ISAba64

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP073061 ND Acinetobacter baumannii
Acinetobacter baumannii
Acinetobacter baumannii plasmid pDETAB13
Acinetobacter baumannii plasmid pCAM180A
Acinetobacter pittii HUMV-6483 plasmid p11
DNA section
IS Length : 1318 bp

Ends


IR Length : 19/28

IRL : GGGACTGTTCTAAATTTTGTGTAAGTACTTAATTTTCATTTATCCTACTG
IRR : GAGCCTGTAAATTATTTTGTGTAAGTTCCATTTTTTATAAATGATCTTTT

Insertion site


Left flankDirect repeatRight flankDR Length
CAGTTTCATT-GTTTCAGGCT0

DNA sequence

GGGACTGTTCTAAATTTTGTGTAAGTACTTAATTTTCATTTATCCTACTGAGGATAATAAAAAGAGGTACTTCACATGGATGAAGCTACAATCAAAAGTA
TGGCAGCTGAATTAGCTAAAGGTTTAAAAACTCCTGAAGATTTAAACCAAATGACAGCCATATTTAAAAAGTTCATGATCGAAACCGCTCTGAATACTGA
GCCTTCTGAACATTTAGGCTATGAAAAGCATCAACCTAAGAAAGGATCTAATGCACGTAATGGTTTTAGCTCCAAAACAGTTCTAACACAAGATGGGCAG
CTTGCTTTAGATATCCCTCGTGATCGTGACGGCTCTTTTGAACCACAGATTATTAAGAAGCATCAAACGCGCATTACTAGCATGGATGATCAAATTCTTT
CGCTATATGCGAAAGGAATGACAAATAGAGAAATTGTTGCTTTTTTTAAAGAAATGTACGATGCCGATGTATCTGCCTCCCTTATTAGTAAGGTAACGGA
TGCTGTCATTGAACAAGTGACAGAATGGCAAAATAGAACACTCGATAGTCTTTATCCTGTTGTCTATTTAGATTGCATTGTTGTTAAGGTTCGTCAGCAT
TCAACAGTAATCAATAAATCAGTTTATTTAGCATTAGGCATTAACTTGGATGGCCAAAAAGAACTTCTTGGCAGGTGGCTTGCTCAAACAGAAGGTTCCA
AATTCTGGCTGTCAGTCATGACAGAGCTTAAAAATCGAGGAGTGCAAGATATTCTTGTTGCCTGTGTAGACGGGCTAAAAGGCTTTCCTGATGCGATAGC
CTCTGTCTACCCTCATACTGACATCCAGCTGTGTATCGTGCATGTTGTACGCAATAGCCTGAGATTTGTGAGCTGGAAGGACTACAAAGCTGTAACAGCA
GGATTGAAGGCGATCTATCAGGCAAGTACAGAAGAAAATGCTTTAAATTCCCTAGGTATCTTCTGCGATCAATGGAATCATCAGTATCCAAAAATTGGAA
AATCCTGGCGGGCAAATTGGGAAAATATCCGAACGATCTTTAGCTATCCTGCTGAAATACGTCATGCGATTTATACAACAAATGCAATTGAATCATTGAA
TAGCGTGATTCGCCATTCAACTAAAAAGAGAAAGATCTTCTCTTCTGATGATTCAGTGAAAAAGGTCATTTATTTAGCAACTTCAAATGCTGCTAAAAAA
TGGACAATGCCAATTCAAAATTGGCGTTTAGCGATGAATTGGTTTACGATTCAGTTCGATGATCGATTAAAAGATCATTTATAAAAAATGGAACTTACAC
AAAATAATTTACAGGCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1209 bp402 aa761284+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDEATIKSMAAELAKGLKTPEDLNQMTAIFKKFMIETALNTEPSEHLGYEKHQPKKGSNARNGFSSKTVLTQDGQLALDIPRDRDGSFEPQIIKKHQTRI
TSMDDQILSLYAKGMTNREIVAFFKEMYDADVSASLISKVTDAVIEQVTEWQNRTLDSLYPVVYLDCIVVKVRQHSTVINKSVYLALGINLDGQKELLGR
WLAQTEGSKFWLSVMTELKNRGVQDILVACVDGLKGFPDAIASVYPHTDIQLCIVHVVRNSLRFVSWKDYKAVTAGLKAIYQASTEENALNSLGIFCDQW
NHQYPKIGKSWRANWENIRTIFSYPAEIRHAIYTTNAIESLNSVIRHSTKKRKIFSSDDSVKKVIYLATSNAAKKWTMPIQNWRLAMNWFTIQFDDRLKD
HL

 

Blast result :
Comments
ISAba64 is 98% aa similar to ISAba26.
ISAba64 was found in plasmid pDETAB13 of an A. baumannii isolated from a patient rectal swab in an intensive care unit in Hangzhou in 2019.
References
1] Robert Moran (2021) Direct submission.