ISNarch5

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_CP050695.1 ND Natrialbaceae archaeon
Natrialbaceae archaeon XQ-INN 246 strain 2447
DNA section
IS Length : 2312 bp

Ends


IR Length : 17/24

IRL : GTAACCGCTCCACGAACCCCATCTGTTGAGCGGATTGTGGTTCCGGGACA
IRR : GTAAGCGATCCGGGAACTGCACCTACTCGGTGGGTTTGAGCGTGTATAGG

Insertion site


Left flankDirect repeatRight flankDR Length
CTGGAGCAGCGGAGCAGCGGAGCAGCGC8

DNA sequence

GTAACCGCTCCACGAACCCCATCTGTTGAGCGGATTGTGGTTCCGGGACACTGAGCCTTCCGGATTGATGGGAGGCTTGGAGCATGTGGTTATCCCCAGT
ACGCGCCCGCTGGCTGGGCTATCTGTATCACGCGCACGCCAGCGGCCTGACGTTGAGCGAGTATGCCGCCCGGCAGGATGTCTCGCTTGCCGAGCTGATG
GACTGGGAGCGCCGGCTGCGTGAGGCCGGGATTGCGGTGCCGGAGCGCCACCGCCCGGCACGGTTCGTCGCCGTGGAGGTGGTGGCATGATCCGGCCCGG
GAGCGATGTCGCTGTTTATCTGTGCCGCGAGCCCGTGGATATGCGTAAGTCGATGGACGGATTGTCTTTGCTCGTCCAGGAGGTCATGGAGTGCGACCCG
TTCACCGCGGCGGTGTTCGTGTTCTGCAACCGGGCGCGGGATAAGGTGAAGATCCTATTCTGGGAGCGCAACGGCTTCGTGGTCTGGTATAAGCGCCTCG
AGCAGGAGCGGTTCAAGTGGCCGGAGTGCGGCGAAGGGGATCGGCTCACGCTCTCGGGCCAGGAGCTCAACTGGCTGCTTGACGGCATCGATATCACCCG
CATGCAGCCGCACAAAGCGCTGCATTTTCAGTCGGTTGGATGAAATTTTTGCTCGCCGCACCGGTGTCGTTTTGGTACAATTGTCGGCATGAAACGGGCC
GATACCAACACGTTGCCATCCAGTTCCGACCTCCAGCGCGAGCTCGACGAGCAGCGCGCTCTGGTCGAACGCCTCCAGGCCCAGCTCGCCGAGAAGGAGG
CCGCGTGGGCGGCGGAGAAGCGCTCGCTGTTCGAGCAGATCCGGCTGCTGCTCGATAACCGCTTCGGCCCCTCCACCGAGAAGTACAGCATCAAGCAGCA
GGACATGTTCTTCGATGAGGCCGAGAGCCTGGTGGAAGAGCCCGCCGAGTCTGATGAGACCGACGAGGCGGATGAGGACAACCAGCCAGTCCGCGGTAAG
CGCCGCCGTCGGGGCGGCCGCGCCCCGCTGCCACCGGAGTTGCCCCGCGTGGACATCGTCCACGACCTCCCCGAGGACGAACAGCAGTGCGCCTGTGGCT
GCGGTGCGCTCACCCGCATCGGCGAAGAAGTCACCGAGCAGCTCGACATCATCCCGGCCCAGATCCAGGTGCTGCGCCATGTGCGCATCAAGTACGCCTG
CCGGGCCTGCGAAGACGGCGTCCAGATCGCCGATCTGCCACCGCAGCCGCTGCCAAAGAGCAACGCGAGCCCCGGGCTGCTCGCTTATATCGCCACCGCC
AAGTACCAGGATGCGCTGCCACTGTACCGCCAGGAGCAGGTGTTCAAACGACTGGGCCTGGAGTTGCCACGGAACACGCTCGCCCGCTGGATGGTAGACA
TGGGCGCGCTGCTCGCCCCACTGGCCGAGCGCATGCGCGCCCATCTGCACAATGCGGAACTCATCCACATGGACGAGACCACCGTGCAGGTGAACACCGA
GCCCGGGCGGGCCGCCTCCAGCACCTCGTACATGTGGGTCCAGCGCGGCGGACCACCCGGTGCCGAGGTGGTGCTGTTCGACTACGATCCCAGCCGCTCG
GGCCAGGTGCCGCGCCGTCTGCTGGACGACTACAACGGCATCCTGCTCTCTGATGGCTACGAGGGCTATGCCCAGGTGGTGCGCGACAATGCGATCACTC
ACGCTGGCTGCTGGGCGCATGCGCGCCGCAAGTTCGTTGAGGCCCAGAAAGCCCAGCCCAAGGGCAAGACCGGCAAGGCCGACCGAGCTCTGGCGTCCAT
CGGCAAACTCTACCGTGTGGAGCGCGAGGCACAGGGTCTGCCCGTTGAGGAGCGTGAACGCCTGCGTGCCACGCACAGCCGGCCGCTGATCGAGGATCTG
CGCCAGTGGCTTGACCAGTCCCTGGAGAAGGTGCCGCCGAAGAGCGCCATCGGCAAGGCCGTGCACTACCTCAACAGCCAATGGCCCCGGCTCATCCGCT
TCCTGGAGGATGGCCGCATCCCGCTGGACAACAACCCTGCGGAGAACGCCATCCGGCCGTTCGTGGTGGGGCGCAAGAACTGGCTGTTCAGCCAGACGCC
GAGGGGTGCCCACGCCAGCGCAGCGATCTACAGCGTCATCGAGACGGCCAAGATCAACGGCCTGGAGCCCTACGCGTACCTGCTCGAGGTGTTAAAGAAC
CTGCCGGCGGCGGCCAGCGATGAGGCCATCGACGGCCTGCTGCCGTGGCATCAGGATGAGAGCCTATACACGCTCAAACCCACCGAGTAGGTGCAGTTCC
CGGATCGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
207 bp68 aa84290+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MWLSPVRARWLGYLYHAHASGLTLSEYAARQDVSLAELMDWERRLREAGIAVPERHRPARFVAVEVVA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
357 bp118 aa287643+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIRPGSDVAVYLCREPVDMRKSMDGLSLLVQEVMECDPFTAAVFVFCNRARDKVKILFWERNGFVVWYKRLEQERFKWPECGEGDRLTLSGQELNWLLDG
IDITRMQPHKALHFQSVG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1578 bp585 aa7132290+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

