTGGACCGGAGA
ACCTGGGGTGTTAATCATGGAAACCGTGCTGAAAATACGGCGCATGCATCAGGTGGACGGCCAGTCCATCAGTGCCATCGCCAAAGTCACAGGCCTCTCT
CGCAATACCGTTCGCAAGTACCTTCGCCAACCTCCGTCCGAGCCGCCCAAGTACCGCCGCCAGCAGCCTGCCAAGCCTAAGTTGGGCGAGTTCGAGCCGG
CTCTCACCCAGTGGCTGGACCTCGATGCCCGGCGCCCCAAGCGCCAACGCCGCTGCGCCAAGCAGCTCTTCGAAGACCTGCAGCGCCTGGGCTACCAAGG
CGCCTACGACAGCGTGCAGCGCTTCGTCCGTGACCACAAAAGGCAGCCCGCCAAGACGGCAGCCTTTGTTCCCCTGACGTTCGCGGCCGGCGAGGCCTAC
CAGTTTGACTGGAGCGAGGAGGAGGTAGAGGTTGGTGGTGTGGTGCAACGGGTCAAAGCGGCCCACTTTCGTCTCACCCACAGCCGCCAGCCCTTTGTCA
TGGTGTTTCCCCGCGAGACCCAGGAGATGCTGTTTGCCGCCCATGATGCGGCCTTCCACTACTGGGGCGGCGTGCCGCGTCAGGGTATCTACGACAACCT
CAAGACCTGCGTCGACCAGGTGCTCGACGGCAAGGAGCGCCGCTTCAATCCCCGCTTCCTGGCCCTGATGAGCCATTATGTCATCGAGCCGGTGGCCTGC
ACCCCGGCCTCTGGCTGGGAGAAGGGCCAGGTCGAGAAACAGGTCCAGGATGTGCGCCGGCGCTGCTTCACCCCCAGACGCAAGGTAGACAGCCTGGCCG
AGCTCAACCGCCGGCTCATCCAGGAGTTGGAGCTGGCGGCACAGGCACGCCCGCATCCGGACGACCCGTCCCAAACGGTGTGGCAGCGCTTCGAGCTGGA
GCGGGCCGTCCTGCGCCCCCTGGCACCCCGCTTCAATGGCTATGTGGAGCGGGAGCTGCGCGTCGACAGCACCTGCCTCATCCACTTCGACCGCAACACC
TACAGTGTCGATAGCCGTTACGCCCATCGGCACGTGACCGCCAGGGTCTTCGCCGACCGACTCCAGGTGTACGCCCAGAGCGAGCTTATCGCCGACCATC
CTCGTCAATTTGGCCGGGACAAGACCCAGTACGACCCTTGGCACTACCTCGACCTGCTCGAGCACAAACCCGGCGCGCTGCGCCACGGCGCCCCCTTCAA
AGAGTGGACGCTGCCGCCGGCATTGAGCCGCTTGCAGCAGCAACTGATGGCTCGCAAGGGCGGTGATCGGGAGATGGTCAAGCTGCTGCTGGCCGCTAGG
CAGGATGGACTGGAGCTGCTGGAGCAGGCCTGCCGGCAGGTACTCCAACTGGGCGGAAGTTCCGCCGAGCTGGTGCTCAACCAACTGCAGCGGCTGCGCC
GCCCCGCCGAGGCGCCGGTGATCCATGCCCAGGTGGTGCCACTGAGCCGGCCGCCAGAGGCCAACTGCCAGCGCTATGACCGCCTGTTGCCGGGAGGTCA
GCATGTCAGCCGATGAGATCTACCAACGCCTGCAAGGGCTGAAACTGTTTGGCATGGCTGCTTTGTGCGACGAGCTGCTGGGGCATAGGGAGCGGCCCCT
GCCGCCGCCGGAGCAGTGGCTCAGACGGATGATCGAAGCCGAAGAGTCCGAGCGCCAATGCCGCTCGATCCGCTACCAGATGGGCATCGCCAAGTTCCCG
CTGGCCCGGGATCTGGACAGCTTCGACACCCGCGACTCACCGGTCAGCCCCGAGCAGCTGCGTGCGCTGGATCGGGATGACTTCCTAGCGCAGAAGCGCA
ACCTATTGCTGATCGGCGGGACCGGCACGGGGAAAACTCACCTGGCCATCGCCTTGGGGCGCAACGCCGTGCGCCGGGGCAAGCGGGTGCGCTTCTACGG
CGTGGTGGATCTGGTCAACCAACTGGAGCAGGAGAAGGCGGCCGGCAAAGCCGGACAACTGGCCAACCGGCTGGTCAATGTGGATGCCGTGATCCTGGAC
GAGCTGGGCTATCTGCCGTTTGCCGAAAGCGGTGGCGCCCTGCTGTTTCATCTCATCAGCAAGCTGTACGAGCGCACCAGCCTGATCCTGACCACCAACC
