ISKpn74

  • Family IS5
  • Group IS903
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
Y Klebsiella pneumoniae
Klebsiella pneumoniae
Klebsiella pneumoniae PBIO2030
Klebsiella pneumoniae PBIO2031
DNA section
IS Length : 1056 bp

Ends


IR Length : 17

IRL : GGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACG
IRR : GGCTTTGTTGAATAAATAAGATTTCGTGCGAACGACCCTGTAGCTGGCTG

Insertion site


Left flankDirect repeatRight flankDR Length
GACGTCGGTATTAAAGATGGCCTGATTGT9
CCAACAGAGAGACTGAACCCTACGGGAAA9
ATCACCGTATACGCTCGATATCAAATCCG9
GACAAGATATGCAAGTACCTGTAAACAGC9
CATTGATGTTCATCTGATGGTGAATCATC9
TGCAGTGCAGGGAAAATACATAAATAGTG9
TCGCTTACAGCCTTCGTGATGATAAAAAC9
CAGAGGACGTATACAGCTCAGAAAGATCCTGAAAGTTG0
CATAGTTCAA AGGAACGGCTATTTTTGG CTTCAATTTC0
AAGGCTTCAT TATTTTTGGAGGAACGGC ATGTTGCTCC0
AAAACCATAGCAATTGCGCCGTAAAAATATAAAACGGA0
CTACAAACCCCTTTTACTTATTTTCGCG ATAAGCAAAT0
ATAATTGTGCATGTCAAAGGAAAGAACTTTAAAAGCAA0
CTCTATGACG ATTTTCGCGGGCCGTGGCCGACGCCAGT0

DNA sequence

GGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACGCATCCTCCCGACAACTCAGACCATTCCGTGGCAAAGCAAAAGTTCAGAAT
CACCAACTGGTCCACTTACAACAAAGCTCTCATCAACCGTGGCTCCCTCACTTTCTGGCTGGATGATGAGGCGATTCAGGCCTGGTATGAGTCGGCAACG
CCTTCATCACGAGGAAGGCCCCAGCGCTATTCTGATCTCGCCATCACCACCGTTCTGGTGATTAAACGCGTATTCCGGCTGACCCTGCGGGCTGCGCAGG
GTTTTATTGATTCCATTTTTGCCCTGATGAACGTTCCGTTGCGCTGCCCGGATTACACCAGTGTCAGTAAGCGGGCAAAGTCGGTTAATGTCAGTTTCAA
AACGTCCACCCGGGGTGAAATCGCACACCTGGTGATTGATTCCACCGGGCTGAAGGTCTTTGGTGAAGGCGAATGGAAAGTCAGAAAGCACGGCAAAGAG
CGCCGTCGTATCTGGCGAAAGTTGCATCTTGCTGTTGACAGCAACACACATGAAGTTGTCTGTGCAGACCTGTCGCTGAATAACGTCACGGACTCAGAAG
CCTTCCCGGGCCTTATCCGGCAGACTCACAGAAAAATCAGGGCAGCCGCGGCAGACGGGGCTTACGATACACGGCTTTGTCACGATGAAATGCGCCGCAA
AAAAATCAGCGCGCTTATTCCTCCCCGAAAAGGAGCAGGTTACTGGCCCGGTGAGTACGCAGACCGCAACCGTGCCGTTGCTAATCAGCGGCTGAGCGGA
AGCAATGCACGGTGGAAATGGACAACGGAATATAACCGTCGCTCGATAGCGGAAACGGCAATGTACAGAATGAAGCAGTTGTTGGGAGATTCACTGACGC
TGCGTGACTACGATGGTCAGGTAGCGGAAGCTATGGCCATGGTGCGTGCGTTGAACAGGATGACAAAGGCTGGGATGCCAGAAAGCGTGCGTATTGCCTG
AAAATCCAGCCAGCTACAGGGTCGTTCGCACGAAATCTTATTTATTCAACAAAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
924 bp307 aa781001+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAKQKFRITNWSTYNKALINRGSLTFWLDDEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVPLRCPDYTSVSKRA
KSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRIWRKLHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRL
CHDEMRRKKISALIPPRKGAGYWPGEYADRNRAVANQRLSGSNARWKWTTEYNRRSIAETAMYRMKQLLGDSLTLRDYDGQVAEAMAMVRALNRMTKAGM
PESVRIA

 

Blast result :
Comments
ISKpn74 is 98% aa similar to IS903B.
There are 14 copies of ISKpn74 in the chromosome of K. pneumoniae PBIO2030. Two adjacent insertion sequences caused an inversion, backed up by the shuffling of direct repeats. The direct repeat in the left flank of the first insertion sequence (AGGAACGGC) is identical to the direct repeat in the right flank of the second insertion sequence and vice versa (TATTTTTGG). Additionally, K. pneumoniae PBIO2030 harbors a chromosomally inserted virulence plasmid which is framed by ISKpn74. The direct repeat in the right flank of one insertion sequence is identical to the direct repeat in the left flank of another insertion sequence (ATTTTCGCG). This is explainable by a co-integrate formation involving both elements. Please observe that the other two flanks share no identical direct repeat, possibly because of a deletion event involving yet another insertion sequence. Two IS elements which are located in the inserted plasmid share the same SNV (single-nucleotide variant; G->T, Lys3Asn) at position 86.
References
1] Stefan E. Heiden (2021) Direct submission.
2] Eger,E., Heiden,S.E., Becker,K., Rau,A., Geisenhainer,K., Idelevich,E.A., Schaufler,K. (2021) Plasmid. Int. J. Mol. Sci. 22(17):9196. https://doi.org/10.3390/ijms22179196