ISKmi2
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| LR607351 | ND | Klebsiella michiganensis | Klebsiella michiganensis 4928STDY7071171 |
DNA section
IS Length : 1331 bp
Ends
IR Length : 30/42
IRL : TAGACTGGCCCCCTGAATCTCCAGACAGTTGGTATCACTTAAGTTAGTGA
IRR : TGTATTTGACCCTATATTTCCAGACACCTGTTATCACTTAACCCATTACT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACCGAACTGC | AAAAG | CTCGATGTGA | 5 |
DNA sequence
TAGACTGGCCCCCTGAATCTCCAGACAGTTGGTATCACTTAAGTTAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCGTTGGAGTCCGGATGATTGAT
GTTTTAGGTCCAGAGAAGCGCAGACGTCGCAGCATTCAGGAAAAAATTGCCATTGTTCAGAAGAGCTTTGAACCCGGCATGACGGTGTCGCTGGTCGCCC
GTCAGCATGGTGTCGCTGCCAGTCAGTTGTTCCTGTGGCGCAAGCAGTACCAAGAAGGCAGCCTCATAGCCGTTGCCGCAGGAGAACAGGTTGTGCCCGC
GTCGGAACTGGCATCTGCGATGAAGCAAATCAAAGAGCTGCAGCGCCTGCTGGGCAAGAAAACCATGGAAAACGAGCTGCTGAAAGAAGCCGTTGAATAT
GGTCGACAAAAAAAGTGGATAGCGCACGTGCCCTTGTTGCCGGAGGATGGCGAATAAGCCTTGTCAGCCGTTGCCTCCGGGTGTCGCGTGCGCAGTTACA
CGTCATTCTCAGACGAACCGATGACTGGAAGGATGGGCGCCGCAGCCGTGGCACTGATGATACGGATGCGCTTGCCCGTATACACCATGTTATCGGAGAG
CTGCCCACGTATGGTTATCGCCGGGTGTGGGCTCTGCTCCGCAGACAGACCGAACTTGATGGTGCGGCAGTGATCAACGCCAAACGTGTTTACCGGATCA
TGCGCCTGAATGCACTGCTGCTTGAGCGTAAACCCGCCGTCCCCCCATCGAAACGGGCACATACCGGCAGAGTGGCCGTGAAGGAAAGCAACCAGCGGTG
GTGCTCTGACGGGTTCGAGTTCCGCTGTGATAACGGTGAAAAACTGCGTGTCACGTTCGCGCTGGACTGCTGCGATCGTGAAGCACTGCACTGGGCGGCC
AGCACGGGCGGCTTCGACAGTGAAACTGTACAGGACGTCATGCTGGGAGCGGTGGAGCGTCGCTTCGGCAGCGGCCTTCCGGCGTCGCCGGTGGAGTGGC
TGACGGATAACGGTTCCTGCTACCGGGCGTATGAAACACGGCAGTTCGCCCGGATCGTGGGTCTTGAACCGAAAAACACGGCTGTGCGGAGCCCGGAGAG
TAACGGAATAGCAGAGAGCTTCGTAAAAACGATAAAGCGTGACTACATCAGTATCATGCCTAAACCCGACGGGTTAACGGCAGTAAAGAACCTTGCGGAA
GCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCGGCGTACCAGTAATGGGTTAAGTGATA
ACAGGTGTCTGGAAATATAGGGTCAAATACA
GTTTTAGGTCCAGAGAAGCGCAGACGTCGCAGCATTCAGGAAAAAATTGCCATTGTTCAGAAGAGCTTTGAACCCGGCATGACGGTGTCGCTGGTCGCCC
GTCAGCATGGTGTCGCTGCCAGTCAGTTGTTCCTGTGGCGCAAGCAGTACCAAGAAGGCAGCCTCATAGCCGTTGCCGCAGGAGAACAGGTTGTGCCCGC
GTCGGAACTGGCATCTGCGATGAAGCAAATCAAAGAGCTGCAGCGCCTGCTGGGCAAGAAAACCATGGAAAACGAGCTGCTGAAAGAAGCCGTTGAATAT
GGTCGACAAAAAAAGTGGATAGCGCACGTGCCCTTGTTGCCGGAGGATGGCGAATAAGCCTTGTCAGCCGTTGCCTCCGGGTGTCGCGTGCGCAGTTACA
CGTCATTCTCAGACGAACCGATGACTGGAAGGATGGGCGCCGCAGCCGTGGCACTGATGATACGGATGCGCTTGCCCGTATACACCATGTTATCGGAGAG
CTGCCCACGTATGGTTATCGCCGGGTGTGGGCTCTGCTCCGCAGACAGACCGAACTTGATGGTGCGGCAGTGATCAACGCCAAACGTGTTTACCGGATCA
TGCGCCTGAATGCACTGCTGCTTGAGCGTAAACCCGCCGTCCCCCCATCGAAACGGGCACATACCGGCAGAGTGGCCGTGAAGGAAAGCAACCAGCGGTG