TGACCTTCAGCGAATGGGGCAAGGTGTTTACGGACGAGAAGATGACCACGGCGATGCTGGATCGGATCACTCATCACTGCGAGATCATCGAGACCGGAAA
CGACTCCTACCGCTTCAAACAGCGGATGAAATCAGCCAGCTAACCCTGGTCAATTTTGGACGCTGATCCCTGGTCATTTTTCGATGCTGATTGACA
ACCTGGGGTGTTAATCATGGAAACCGTGCTGAAAATACGGCGCATGCATCAGGTGGACGGCCAGTCCATCAGTGCCATCGCCAAAGTCACAGGCCTCTCT
CGCAATACCGTTCGCAAGTACCTTCGCCAACCTCCGTCCGAGCCGCCCAAGTACCGCCGCCAGCAGCCTGCCAAGCCTAAGTTGGGCGAGTTCGAGCCGG
CTCTCACCCAGTGGCTGGACCTCGATGCCCGGCGCCCCAAGCGCCAACGCCGCTGCGCCAAGCAGCTCTTCGAAGACCTGCAGCGCCTGGGCTACCAAGG
CGCCTACGACAGCGTGCAGCGCTTCGTCCGTGACCACAAAAGGCAGCCCGCCAAGACGGCAGCCTTTGTTCCCCTGACGTTCGCGGCCGGCGAGGCCTAC
CAGTTTGACTGGAGCGAGGAGGAGGTAGAGGTTGGTGGTGTGGTGCAACGGGTCAAAGCGGCCCACTTTCGTCTCACCCACAGCCGCCAGCCCTTTGTCA
TGGTGTTTCCCCGCGAGACCCAGGAGATGCTGTTTGCCGCCCATGATGCGGCCTTCCACTACTGGGGCGGCGTGCCGCGTCAGGGTATCTACGACAACCT
CAAGACCTGCGTCGACCAGGTGCTCGACGGCAAGGAGCGCCGCTTCAATCCCCGCTTCCTGGCCCTGATGAGCCATTATGTCATCGAGCCGGTGGCCTGC
ACCCCGGCCTCTGGCTGGGAGAAGGGCCAGGTCGAGAAACAGGTCCAGGATGTGCGCCGGCGCTGCTTCACCCCCAGACGCAAGGTAGACAGCCTGGCCG
AGCTCAACCGCCGGCTCATCCAGGAGTTGGAGCTGGCGGCACAGGCACGCCCGCATCCGGACGACCCGTCCCAAACGGTGTGGCAGCGCTTCGAGCTGGA
GCGGGCCGTCCTGCGCCCCCTGGCACCCCGCTTCAATGGCTATGTGGAGCGGGAGCTGCGCGTCGACAGCACCTGCCTCATCCACTTCGACCGCAACACC
TACAGTGTCGATAGCCGTTACGCCCATCGGCACGTGACCGCCAGGGTCTTCGCCGACCGACTCCAGGTGTACGCCCAGAGCGAGCTTATCGCCGACCATC
CTCGTCAATTTGGCCGGGACAAGACCCAGTACGACCCTTGGCACTACCTCGACCTGCTCGAGCACAAACCCGGCGCGCTGCGCCACGGCGCCCCCTTCAA
AGAGTGGACGCTGCCGCCGGCATTGAGCCGCTTGCAGCAGCAACTGATGGCTCGCAAGGGCGGTGATCGGGAGATGGTCAAGCTGCTGCTGGCCGCTAGG
CAGGATGGACTGGAGCTGCTGGAGCAGGCCTGCCGGCAGGTACTCCAACTGGGCGGAAGTTCCGCCGAGCTGGTGCTCAACCAACTGCAGCGGCTGCGCC
GCCCCGCCGAGGCGCCGGTGATCCATGCCCAGGTGGTGCCACTGAGCCGGCCGCCAGAGGCCAACTGCCAGCGCTATGACCGCCTGTTGCCGGGAGGTCA
GCATGTCAGCCGATGAGATCTACCAACGCCTGCAAGGGCTGAAACTGTTTGGCATGGCTGCTTTGTGCGACGAGCTGCTGGGGCATAGGGAGCGGCCCCT
GCCGCCGCCGGAGCAGTGGCTCAGACGGATGATCGAAGCCGAAGAGTCCGAGCGCCAATGCCGCTCGATCCGCTACCAGATGGGCATCGCCAAGTTCCCG
CTGGCCCGGGATCTGGACAGCTTCGACACCCGCGACTCACCGGTCAGCCCCGAGCAGCTGCGTGCGCTGGATCGGGATGACTTCCTAGCGCAGAAGCGCA
ACCTATTGCTGATCGGCGGGACCGGCACGGGGAAAACTCACCTGGCCATCGCCTTGGGGCGCAACGCCGTGCGCCGGGGCAAGCGGGTGCGCTTCTACGG
