ISApi2
- Family IS1202
- Group ISAba32
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP033536 | ND | Acinetobacter pittii | Acinetobacter pittii Acinetobacter pittii plasmid p2012N21-164-1 Acinetobacter baumannii plasmid pAb-C102_1 |
DNA section
IS Length : 1482 bp
Ends
IR Length : 19/24
IRL : TGTAAGCCCAAAATAGAAATGTCCGGTTTCTCCAAAGTAAAAATGTCCGC
IRR : TGTTATTCCCAAGTAGAAATGTCCTACCTAATAACCAAATAGAAATGTCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATGCGAATC | TTGAA | AGATTAAGCT | 5 |
| TATGCGAACA | GTTGT | ATAATTCAAT | 5 |
| TATACGAAAC | GAGAG | AGTGTTAGAA | 5 |
DNA sequence
TGTAAGCCCAAAATAGAAATGTCCGGTTTCTCCAAAGTAAAAATGTCCGCTTTTAGAATATGCGCTTTTGCAATTTCCTTAGCGGACGGTTTGATATGTT
GGTGTCTATGTCGGATAAAGAACTTAAACGATTGTCGGTCTTGCAAGAAATCTGTGATCAACGTATAACTCAATCCCAGGCTGCTCAGCTACTTCATATC
TCAGAACGTCACATCAGACGCTTACTGCAGAAATACAAAGCTCAAGGTCCCGCTGCATTAGCCCATGCCGGTCGTGGCCAAATCAGCAATTCTAAACTTC
CTGAAGAACTCAGACTTAAGTGTCTCAATATTGTTTCAGATCAACTTTATGGTTTCGGACCCACTTTAGCGCATGAAAAGCTCACCACCGTGCATGGATT
CGATCTTTCAGTAGAAACCCTGCGTTCCTGGATGATTGCAGCCGATTTATGGATCCCTCGCTGCAAGCGCCTGAAACGACCATATCAGCCTCGTTATAAC
CGAGATTGTTATGGTGAATTGATCCAAATTGATGGCTCACACCATGATTGGTTTGAAGGTCGAGCACCTAAATGCTGTCTGCTAGTATTCATCGATGATG
CCACAGGAAAATTGCAGCATTTACGCTTCTGTGCGTCAGAGTCAGCCTTTGACTATATGATTTCGACACGTTTATATATCGAACAGCATGGTAAGCCATT
GGCATTTTACAGTGACAAACATTCAGTTTTTAGGGTGAATCAAAGTAGCAAGAAAGACACCAAGATTACCCAGTTTGGACGTGTTCTGAGCACTCTCAAT
ATCGATATCATTTTCGCTAACTCCCCTCAAGCCAAAGGTCGTGTAGAACGAGCGAATAGAACACTTCAGGATCGTCTGATTAAGGAGATGCGTCTAGAAG
GCATCAGTTCAATTGCAGATGCCAATAAGTGGCTGCCGTGCTTTATTGAGCAATTTAATCGTAAATTCGCCAAGATGGCTTTTAATTCCAAGGATCTACA
TCGGCCTATCGCTGAAACAGCCGAAGAATTAGATGATGTTTTTACCTGGCGTGAACCCCGCAGGGTCACGAACAGCTTGACGATTACTTATGATAAATGC
GTATATATCTTGGAAAACAGCGAAGAAAATCAAAGATTGGGCGGCAAGTATCTTGAGTTCCTGGAATATCCGGATGGTACTGTAGCCATTGAATATCAGG
GAAGAAAAATCAATTACAGCATCTTTGATAAATTAAGTCAGCTCAACCAGCGAGAGATCGTTGAGAATAAACGTTTGGGTTCTGTGCTGGCACATATTCA
ACAACAGCATGAACAACTGGAACACCAAAACAAACGCAATCGTTCTCAAAAGATGCCACGTAGACAAGCACAGAAAACAGCGATTGAACAACGAAATCTA
AATCCTGTGCTTGACTTGGAAATGTCCATATAGGACATTTCTATTTGGTTATTAGGTAGGACATTTCTACTTGGGAATAACA
GGTGTCTATGTCGGATAAAGAACTTAAACGATTGTCGGTCTTGCAAGAAATCTGTGATCAACGTATAACTCAATCCCAGGCTGCTCAGCTACTTCATATC
TCAGAACGTCACATCAGACGCTTACTGCAGAAATACAAAGCTCAAGGTCCCGCTGCATTAGCCCATGCCGGTCGTGGCCAAATCAGCAATTCTAAACTTC
CTGAAGAACTCAGACTTAAGTGTCTCAATATTGTTTCAGATCAACTTTATGGTTTCGGACCCACTTTAGCGCATGAAAAGCTCACCACCGTGCATGGATT
CGATCTTTCAGTAGAAACCCTGCGTTCCTGGATGATTGCAGCCGATTTATGGATCCCTCGCTGCAAGCGCCTGAAACGACCATATCAGCCTCGTTATAAC
