ISKpn71

  • Family IS256
  • Group IS1249
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Klebsiella pneumoniae
Klebsiella pneumoniae
DNA section
IS Length : 1103 bp

Ends


IR Length : 22/28

IRL : GGTCTAATAGACAAAATTCATGATGAAACCCCACTCAAACCCTGATACCA
IRR : GGCGTAATAGACATTTTTCATGATAAAAATCACACCCCATCTCCTAATAG

Insertion site


Left flankDirect repeatRight flankDR Length
GTATTTCTTGCAGCA AAAATAATCAAAAAAAATGTTG0
TTATTTATTAATTGAAAAATAAT CAAAACCGCAAGAT0

DNA sequence

GGTCTAATAGACAAAATTCATGATGAAACCCCACTCAAACCCTGATACCATATAGTTTTGAGTGGGGTTTATTGTTATGTTTAAAAACTTGCCCATTTTG
TCATTCTCTCAATACTGTCAAAAACGGTAAAAAACAGCATCAACAGCAGTATTATTGTAAATCCTGTCATCGTCAATTTATCGGAAAATCGCGATTATCC
AATGATCAGCTTTGGCTTGACTATACAGAAGGCAAGCAGACTTATAGGCAATTGGCTTTAAAATACAACTTATCAATCAGAACCATCCAAAGGCGATTAG
ATCGGGTTGATATTGCTCACAGTCATTCTCAAGTATCCCTACCTAGTGCCACCAATATCATTATGGACACCACCTACTTTGGTCGGGACTTTGGTGTGAT
GGTGTTTATGGATAATCAGACAGGCAAGGTACTGCTTTATCACTTTGTACACCACGAAACCAATGCTTTATACAAACAAGGCATTCAAAGATTGCAAGCA
CAAGGTATGGTTATTCACAGCATCACTTGTGATGGTCGTAGGGGATTGCTGGGTGGTTTTGATGCCATCTTGACACAGATGTGCCAATTTCATCAGCAAC
AAATCATCAGACGCTATGTCACAAAAAATCCTAAGCATCCTGCTGCAAAAGAATTGTTCGCACTGTGTCAAACATTGACACAGTGCAATGCAAAACAATT
TAAACAAGCATTGGATACATGGCTAATGCATCATTGTGACTATTTGAATGAGCGTTCTGTCAGTGAGGATGGTGCAAGAAGTTGGTATACCCACAAAAGA
CTAAGAAGTGCTTATAGAAGCTTGGTGAATCATTTGCCCTATTTGTTTGTTTATCACACCCATCCCAAACAAATTGCCAATACCACCAATAAACTCGAAG
GGCTGTTTAGTGATCTTAAGCAAAAGCTAAGAAATCATCAAGGATTAAACAAAACAAGAAAACAAAGATTTATCGAATCTTATTTAAAATCACACCAACT
CCATGAATGATAGTAACAGCAATATTCATGAACAACTGTATCATGAAAAATGTCTATTAGGAGATGGGGTGTGATTTTTATCATGAAAAATGTCTATTAC
GCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
939 bp332 aa721010+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLCLKTCPFCHSLNTVKNGKKQHQQQYYCKSCHRQFIGKSRLSNDQLWLDYTEGKQTYRQLALKYNLSIRTIQRRLDRVDIAHSHSQVSLPSATNIIMDT
TYFGRDFGVMVFMDNQTGKVLLYHFVHHETNALYKQGIQRLQAQGMVIHSITCDGRRGLLGGFDAILTQMCQFHQQQIIRRYVTKNPKHPAAKELFALCQ
TLTQCNAKQFKQALDTWLMHHCDYLNERSVSEDGARSWYTHKRLRSAYRSLVNHLPYLFVYHTHPKQIANTTNKLEGLFSDLKQKLRNHQGLNKTRKQRF
IESYLKSHQLHE

 

Blast result :
Comments
Two copies of ISKpn71 form a composite transposon, its insertion generate a direct repeat of 8 nts (AAAATAAT).Between the two copies, there are a phosphoethanolamine--lipid A transferase MCR-2.3, there is also an insertion of ISSpu2, an IS630 family member.
References
1] Milan Stosic (2020) direct sumission.