ISAso2
- Family IS1202
- Group ISAba32
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| JX101647 | ND | Acinetobacter soli | Acinetobacter soli Acinetobacter soli plasmid pM131-2 |
DNA section
IS Length : 1482 bp
Ends
IR Length : 20/24
IRL : TGTAAGCCCCAAGTAGAAATGTCCGGTTTCTCCAAAGTAAAAATGTCCGC
IRR : TGTTATTCCTAAGTAGAAATGTCCTACCTAATAACCAAATAGAAATGTCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATACGAAAC | GAGAG | TGTTAGAACC | 5 |
DNA sequence
TGTAAGCCCCAAGTAGAAATGTCCGGTTTCTCCAAAGTAAAAATGTCCGCTTTTAGAATATGCACTTTTGCAATTTCGTTAGCGGACGGTTTGATATGTT
GGTGTCTATGTCGGATAAAGAACTTAAACGATTGTCGGTCTTGCAGGAAATCTGCGATCAACGCATAACCCAGTCCCAAGCTGCTCAGCTACTTCATATT
TCAGAACGTCAGATCAGACGCTTACTGCAGAAATACAAAGCTCAAGGTCCCGCTGCATTAGCCCATGCCGGTCGTGGCCAAATCAGCAATTCCAAACTTC
CTGAAGAACTCAGACTCAAGTGCCTCAATATTGTTTCTGACCAACTCCATGGTTTCGGACCCACTTTAGCGCATGAAAAGCTCACCACCGTACATGGATT
CGATCTTTCAGTAGAAACCCTGCGTTCCTGGATGATTGTAGCTGACTTATGGATTCCTCGATCCAAGCGCCTGAAACGCCCGTATCAGCCTCGTTACAAC
CGGGATTGCTTTGGTGAACTGATCCAAATTGATGGCTCATATCATGACTGGTTTGAAGGACGCGCTGCTAAATGCTGTCTGCTGGTGTTTATCGATGATG
CTACAGGAAAATTGCAGCATTTACGCTTCTGTGAATCAGAATCAGCCTTTGATTATATGATTTCAACACGCTTGTATGTTGAGCAGCATGGTAAGCCGTT
GGCGTTTTACAGTGATAAACATTCAGTCTTCAGGGTGAATCAAAGCAGCAAGAAAGACACCAAGATTACCCAGTTTGGACGCGTACTCAGTACCCTCAAT
ATCGATATCATCTTCGCCAATTCACCACAAGCCAAAGGCCGTGTAGAACGGGCGAATAGAACGCTTCAGGACCGTCTGATTAAAGAAATGCGCCTGGAAG
GCATCTGTTCGATTGAGCAAGCGAATGCCTGGCTGCCCTGCTTTATCGAGCAATTTAATCGTAAATTTGCCAATATGGCCTTTAATCCCAAGGATCTACA
CCGGACTGTCACTGAAACAGCCGAAGAATTAGATGATGTTTTTACCTGGCGTGAACCCCGCAGAGTCACGAACAGCCTGACGATTACTTATGATAAATGC
GTATATTTACTGGAAAATACCGAAGAAAATAAAAGGTTGATCGGCAAGTATCTTGAGTTTCTGGAATACCCGGATGGCACGGTAGCCATTGAACATCAAG
GAAGAAAAATCAATTACAGCATCTTCGATAAATTAAGTCAGCTCAACCAGCGAGAGGTTGTTGAGAATAAACGTTTAGGTGCTGTTCTCAATCATATTCA
ACAACAGCACGAAGAATTAGAACAGCAAAACAAACGCAATCGTTCTCAATCTATGCCAAAAAGACGTGCACAGAAAACAGCAATTCAACAACGAAATCTG
AATCCTGTGCTTGACTTGGAAGTGTCTGTATAGGACATTTCTATTTGGTTATTAGGTAGGACATTTCTACTTAGGAATAACA
GGTGTCTATGTCGGATAAAGAACTTAAACGATTGTCGGTCTTGCAGGAAATCTGCGATCAACGCATAACCCAGTCCCAAGCTGCTCAGCTACTTCATATT
TCAGAACGTCAGATCAGACGCTTACTGCAGAAATACAAAGCTCAAGGTCCCGCTGCATTAGCCCATGCCGGTCGTGGCCAAATCAGCAATTCCAAACTTC
CTGAAGAACTCAGACTCAAGTGCCTCAATATTGTTTCTGACCAACTCCATGGTTTCGGACCCACTTTAGCGCATGAAAAGCTCACCACCGTACATGGATT
CGATCTTTCAGTAGAAACCCTGCGTTCCTGGATGATTGTAGCTGACTTATGGATTCCTCGATCCAAGCGCCTGAAACGCCCGTATCAGCCTCGTTACAAC
CGGGATTGCTTTGGTGAACTGATCCAAATTGATGGCTCATATCATGACTGGTTTGAAGGACGCGCTGCTAAATGCTGTCTGCTGGTGTTTATCGATGATG
CTACAGGAAAATTGCAGCATTTACGCTTCTGTGAATCAGAATCAGCCTTTGATTATATGATTTCAACACGCTTGTATGTTGAGCAGCATGGTAAGCCGTT
