ISKmi1

  • Family IS30
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Klebsiella michiganensis
Klebsiella michiganensis
Enterobacter hormaechei Y233 plasmid pIHI2-233
DNA section
IS Length : 1088 bp

Ends


IR Length : 23/29

IRL : CGCGAATTCAGATAATAACTGCAACACCCATGGTGCATAGGAGAGAAGAG
IRR : CGCGAATTCAACTCCGAAGTGCAACACCCGCCAAATCAAGCAACCTGTCG

Insertion site


Left flankDirect repeatRight flankDR Length
GATATCGTCAGACCAGTACTGA2

DNA sequence

CGCGAATTCAGATAATAACTGCAACACCCATGGTGCATAGGAGAGAAGAGGCCAACTGCCGGTTCTGGTACGCTTCTTCTCGCCAAAGAAACAAAGCGGA
ACACCATGAGATACCATCAGTTGACCGAAGGACAACGATACCAGATCGCCTGCCTTCATGACCATGGCTTGAGCCAAGCAATGATTGCCCGCAAGGTGGG
TGTGCATCCATCCACCATTAGTCGCGAACTCCGTCGCAACCGGTGTGCGCAAGGTTATCAACCAGCAGAGGCTCATCAATCGGCCACCCTGCGACGATGT
TCCAGCAGCAAATATCGTGTCCCGGAAGATACCCTTATTTTCGTGACCATGGCGTTGGCCGCAGACTGGAGTCCGGAGCAGATAAGCTCGGTCTGCAGGC
GCATTGGTTGCCCTGTCAGTCATGAGTGGATCTACCGCTATGTGGCGCAGGATAAGGCCAACGGTGGCAAGCTGTTCCGTCACTTGCGGCAAGGCCACAA
ACGTTACCGTAAGGGACAGAATGCCAAGCGCTGCGTGATCCCCAACCCGGTATCGATCGATGAGCGCCCAGCGATAGTCGACTCTCGTAGCCGATTGGGT
GACTGGGAGGTGGATACCGTTTCGGGCAAACAAGGTAGTGGTGCCATCGTGACACTGGCCGAGCGTAAGAGCCGACTCTACCTAGTGCAGTATGTGCCTG
CCAAGACGGCAGCTGCAGTGGCCGATGCCATCATCTGCATGCTAGAGCCCTACAAGGCACATGTTCATACAATCACCGCGGACAATGGCAGTGAATTTGT
GGAACATGAGCGAGTAGCCAAGGTGCTAAATGCCCAGTTTTACTTCGCGCATCCATACAGCGCGTGGGAGCGAGGTCTGAACGAGAACAGCAATGGCTTG
TTGCGGCAGTATGTGCCCAAGGGCAGCGACCTGAGCCAGCTCAGCCCGGATGAGTTGTCGGCCTTCGAGCGGCGACTCAACCTTAGACCGCGCAAATGTT
TGGATTTCAGGCAACCGCAGATCGTGTTTGATGAACTACGACAGGTTGCTTGATTTGGCGGGTGTTGCACTTCGGAGTTGAATTCGCG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
948 bp315 aa1061053+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRYHQLTEGQRYQIACLHDHGLSQAMIARKVGVHPSTISRELRRNRCAQGYQPAEAHQSATLRRCSSSKYRVPEDTLIFVTMALAADWSPEQISSVCRRI
GCPVSHEWIYRYVAQDKANGGKLFRHLRQGHKRYRKGQNAKRCVIPNPVSIDERPAIVDSRSRLGDWEVDTVSGKQGSGAIVTLAERKSRLYLVQYVPAK
TAAAVADAIICMLEPYKAHVHTITADNGSEFVEHERVAKVLNAQFYFAHPYSAWERGLNENSNGLLRQYVPKGSDLSQLSPDELSAFERRLNLRPRKCLD
FRQPQIVFDELRQVA

 

Blast result :
Comments
ISKmi1 is 86% aa similar to ISAeca1.
References
1] Shuangshuang Li (2021) Direct submission.