ISKpn72

  • Family IS4
  • Group IS10
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
Y Klebsiella pneumoniae
Klebsiella pneumoniae
DNA section
IS Length : 1329 bp

Ends


IR Length : 17/22

IRL : CTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGAT
IRR : CTGAGAGATCCCCTCATAATTTCCCCAAAGCGTAACCATGTGTGAATAAA

Insertion site


Left flankDirect repeatRight flankDR Length
AAAGCGCCGAAGCCAGGTCGTGGTCGATC9
AACCCATTGCTGCTTTATGGTGATTGTGG9
GCGGCAATAATGCCAAGTGCGATTAATGG9
CTGAAGGGGCAGTTTAGCCTGCGTCACTA9
GGAAAAAAGATGTTAAAGATTCAAGATTT9
ATGCCACCAACGCCAGGCAGGTGGTGGAG9
CGGCGGCGCTGATTAAGCGTTTTTCCTAT9
CTTTATTACTGGCTTCGCAGCCAATATGC9
CTGGGGATGGAGCATAGCCCCAAACCGCT9
TACTGCTTCAATCATAGCGTCCTCCTGAC9
CGGTATTGCGAACAAAGCGTGAACTGGCG9
TTTAAATAGCCACTTAGCATTCGGCGTAA9

DNA sequence

CTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGA
CAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCAACAGCTTAACGTTGGCTTGCCAC
GCATTACTTGACTGTAAAACTCTCACTCTTACCGAACTTGGCCGTAACCTGCCAACCAAAGCGAGAACAAAACATAACATCAAACGAATCGACCGATTGT
TAGGTAATCGTCACCTCCACAAAGAGCGACTCGCTGTATACCGTTGGCATGCTAGCTTTATCTGTTCGGGCAATACGATGCCCATTGTACTTGTTGACTG
GTCTGATATCCGTGAGCAAAAACGGCTTATGGTATTGCGAGCTTCAGTCGCACTACACGGTCGTTCTGTTACTCTTTATGAGAAAGCGTTCCCGCTTTCA
GAGCAATGTTCAAAGAAAGCTCATGACCAATTTCTAGCCGACCTTGCGAGCATTCTACCGAGTAACACCACACCGCTCATTGTCAGTGATGCTGGCTTTA
AAGTGCCATGGTATAAATCCGTTGAGAAGCTGGGTTGGTACTGGTTAAGTCGAGTAAGAGGAAAAGTACAATATGCAGACCTAGGAGCGGAAAACTGGAA
ACCTATCAGCAACTTACATGATATGTCATCTAGTCACTCAAAGACTTTAGGCTATAAGAGGCTGACTAAAAGCAATCCAATCTCATGCCAAATTCTATTG
TATAAATCTCGCTCTAAAGGCCGAAAAAATCAGCGCTCGACACGGACTCATTGTCACCACCCGTCACCTAAAATCTACTCAGCGTCGGCAAAGGAGCCAT
GGGTTCTAGCAACTAACTTACCTGTTGAAATTCGAACACCCAAACAACTTGTTAATATCTATTCGAAGCGAATGCAGATTGAAGAAACCTTCCGAGACTT
GAAAAGTCCTGCCTACGGACTAGGCCTACGCCATAGCCGAACGAGCAGCTCAGAGCGTTTTGATATCATGCTGCTAATCGCCCTGATGCTTCAACTAACA
TGTTGGCTTGCGGGCGTTCATGCTCAGAAACAAGGTTGGGACAAGCACTTCCAGGGTAACACAGTCAGAAATCGAAACGTACTCTCAACAGTTCGCTTAG
GCATGGAAGTTTTGCGGCATTCTGGCTACACAATAACAAGGGAAGACTTACTCGTGGCTGCAACCCTACTAGCTCAAAATTTATTCACACATGGTTACGC
TTTGGGGAAATTATGAGGGGATCTCTCAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1209 bp402 aa1081316+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSD
IREQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPI
SNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEETFRDLKS
PAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQGNTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALG
KL

 

Blast result :
Comments
ISKpn72 is an isoform of IS10R (99% aa similarity).
This IS is observed to inactivate mgrB gene of hospital clinical strain Klebsiella pneumoniae R10-341 and result in colistin-resistance.
References
1] Yangbo Hu (2020) Direct submission.