ISAso1
- Family IS1202
- Group ISAba32
Isoform ISAba54Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| JX101647 | ND | Acinetobacter soli | Acinetobacter soli Acinetobacter soli plasmid pM131-2 Acinetobacter pittii plasmid pC54_001 A. baumannii chromosome |
DNA section
IS Length : 1481 bp
Ends
IR Length : 20/26
IRL : TGTTAGAAACCATTTAAAGTGTCTGTATTCTCACCAATAAAAATGTCTGG
IRR : TGTTATTCACCAATTAAAATGTCTATGCGATAAACCATTTAAAATGTCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTATACGTAAT | GCTTG | ATATTTCTCTTT | 5 |
DNA sequence
TGTTAGAAACCATTTAAAGTGTCTGTATTCTCACCAATAAAAATGTCTGGTCTATGATGCGCATCAACATCATGGACTGGATATGAATCGAGATGCTGAT
CACTATGTCTGACAAGGAAATACAACGTATTGCAGTGCTGCAAGATGTTCGAGATCGACGTATTACACAAGTCCGTGCTGCTGAAATTCTGAATCTTTCA
ACCCGTCAAATTACCCGGTTATTGCACAAGCTCAAGCAAGACGGTATTTCAGGCCTGACACATGCCAGTCGTGGTCAACCGGGTCATCGTCGCCATGATG
AAGCCGTAAAGTCTGAATGCCTTTCCCTTATTTCTGAACATCTGCTGGGCTTTGGCCCAACCTTGGCGCATGAGAAGCTCAGCAGCATGTTTGACCTGAA
TATTCCTGTGGAAACGGTTCGTCGCTGGATGACTGCCAATGAACTCTGGATTCCGCGATCCAAGCGTCTGAAACGGCCATATCAGCCTCGATATAAGCGG
GATTGCTTCGGTGAACTGATCCAAATTGATGGCTCATATCATGACTGGTTTGAAGGTCGAGCTGCAAAATGCTGCTTGCTGGTTTATATCGATGATGCCA
CTGGAAAGCTGTTGCATCTGCGCTTTTGTGAGGCTGAAACCACCTTTGACTATATGCTTTCCACCCGAGCCTACATTGAACAATACGGCAAGCCGCTGGC
CTTTTATAGTGACAAACATTCGGTATTCAGAGTGAACCAGAAATCCAGTCAGGACAGTCAGATCACACAATTTGGCAGGATTCTGAATGAGTTAAACATT
GATATTATCTTTGCCAACTCCCCACAGGCCAAAGGTCGTGTGGAACGTGCTAACAGGACACTGCAGGATCGTCTGATCAAGGAGATGCGCCTGGAAGGCA
TCTGCTCGATTGCCGAAGCCAATGCATGGCTGCCCTGCTTTATTGAGCATTTCAATCAGAAGTTCGCCAAGTGTGCGAGAAATTCCAAGAACTTGCATCG
CCCTTTAACCGAATCTGATCTTGAACTGGAGGATATTTTCACCTGGCAGGAACCACGTAAGGTCACTAAGAATCTGACCATCACCTATGACAAATGTATT
TATATGCTTGAGCCAACAGTACTGAATCATAAGCTGGTTGGGCAATACATTTCATTTCTGGAGTATCCAGATGGCACTGTGGCCATCATGCATGAAGGAC
GGAAGATCAACTATAGCGTCTTCAATAAACTGGCTGGACTGCAGCAAAATGAAGTGGTTGAGAATAAACGGTTAGGCGCAGTCCTAGCTCATATTCAACA
ACAACACGAAGAGTTGGAAAAACAGAATAAACGTTCCCGTCTCAAGAAAAGTATGCCGAGCCGTAAAGCTCAGAAAGCTGTTATTGAACAAAGAAATTTA
AATCCTGTGCTTGACTCTTATGGTTAAAAACAGGACATTTTAAATGGTTTATCGCATAGACATTTTAATTGGTGAATAACA
CACTATGTCTGACAAGGAAATACAACGTATTGCAGTGCTGCAAGATGTTCGAGATCGACGTATTACACAAGTCCGTGCTGCTGAAATTCTGAATCTTTCA
ACCCGTCAAATTACCCGGTTATTGCACAAGCTCAAGCAAGACGGTATTTCAGGCCTGACACATGCCAGTCGTGGTCAACCGGGTCATCGTCGCCATGATG
AAGCCGTAAAGTCTGAATGCCTTTCCCTTATTTCTGAACATCTGCTGGGCTTTGGCCCAACCTTGGCGCATGAGAAGCTCAGCAGCATGTTTGACCTGAA
TATTCCTGTGGAAACGGTTCGTCGCTGGATGACTGCCAATGAACTCTGGATTCCGCGATCCAAGCGTCTGAAACGGCCATATCAGCCTCGATATAAGCGG
GATTGCTTCGGTGAACTGATCCAAATTGATGGCTCATATCATGACTGGTTTGAAGGTCGAGCTGCAAAATGCTGCTTGCTGGTTTATATCGATGATGCCA
CTGGAAAGCTGTTGCATCTGCGCTTTTGTGAGGCTGAAACCACCTTTGACTATATGCTTTCCACCCGAGCCTACATTGAACAATACGGCAAGCCGCTGGC
CTTTTATAGTGACAAACATTCGGTATTCAGAGTGAACCAGAAATCCAGTCAGGACAGTCAGATCACACAATTTGGCAGGATTCTGAATGAGTTAAACATT
