ISAba63
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP073061 | ND | Acinetobacter baumannii | Acinetobacter baumannii Acinetobacter baumannii plasmid pDETAB13 Acinetobacter baumannii plasmid pCAM180A Acinetobacter pittii HUMV-6483 plasmid p11 |
DNA section
IS Length : 1309 bp
Ends
IR Length : 25/27
IRL : TGAACCGTACCGGGTTTGTCGGAGAGTCAATATTCTGAGAGACTATCCCG
IRR : TGAAACGTACCGGGTTTGTCGGAGACTTTTTTATTTAAGTTAGGCCACCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTCAAAGTAG | ATCAGCCTTC | 0 |
DNA sequence
TGAACCGTACCGGGTTTGTCGGAGAGTCAATATTCTGAGAGACTATCCCGATGACAAAATTAAAATATACCCCTGAAATCAGAGAAAGAGCGGTTCAATT
ATTGATTGAATCTGAAAAAGATTATCCATCGAATTGGGCTGCGATCACCGCTATTGCTCCCAAGATAGGTTGTAGTCCTGAAACACTACGTGTTTGGTAT
CAAAAATATTTAGATAAACAAAATCCAGTTAAAGTACAGCAGCTTTCAGACCAAGAACGTATCAAACAACTCGAACGCGAAAATAAAGAACTGCAACGCG
CCAATGAGATTCTACGTAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTTATCCATAATAATAAACAGCTG
TACGGAGTCGAGGCGATTTGTAGAATTTTACCGATCGCACCTTCAACCTATTACCGGACTTTAGATCTCACAGTAAATCCAGAACATCGAGCGAAACGAG
ATTTACATGACTTGCATCATGCTGAGGAGATCAAGCGAATTTGGAAAGAAAGTTCAGGTCGATACGGTGTGCGTAAGGTCTGGCAACAACTGAAACGTGA
AGGCTATGTGATTGCACGTTGTACAGTTGCTCGATTGATGCAGAAGCTAGGTATACAAGGTGTTTGGCGTGGTAAGAATAAACAAACTACCCGTAGCCGA
GATGACCAAAAACGGGCAGATGATTTAGTGAAACGTAATTTTAGTGCTGATCATCCAAACCAACTATGGGTCGGTGACTTTACGTATATTCAAACTCATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTTTCACGAGCAATTGTTGGATGGAAAGTATCTACACGGATGAATACAGATATGGTGCTCGATGC
ATTGGAGCAAGCATTGCATGATCGAGGCATGCCAAAGAATGTGATTCATCATTCCGACAGAGGTGTGCAATATCTTTCCATTCGCTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACGACAGGCGATTCATACGATAATGCTTTGGCTGAAACGGTGAATGGCTTATACAAAACATAGGTGATTG
AATATCTAAAAGCAGTTTGGCAAGGTTTAGCAGATGTACAACTTGCGACACTAAACTGGGTAGATTGGTTCAATAAAAAGCGTGTACACAGTGCACTGGG
TTATGTATCGCCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCCTAACTTAAATAAAAAAGTCTCCGACAAACCCGG
TACGTTTCA
ATTGATTGAATCTGAAAAAGATTATCCATCGAATTGGGCTGCGATCACCGCTATTGCTCCCAAGATAGGTTGTAGTCCTGAAACACTACGTGTTTGGTAT
CAAAAATATTTAGATAAACAAAATCCAGTTAAAGTACAGCAGCTTTCAGACCAAGAACGTATCAAACAACTCGAACGCGAAAATAAAGAACTGCAACGCG
CCAATGAGATTCTACGTAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTTATCCATAATAATAAACAGCTG
TACGGAGTCGAGGCGATTTGTAGAATTTTACCGATCGCACCTTCAACCTATTACCGGACTTTAGATCTCACAGTAAATCCAGAACATCGAGCGAAACGAG
ATTTACATGACTTGCATCATGCTGAGGAGATCAAGCGAATTTGGAAAGAAAGTTCAGGTCGATACGGTGTGCGTAAGGTCTGGCAACAACTGAAACGTGA
AGGCTATGTGATTGCACGTTGTACAGTTGCTCGATTGATGCAGAAGCTAGGTATACAAGGTGTTTGGCGTGGTAAGAATAAACAAACTACCCGTAGCCGA