CGAGATTGTTATGGTGAATTGATCCAAATTGATGGCTCACACCATGATTGGTTTGAAGGTCGAGCACCTAAATGCTGTCTGCTAGTATTCATCGATGATG
CCACAGGAAAATTGCAGCATTTACGCTTCTGTGCGTCAGAGTCAGCCTTTGACTATATGATTTCGACACGTTTATATATCGAACAGCATGGTAAGCCATT
GGCATTTTACAGTGACAAACATTCAGTTTTTAGGGTGAATCAAAGTAGCAAGAAAGACACCAAGATTACCCAGTTTGGACGTGTTCTGAGCACTCTCAAT
ATCGATATCATTTTCGCTAACTCCCCTCAAGCCAAAGGTCGTGTAGAACGAGCGAATAGAACACTTCAGGATCGTCTGATTAAGGAGATGCGTCTAGAAG
GCATCAGTTCAATTGCAGATGCCAATAAGTGGCTGCCGTGCTTTATTGAGCAATTTAATCGTAAATTCGCCAAGATGGCTTTTAATTCCAAGGATCTACA
TCGGCCTATCGCTGAAACAGCCGAAGAATTAGATGATGTTTTTACCTGGCGTGAACCCCGCAGGGTCACGAACAGCTTGACGATTACTTATGATAAATGC
GTATATATCTTGGAAAACAGCGAAGAAAATCAAAGATTGGGCGGCAAGTATCTTGAGTTCCTGGAATATCCGGATGGTACTGTAGCCATTGAATATCAGG
GAAGAAAAATCAATTACAGCATCTTTGATAAATTAAGTCAGCTCAACCAGCGAGAGATCGTTGAGAATAAACGTTTGGGTTCTGTGCTGGCACATATTCA
ACAACAGCATGAACAACTGGAACACCAAAACAAACGCAATCGTTCTCAAAAGATGCCACGTAGACAAGCACAGAAAACAGCGATTGAACAACGAAATCTA
AATCCTGTGCTTGACTTGGAAATGTCCATATAGGACATTTCTATTTGGTTATTAGGTAGGACATTTCTACTTGGGAATAACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1374 bp | 457 aa | 60 | 1433 | + | No |
Chemistry : DDE
ORF sequence :
MRFCNFLSGRFDMLVSMSDKELKRLSVLQEICDQRITQSQAAQLLHISERHIRRLLQKYKAQGPAALAHAGRGQISNSKLPEELRLKCLNIVSDQLYGFG
PTLAHEKLTTVHGFDLSVETLRSWMIAADLWIPRCKRLKRPYQPRYNRDCYGELIQIDGSHHDWFEGRAPKCCLLVFIDDATGKLQHLRFCASESAFDYM
ISTRLYIEQHGKPLAFYSDKHSVFRVNQSSKKDTKITQFGRVLSTLNIDIIFANSPQAKGRVERANRTLQDRLIKEMRLEGISSIADANKWLPCFIEQFN
RKFAKMAFNSKDLHRPIAETAEELDDVFTWREPRRVTNSLTITYDKCVYILENSEENQRLGGKYLEFLEYPDGTVAIEYQGRKINYSIFDKLSQLNQREI
VENKRLGSVLAHIQQQHEQLEHQNKRNRSQKMPRRQAQKTAIEQRNLNPVLDLEMSI
PTLAHEKLTTVHGFDLSVETLRSWMIAADLWIPRCKRLKRPYQPRYNRDCYGELIQIDGSHHDWFEGRAPKCCLLVFIDDATGKLQHLRFCASESAFDYM
ISTRLYIEQHGKPLAFYSDKHSVFRVNQSSKKDTKITQFGRVLSTLNIDIIFANSPQAKGRVERANRTLQDRLIKEMRLEGISSIADANKWLPCFIEQFN
RKFAKMAFNSKDLHRPIAETAEELDDVFTWREPRRVTNSLTITYDKCVYILENSEENQRLGGKYLEFLEYPDGTVAIEYQGRKINYSIFDKLSQLNQREI
VENKRLGSVLAHIQQQHEQLEHQNKRNRSQKMPRRQAQKTAIEQRNLNPVLDLEMSI
Blast result :
Comments
ISApi2 is 96% aa similar to ISAso2.
ISApi2 was found in the GR13-type plasmid p2012N21-164-1 from an Acinetobacter pittii, where it is inserted immediately adjacent to a pdif site and flanked by a 5 bp target site duplication.
Additionnal Accession numbers : CP033536, CP033535, CP051863.
ISApi2 was found in the GR13-type plasmid p2012N21-164-1 from an Acinetobacter pittii, where it is inserted immediately adjacent to a pdif site and flanked by a 5 bp target site duplication.
Additionnal Accession numbers : CP033536, CP033535, CP051863.
References
1] Robert Moran (2021) Direct submission.