GATATTATCTTTGCCAACTCCCCACAGGCCAAAGGTCGTGTGGAACGTGCTAACAGGACACTGCAGGATCGTCTGATCAAGGAGATGCGCCTGGAAGGCA
TCTGCTCGATTGCCGAAGCCAATGCATGGCTGCCCTGCTTTATTGAGCATTTCAATCAGAAGTTCGCCAAGTGTGCGAGAAATTCCAAGAACTTGCATCG
CCCTTTAACCGAATCTGATCTTGAACTGGAGGATATTTTCACCTGGCAGGAACCACGTAAGGTCACTAAGAATCTGACCATCACCTATGACAAATGTATT
TATATGCTTGAGCCAACAGTACTGAATCATAAGCTGGTTGGGCAATACATTTCATTTCTGGAGTATCCAGATGGCACTGTGGCCATCATGCATGAAGGAC
GGAAGATCAACTATAGCGTCTTCAATAAACTGGCTGGACTGCAGCAAAATGAAGTGGTTGAGAATAAACGGTTAGGCGCAGTCCTAGCTCATATTCAACA
ACAACACGAAGAGTTGGAAAAACAGAATAAACGTTCCCGTCTCAAGAAAAGTATGCCGAGCCGTAAAGCTCAGAAAGCTGTTATTGAACAAAGAAATTTA
AATCCTGTGCTTGACTCTTATGGTTAAAAACAGGACATTTTAAATGGTTTATCGCATAGACATTTTAATTGGTGAATAACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1323 bp | 444 aa | 105 | 1427 | + | No |
Chemistry : DDE
ORF sequence :
MSDKEIQRIAVLQDVRDRRITQVRAAEILNLSTRQITRLLHKLKQDGISGLTHASRGQPGHRRHDEAVKSECLSLISEHLLGFGPTLAHEKLSSMFDLNI
PVETVRRWMTANELWIPRSKRLKRPYQPRYKRDCFGELIQIDGSYHDWFEGRAAKCCLLVYIDDATGKLLHLRFCEAETTFDYMLSTRAYIEQYGKPLAF
YSDKHSVFRVNQKSSQDSQITQFGRILNELNIDIIFANSPQAKGRVERANRTLQDRLIKEMRLEGICSIAEANAWLPCFIEHFNQKFAKCARNSKNLHRP
LTESDLELEDIFTWQEPRKVTKNLTITYDKCIYMLEPTVLNHKLVGQYISFLEYPDGTVAIMHEGRKINYSVFNKLAGLQQNEVVENKRLGAVLAHIQQQ
HEELEKQNKRSRLKKSMPSRKAQKAVIEQRNLNPVLDSYG
PVETVRRWMTANELWIPRSKRLKRPYQPRYKRDCFGELIQIDGSYHDWFEGRAAKCCLLVYIDDATGKLLHLRFCEAETTFDYMLSTRAYIEQYGKPLAF
YSDKHSVFRVNQKSSQDSQITQFGRILNELNIDIIFANSPQAKGRVERANRTLQDRLIKEMRLEGICSIAEANAWLPCFIEHFNQKFAKCARNSKNLHRP
LTESDLELEDIFTWQEPRKVTKNLTITYDKCIYMLEPTVLNHKLVGQYISFLEYPDGTVAIMHEGRKINYSVFNKLAGLQQNEVVENKRLGAVLAHIQQQ
HEELEKQNKRSRLKKSMPSRKAQKAVIEQRNLNPVLDSYG
Blast result :
Comments
ISAso1 is 100% aa similar to ISAba54.
ISAso1 was found in pM131-2, the GR13 plasmid of an Acinetobacter soli isolated from human sputum in Taiwan in 2010. ISAso1 is inserted in the sul2-containing dif module of pM131-2.
ISAso1 was found in pM131-2, the GR13 plasmid of an Acinetobacter soli isolated from human sputum in Taiwan in 2010. ISAso1 is inserted in the sul2-containing dif module of pM131-2.
References
1] Robert Moran (2021) Direct submission.
2] Huang, T.-W., Lauderdale, T.-L., Liao, T.-L., Hsu, M.-C., Chang, F.-Y., Chang, S.-C., Khong, W.X., Ng, O.T., Chen, Y.-T., Kuo, S.-C., Chen, T.-L., Mu, J.-J., Tsai, S.-F., (2015) Effective transfer of a 47 kb NDM-1-positive plasmid among Acinetobacter species. J. Antimicrob. Chemother. 70, 2734–2738.
2] Huang, T.-W., Lauderdale, T.-L., Liao, T.-L., Hsu, M.-C., Chang, F.-Y., Chang, S.-C., Khong, W.X., Ng, O.T., Chen, Y.-T., Kuo, S.-C., Chen, T.-L., Mu, J.-J., Tsai, S.-F., (2015) Effective transfer of a 47 kb NDM-1-positive plasmid among Acinetobacter species. J. Antimicrob. Chemother. 70, 2734–2738.