GGCGTTTTACAGTGATAAACATTCAGTCTTCAGGGTGAATCAAAGCAGCAAGAAAGACACCAAGATTACCCAGTTTGGACGCGTACTCAGTACCCTCAAT
ATCGATATCATCTTCGCCAATTCACCACAAGCCAAAGGCCGTGTAGAACGGGCGAATAGAACGCTTCAGGACCGTCTGATTAAAGAAATGCGCCTGGAAG
GCATCTGTTCGATTGAGCAAGCGAATGCCTGGCTGCCCTGCTTTATCGAGCAATTTAATCGTAAATTTGCCAATATGGCCTTTAATCCCAAGGATCTACA
CCGGACTGTCACTGAAACAGCCGAAGAATTAGATGATGTTTTTACCTGGCGTGAACCCCGCAGAGTCACGAACAGCCTGACGATTACTTATGATAAATGC
GTATATTTACTGGAAAATACCGAAGAAAATAAAAGGTTGATCGGCAAGTATCTTGAGTTTCTGGAATACCCGGATGGCACGGTAGCCATTGAACATCAAG
GAAGAAAAATCAATTACAGCATCTTCGATAAATTAAGTCAGCTCAACCAGCGAGAGGTTGTTGAGAATAAACGTTTAGGTGCTGTTCTCAATCATATTCA
ACAACAGCACGAAGAATTAGAACAGCAAAACAAACGCAATCGTTCTCAATCTATGCCAAAAAGACGTGCACAGAAAACAGCAATTCAACAACGAAATCTG
AATCCTGTGCTTGACTTGGAAGTGTCTGTATAGGACATTTCTATTTGGTTATTAGGTAGGACATTTCTACTTAGGAATAACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1374 bp | 457 aa | 60 | 1433 | + | No |
Chemistry : DDE
ORF sequence :
MHFCNFVSGRFDMLVSMSDKELKRLSVLQEICDQRITQSQAAQLLHISERQIRRLLQKYKAQGPAALAHAGRGQISNSKLPEELRLKCLNIVSDQLHGFG
PTLAHEKLTTVHGFDLSVETLRSWMIVADLWIPRSKRLKRPYQPRYNRDCFGELIQIDGSYHDWFEGRAAKCCLLVFIDDATGKLQHLRFCESESAFDYM
ISTRLYVEQHGKPLAFYSDKHSVFRVNQSSKKDTKITQFGRVLSTLNIDIIFANSPQAKGRVERANRTLQDRLIKEMRLEGICSIEQANAWLPCFIEQFN
RKFANMAFNPKDLHRTVTETAEELDDVFTWREPRRVTNSLTITYDKCVYLLENTEENKRLIGKYLEFLEYPDGTVAIEHQGRKINYSIFDKLSQLNQREV
VENKRLGAVLNHIQQQHEELEQQNKRNRSQSMPKRRAQKTAIQQRNLNPVLDLEVSV
PTLAHEKLTTVHGFDLSVETLRSWMIVADLWIPRSKRLKRPYQPRYNRDCFGELIQIDGSYHDWFEGRAAKCCLLVFIDDATGKLQHLRFCESESAFDYM
ISTRLYVEQHGKPLAFYSDKHSVFRVNQSSKKDTKITQFGRVLSTLNIDIIFANSPQAKGRVERANRTLQDRLIKEMRLEGICSIEQANAWLPCFIEQFN
RKFANMAFNPKDLHRTVTETAEELDDVFTWREPRRVTNSLTITYDKCVYLLENTEENKRLIGKYLEFLEYPDGTVAIEHQGRKINYSIFDKLSQLNQREV
VENKRLGAVLNHIQQQHEELEQQNKRNRSQSMPKRRAQKTAIQQRNLNPVLDLEVSV
Blast result :
Comments
ISAso2 is 98% aa similar to ISAjo2.
ISAso2 was found in pM131-2, the GR13 plasmid of an Acinetobacter soli isolated from human sputum in Taiwan in 2010. ISAso2 is inserted in a blaOXA-58-containing dif module.
ISAso2 was found in pM131-2, the GR13 plasmid of an Acinetobacter soli isolated from human sputum in Taiwan in 2010. ISAso2 is inserted in a blaOXA-58-containing dif module.
References
1] Robert Moran (2021) Direct submission.
2] Huang, T.-W., Lauderdale, T.-L., Liao, T.-L., Hsu, M.-C., Chang, F.-Y., Chang, S.-C., Khong, W.X., Ng, O.T., Chen, Y.-T., Kuo, S.-C., Chen, T.-L., Mu, J.-J., Tsai, S.-F., (2015) J. Antimicrob. Chemother. 70, 2734–2738.
2] Huang, T.-W., Lauderdale, T.-L., Liao, T.-L., Hsu, M.-C., Chang, F.-Y., Chang, S.-C., Khong, W.X., Ng, O.T., Chen, Y.-T., Kuo, S.-C., Chen, T.-L., Mu, J.-J., Tsai, S.-F., (2015) J. Antimicrob. Chemother. 70, 2734–2738.