GATGACCAAAAACGGGCAGATGATTTAGTGAAACGTAATTTTAGTGCTGATCATCCAAACCAACTATGGGTCGGTGACTTTACGTATATTCAAACTCATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTTTCACGAGCAATTGTTGGATGGAAAGTATCTACACGGATGAATACAGATATGGTGCTCGATGC
ATTGGAGCAAGCATTGCATGATCGAGGCATGCCAAAGAATGTGATTCATCATTCCGACAGAGGTGTGCAATATCTTTCCATTCGCTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACGACAGGCGATTCATACGATAATGCTTTGGCTGAAACGGTGAATGGCTTATACAAAACATAGGTGATTG
AATATCTAAAAGCAGTTTGGCAAGGTTTAGCAGATGTACAACTTGCGACACTAAACTGGGTAGATTGGTTCAATAAAAAGCGTGTACACAGTGCACTGGG
TTATGTATCGCCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCCTAACTTAAATAAAAAAGTCTCCGACAAACCCGG
TACGTTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 105 aa | 51 | 368 | + | No |
Description : First part of the transposase
ORF sequence :
MTKLKYTPEIRERAVQLLIESEKDYPSNWAAITAIAPKIGCSPETLRVWYQKYLDKQNPVKVQQLSDQERIKQLERENKELQRANEILRKAAAFFAQAEL
DRPHK
DRPHK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 774 bp | 257 aa | 320 | 1093 | + | No |
Description : Second part of the transposase
ORF sequence :
SSRFFRPGGARPPTQIMVDFIHNNKQLYGVEAICRILPIAPSTYYRTLDLTVNPEHRAKRDLHDLHHAEEIKRIWKESSGRYGVRKVWQQLKREGYVIAR
CTVARLMQKLGIQGVWRGKNKQTTRSRDDQKRADDLVKRNFSADHPNQLWVGDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALH
DRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKT
CTVARLMQKLGIQGVWRGKNKQTTRSRDDQKRADDLVKRNFSADHPNQLWVGDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALH
DRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1043 bp | 347 aa | 51 | 1093 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKLKYTPEIRERAVQLLIESEKDYPSNWAAITAIAPKIGCSPETLRVWYQKYLDKQNPVKVQQLSDQERIKQLERENKELQRANEILRKAAAFFRPGGA
RPPTQIMVDFIHNNKQLYGVEAICRILPIAPSTYYRTLDLTVNPEHRAKRDLHDLHHAEEIKRIWKESSGRYGVRKVWQQLKREGYVIARCTVARLMQKL
GIQGVWRGKNKQTTRSRDDQKRADDLVKRNFSADHPNQLWVGDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKT
RPPTQIMVDFIHNNKQLYGVEAICRILPIAPSTYYRTLDLTVNPEHRAKRDLHDLHHAEEIKRIWKESSGRYGVRKVWQQLKREGYVIARCTVARLMQKL
GIQGVWRGKNKQTTRSRDDQKRADDLVKRNFSADHPNQLWVGDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKT
Blast result :
Comments
ISAba63 is 98% aa (transposase) similar to ISAlw4.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAba63 was found in plasmid pDETAB13 of an Acinetobacter baumannii isolated from a patient rectal swab in an intensive care unit in Hangzhou in 2019.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAba63 was found in plasmid pDETAB13 of an Acinetobacter baumannii isolated from a patient rectal swab in an intensive care unit in Hangzhou in 2019.
References
1] Robert Moran (2021) Direct submission.