ISApi1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP027251 | ND | Acinetobacter pittii | Acinetobacter pittii Acinetobacter pittii plasmid p1_100020 Acinetobacter nosocomialis Acinetobacter pittii plasmid p1_005046 |
DNA section
IS Length : 1237 bp
Ends
IR Length : 30/39
IRL : TGATCCTAGCCAAAATAATGGACAGTACTTCCCTCTAAAGATAACGTAAC
IRR : TGATCCTAGACAGTATCGTGGACAACAAATCCCTCTAAATTCTTAAAATA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCATTCCTTA | AGC | TTTCCTTGGA | 3 |
| GTCGAAGATT | AGC | TGATATGTAT | 3 |
| AGAACAACAG | GACCTTATAT | 0 | |
| AACCTGTCTC | ATCTTCTAAT | 0 | |
| AGTTCTCTAT | ATG | ATTTGTATTA | 3 |
| TGTAGCAGAT | GTG | GCTTCAATCG | 3 |
| AAGACCTTAA | GCC | CTTAAATGAG | 3 |
| GTCACATTTA | CAG | ATGAGTTTGC | 3 |
| TCGTTTAATA | CCT | GCATCAGATT | 3 |
DNA sequence
TGATCCTAGCCAAAATAATGGACAGTACTTCCCTCTAAAGATAACGTAACGTTAACTTTGGAGATCCAAGAAATGGCCAAACGTTTTAGTGCTGAATTTA
AACAGCAAGCAATTGATTATGCACTTTCAAACTCACACGAGCCTATAGCTGCAATCGCCCAGAAATTAGGTGTGGGTTATTCAACTTTAGACAAATGGAT
TCGTGAAACCAATCCAGTGGGTTCAAGCAAACGTCAACTTTCACCAGAACAACAGCGGATCTTGGAATTAGAGAAAGAAGTCAAACAGCTCAAGGAAGCC
AATGACATCTTAAAAAAAGCGCATGTGTACTTTCTAACAGATCATGCCAAGAAAAGTACACGGTAATTCAAGATCTAGATATGAATGAAGTCACGGTATC
TTCTGCCTGTAAATACCTAGGTGTCAGCACTTCAGGCTATTATGCCTGGCGAAAACGTCAGGCCAATACGGTAGAGAAATATAATGATTTAAAAGCCGTA
TATTGGCAGCATCATGCACGATTGGGTGCACCTTCATTGGTACATGACATGCATGATTTAGGTTACAGCATGAGTGAACGCACTGTTGGAAGAATGCTGA
AAAATCTGGGTTTACGCAGCAGGATTGCGCGTAAATACAAGCATACGACTGATTCAAACCATCGTTTGCCTACAGCACCCAATCTGTTAGAGCGTCAATT
TACAGTCATTCAGCCTAACAAGGTTTGGACAACGGATATTACCTATATCCGTACCAAGCAAGGTTGGCTGTATTTATGTGTGATGCTGGATCTATTTAGT
CGTCGTATCGTGGGGTGGCAAACCAGCCATCGAATAGACCGCCAGTTGGTGTGTGATACGTTTAACTATGCAATGGCTCGTCAGGGTTATCCAACTGGTG
TTATGGTTCATTCGGACCAAGGTTCACAGTACTGTAGTCGTGATTTTAGAGCGCTGTTACTGACAAATGATTGTACTCAGAGTATGTCTAGACGTGGAAA
CTGTTGGGATAATGCAGTGACTGAAAGCTTCTTTCATACATTGAAAGGTCATGTGGTCCATGGCAGTGTGTTTGCCACTCGAAAAGAAGCGAATGCTGTC
TTGTTTGACTATATTGAGATTTATTACAATCGGGTCAGAAGACATTCTGCAAATGGCTGGTTAAGCCCTGAAGCCTTTGAGAAGAAATATTTTAAGAATT
TAGAGGGATTTGTTGTCCACGATACTGTCTAGGATCA
AACAGCAAGCAATTGATTATGCACTTTCAAACTCACACGAGCCTATAGCTGCAATCGCCCAGAAATTAGGTGTGGGTTATTCAACTTTAGACAAATGGAT
TCGTGAAACCAATCCAGTGGGTTCAAGCAAACGTCAACTTTCACCAGAACAACAGCGGATCTTGGAATTAGAGAAAGAAGTCAAACAGCTCAAGGAAGCC
AATGACATCTTAAAAAAAGCGCATGTGTACTTTCTAACAGATCATGCCAAGAAAAGTACACGGTAATTCAAGATCTAGATATGAATGAAGTCACGGTATC
TTCTGCCTGTAAATACCTAGGTGTCAGCACTTCAGGCTATTATGCCTGGCGAAAACGTCAGGCCAATACGGTAGAGAAATATAATGATTTAAAAGCCGTA
TATTGGCAGCATCATGCACGATTGGGTGCACCTTCATTGGTACATGACATGCATGATTTAGGTTACAGCATGAGTGAACGCACTGTTGGAAGAATGCTGA
AAAATCTGGGTTTACGCAGCAGGATTGCGCGTAAATACAAGCATACGACTGATTCAAACCATCGTTTGCCTACAGCACCCAATCTGTTAGAGCGTCAATT
TACAGTCATTCAGCCTAACAAGGTTTGGACAACGGATATTACCTATATCCGTACCAAGCAAGGTTGGCTGTATTTATGTGTGATGCTGGATCTATTTAGT
CGTCGTATCGTGGGGTGGCAAACCAGCCATCGAATAGACCGCCAGTTGGTGTGTGATACGTTTAACTATGCAATGGCTCGTCAGGGTTATCCAACTGGTG