CGTGGTGGATCTGGTCAACCAACTGGAGCAGGAGAAGGCGGCCGGCAAAGCCGGACAACTGGCCAACCGGCTGGTCAATGTGGATGCCGTGATCCTGGAC
GAGCTGGGCTATCTGCCGTTTGCCGAAAGCGGTGGCGCCCTGCTGTTTCATCTCATCAGCAAGCTGTACGAGCGCACCAGCCTGATCCTGACCACCAACC
TGACCTTCAGCGAATGGGGCAAGGTGTTTACGGACGAGAAGATGACCACGGCGATGCTGGATCGGATCACTCATCACTGCGAGATCATCGAGACCGGAAA
CGACTCCTACCGCTTCAAACAGCGGATGAAATCAGCCAGCTAACCCTGGTCAATTTTGGACGCTGATCCCTGGTCATTTTTCGATGCTGATTGACA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1500 bp | 499 aa | 117 | 1616 | + | No |
Chemistry : DDE
ORF sequence :
METVLKIRRMHQVDGQSISAIAKVTGLSRNTVRKYLRQPPSEPPKYRRQQPAKPKLGEFEPALTQWLDLDARRPKRQRRCAKQLFEDLQRLGYQGAYDSV
QRFVRDHKRQPAKTAAFVPLTFAAGEAYQFDWSEEEVEVGGVVQRVKAAHFRLTHSRQPFVMVFPRETQEMLFAAHDAAFHYWGGVPRQGIYDNLKTCVD
QVLDGKERRFNPRFLALMSHYVIEPVACTPASGWEKGQVEKQVQDVRRRCFTPRRKVDSLAELNRRLIQELELAAQARPHPDDPSQTVWQRFELERAVLR
PLAPRFNGYVERELRVDSTCLIHFDRNTYSVDSRYAHRHVTARVFADRLQVYAQSELIADHPRQFGRDKTQYDPWHYLDLLEHKPGALRHGAPFKEWTLP
PALSRLQQQLMARKGGDREMVKLLLAARQDGLELLEQACRQVLQLGGSSAELVLNQLQRLRRPAEAPVIHAQVVPLSRPPEANCQRYDRLLPGGQHVSR
QRFVRDHKRQPAKTAAFVPLTFAAGEAYQFDWSEEEVEVGGVVQRVKAAHFRLTHSRQPFVMVFPRETQEMLFAAHDAAFHYWGGVPRQGIYDNLKTCVD
QVLDGKERRFNPRFLALMSHYVIEPVACTPASGWEKGQVEKQVQDVRRRCFTPRRKVDSLAELNRRLIQELELAAQARPHPDDPSQTVWQRFELERAVLR
PLAPRFNGYVERELRVDSTCLIHFDRNTYSVDSRYAHRHVTARVFADRLQVYAQSELIADHPRQFGRDKTQYDPWHYLDLLEHKPGALRHGAPFKEWTLP
PALSRLQQQLMARKGGDREMVKLLLAARQDGLELLEQACRQVLQLGGSSAELVLNQLQRLRRPAEAPVIHAQVVPLSRPPEANCQRYDRLLPGGQHVSR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 768 bp | 255 aa | 1576 | 2343 | + | No |
AG : IS21 helper
ORF sequence :
MTACCREVSMSADEIYQRLQGLKLFGMAALCDELLGHRERPLPPPEQWLRRMIEAEESERQCRSIRYQMGIAKFPLARDLDSFDTRDSPVSPEQLRALDR
DDFLAQKRNLLLIGGTGTGKTHLAIALGRNAVRRGKRVRFYGVVDLVNQLEQEKAAGKAGQLANRLVNVDAVILDELGYLPFAESGGALLFHLISKLYER
TSLILTTNLTFSEWGKVFTDEKMTTAMLDRITHHCEIIETGNDSYRFKQRMKSAS
DDFLAQKRNLLLIGGTGTGKTHLAIALGRNAVRRGKRVRFYGVVDLVNQLEQEKAAGKAGQLANRLVNVDAVILDELGYLPFAESGGALLFHLISKLYER
TSLILTTNLTFSEWGKVFTDEKMTTAMLDRITHHCEIIETGNDSYRFKQRMKSAS
Blast result :
Comments
ISKpn64 is 66% aa similar (transposase) to ISMdi27.
References
1] Ambrose SJ. and Hall RM. (2020) Direct submission