LPSSSDLQRELDEQRALVERLQAQLAEKEAAWAAEKRSLFEQIRLLLDNRFGPSTEKYSIKQQDMFFDEAESLVEEPAESDETDEADEDNQPVRGKRRRR
GGRAPLPPELPRVDIVHDLPEDEQQCACGCGALTRIGEEVTEQLDIIPAQIQVLRHVRIKYACRACEDGVQIADLPPQPLPKSNASPGLLAYIATAKYQD
ALPLYRQEQVFKRLGLELPRNTLARWMVDMGALLAPLAERMRAHLHNAELIHMDETTVQVNTEPGRAASSTSYMWVQRGGPPGAEVVLFDYDPSRSGQVP
RRLLDDYNGILLSDGYEGYAQVVRDNAITHAGCWAHARRKFVEAQKAQPKGKTGKADRALASIGKLYRVEREAQGLPVEERERLRATHSRPLIEDLRQWL
DQSLEKVPPKSAIGKAVHYLNSQWPRLIRFLEDGRIPLDNNPAENAIRPFVVGRKNWLFSQTPRGAHASAAIYSVIETAKINGLEPYAYLLEVLKNLPAA
ASDEAIDGLLPWHQDESLYTLKPTE

 

Blast result :Comments : 78% similar to ISAeh1 transposase
Comments
ISNarch5 is 97% aa (transposase) similar to ISNarch2.
References
1] Sarah Sonbol (2020) Direct submission.
2] Xue,Q. (2020) Direct GenBank submission.