TTATGGTTCATTCGGACCAAGGTTCACAGTACTGTAGTCGTGATTTTAGAGCGCTGTTACTGACAAATGATTGTACTCAGAGTATGTCTAGACGTGGAAA
CTGTTGGGATAATGCAGTGACTGAAAGCTTCTTTCATACATTGAAAGGTCATGTGGTCCATGGCAGTGTGTTTGCCACTCGAAAAGAAGCGAATGCTGTC
TTGTTTGACTATATTGAGATTTATTACAATCGGGTCAGAAGACATTCTGCAAATGGCTGGTTAAGCCCTGAAGCCTTTGAGAAGAAATATTTTAAGAATT
TAGAGGGATTTGTTGTCCACGATACTGTCTAGGATCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 73 | 366 | + | No |
Description : First part of the transposase
ORF sequence :
MAKRFSAEFKQQAIDYALSNSHEPIAAIAQKLGVGYSTLDKWIRETNPVGSSKRQLSPEQQRILELEKEVKQLKEANDILKKAHVYFLTDHAKKSTR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 927 bp | 308 aa | 306 | 1232 | + | No |
Description : Second part of the transposase
ORF sequence :
HLKKSACVLSNRSCQEKYTVIQDLDMNEVTVSSACKYLGVSTSGYYAWRKRQANTVEKYNDLKAVYWQHHARLGAPSLVHDMHDLGYSMSERTVGRMLKN
LGLRSRIARKYKHTTDSNHRLPTAPNLLERQFTVIQPNKVWTTDITYIRTKQGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDTFNYAMARQGYPTGVM
VHSDQGSQYCSRDFRALLLTNDCTQSMSRRGNCWDNAVTESFFHTLKGHVVHGSVFATRKEANAVLFDYIEIYYNRVRRHSANGWLSPEAFEKKYFKNLE
GFVVHDTV
LGLRSRIARKYKHTTDSNHRLPTAPNLLERQFTVIQPNKVWTTDITYIRTKQGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDTFNYAMARQGYPTGVM
VHSDQGSQYCSRDFRALLLTNDCTQSMSRRGNCWDNAVTESFFHTLKGHVVHGSVFATRKEANAVLFDYIEIYYNRVRRHSANGWLSPEAFEKKYFKNLE
GFVVHDTV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1160 bp | 386 aa | 73 | 1232 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKRFSAEFKQQAIDYALSNSHEPIAAIAQKLGVGYSTLDKWIRETNPVGSSKRQLSPEQQRILELEKEVKQLKEANDILKKSACVLSNRSCQEKYTVIQ
DLDMNEVTVSSACKYLGVSTSGYYAWRKRQANTVEKYNDLKAVYWQHHARLGAPSLVHDMHDLGYSMSERTVGRMLKNLGLRSRIARKYKHTTDSNHRLP
TAPNLLERQFTVIQPNKVWTTDITYIRTKQGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDTFNYAMARQGYPTGVMVHSDQGSQYCSRDFRALLLTND
CTQSMSRRGNCWDNAVTESFFHTLKGHVVHGSVFATRKEANAVLFDYIEIYYNRVRRHSANGWLSPEAFEKKYFKNLEGFVVHDTV
DLDMNEVTVSSACKYLGVSTSGYYAWRKRQANTVEKYNDLKAVYWQHHARLGAPSLVHDMHDLGYSMSERTVGRMLKNLGLRSRIARKYKHTTDSNHRLP
TAPNLLERQFTVIQPNKVWTTDITYIRTKQGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDTFNYAMARQGYPTGVMVHSDQGSQYCSRDFRALLLTND
CTQSMSRRGNCWDNAVTESFFHTLKGHVVHGSVFATRKEANAVLFDYIEIYYNRVRRHSANGWLSPEAFEKKYFKNLEGFVVHDTV
Blast result :
Comments
ISApi1 was found in p1_100020, a GR13-type plasmid from Acinetobacter pittii WCHAP100020, which was isolated in Chengu, China in 2015. Two copies of ISApi1 are present in the WCHAP100020 chromosome. This insertion sequence is also present in other species of this genus, such as Acinetobacter nosocomialis.
Accession numbers : CP027254.3, CP027251.3, CP033561.1, CP028569.1, CP028574.2.
Accession numbers : CP027254.3, CP027251.3, CP033561.1, CP028569.1, CP028574.2.
References
1] Tomas Liveikis (2022) Direct submission.
2] Long,H., Ye,H., Feng,Y. and Wang,X. (2019) Direct GenBank submission.
2] Long,H., Ye,H., Feng,Y. and Wang,X. (2019) Direct GenBank submission.