GTGCTCTGACGGGTTCGAGTTCCGCTGTGATAACGGTGAAAAACTGCGTGTCACGTTCGCGCTGGACTGCTGCGATCGTGAAGCACTGCACTGGGCGGCC
AGCACGGGCGGCTTCGACAGTGAAACTGTACAGGACGTCATGCTGGGAGCGGTGGAGCGTCGCTTCGGCAGCGGCCTTCCGGCGTCGCCGGTGGAGTGGC
TGACGGATAACGGTTCCTGCTACCGGGCGTATGAAACACGGCAGTTCGCCCGGATCGTGGGTCTTGAACCGAAAAACACGGCTGTGCGGAGCCCGGAGAG
TAACGGAATAGCAGAGAGCTTCGTAAAAACGATAAAGCGTGACTACATCAGTATCATGCCTAAACCCGACGGGTTAACGGCAGTAAAGAACCTTGCGGAA
GCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCGGCGTACCAGTAATGGGTTAAGTGATA
ACAGGTGTCTGGAAATATAGGGTCAAATACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 366 bp | 121 aa | 92 | 457 | + | No |
Description : First part of the transposase
ORF sequence :
MIDVLGPEKRRRRSIQEKIAIVQKSFEPGMTVSLVARQHGVAASQLFLWRKQYQEGSLIAVAAGEQVVPASELASAMKQIKELQRLLGKKTMENELLKEA
VEYGRQKKWIAHVPLLPEDGE
VEYGRQKKWIAHVPLLPEDGE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 397 | 1320 | + | No |
Description : Second part of the transposase
ORF sequence :
IWSTKKVDSARALVAGGWRISLVSRCLRVSRAQLHVILRRTDDWKDGRRSRGTDDTDALARIHHVIGELPTYGYRRVWALLRRQTELDGAAVINAKRVYR
IMRLNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAASTGGFDSETVQDVMLGAVERRFGSGLPASPVE
WLTDNGSCYRAYETRQFARIVGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAVKNLAEAFEHYNEWHPHSALGYRSPREYLRRRTSNGLS
DNRCLEI
IMRLNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAASTGGFDSETVQDVMLGAVERRFGSGLPASPVE
WLTDNGSCYRAYETRQFARIVGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAVKNLAEAFEHYNEWHPHSALGYRSPREYLRRRTSNGLS
DNRCLEI
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1229 bp | 409 aa | 92 | 1320 | + | Yes |
Chemistry : DDE
ORF sequence :
MIDVLGPEKRRRRSIQEKIAIVQKSFEPGMTVSLVARQHGVAASQLFLWRKQYQEGSLIAVAAGEQVVPASELASAMKQIKELQRLLGKKTMENELLKEA
VEYGRQKKVDSARALVAGGWRISLVSRCLRVSRAQLHVILRRTDDWKDGRRSRGTDDTDALARIHHVIGELPTYGYRRVWALLRRQTELDGAAVINAKRV
YRIMRLNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAASTGGFDSETVQDVMLGAVERRFGSGLPASP
VEWLTDNGSCYRAYETRQFARIVGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAVKNLAEAFEHYNEWHPHSALGYRSPREYLRRRTSNG
LSDNRCLEI
VEYGRQKKVDSARALVAGGWRISLVSRCLRVSRAQLHVILRRTDDWKDGRRSRGTDDTDALARIHHVIGELPTYGYRRVWALLRRQTELDGAAVINAKRV
YRIMRLNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAASTGGFDSETVQDVMLGAVERRFGSGLPASP
VEWLTDNGSCYRAYETRQFARIVGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAVKNLAEAFEHYNEWHPHSALGYRSPREYLRRRTSNG
LSDNRCLEI
Blast result :
Comments
ISKmi2 is 95% aa similar to IS2.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Shuangshuang Li (2021) Direct submission.