ISAps1
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP084300 | ND | Acinetobacter pseudolwoffii | Acinetobacter pseudolwoffii Acinetobacter pseudolwoffii plasmid pXMC5X702-tetX-145k Acinetobacter johnsonnii plasmid pR4WN_E10B Acinetobacter sp. plasmid pBspH2 |
DNA section
IS Length : 2593 bp
Ends
IR Length : 20/21
IRL : GTAAGCGTCCGCTGAACCCCACTTTTTCTAACTCATTAATACCGCGATAG
IRR : GTAAGCGTGCGCTGAACCCCATAGCTATTTTTTAATTTTGGTCGGGTTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATTCAAGAA | GCTTTACA | AGGGAATATT | 8 |
| GAATTAGATT | ACACAACT | GAAGTTAAAA | 8 |
| CTATGGCCAT | GCTTTATC | ACGGTTAAAG | 8 |
| AGCACAGGCA | GGTTAAGT | ATAAAAATAA | 8 |
DNA sequence
GTAAGCGTCCGCTGAACCCCACTTTTTCTAACTCATTAATACCGCGATAGTGTCCACTAAATTTAAGTGGACACCTATTTATGGATTTAAAGACAGTTAT
AGACAGTACAGCAACGCTAAAAAAGCCCCGTCGAACCTTCACGGCTGAATTTAAACATCAACTTATTCAGCAATGCCAGCAGCCAGATACATCGGTGGCT
AAGGTAGCAATGCAACATCAGGTCAATGCGAATCTGTTGCATAAATGGATTCGTCAGTCCAGATCAATGCCCTCTGCATTAACACCCCCCTCCATTCCAC
AGACTGACTTTCTTCCGGTCATTCTTCACCCGACTCCAGTCAAACAAGAAGCACCTCCGCCATCTGTGCCGGAGAAGAAAGCTGTCGCTCATATCAGAAT
TCCACTGCATCAGAAACAATGTTCCGCAAGAGATCAGGTGATCGAAATCGAATGGCCAGTGGAGTCTGCAACTGAATTACTGTTACTCATCCAAGGTTTG
ACTCAATGATCCATATAAATGAAATCTGGCTCTCTACCCAGCCGATGGATATGCGTGCGGGTATGGATACTGCCATGGCTCAGGTGGTGAGAGCCTTTGG
TTACATCAAACCGCATTGCGCTTACCTGTTCTGTAATAAACGTGGCCATCGCATGAAAGTGCTGGTGCATGATGGATTGGGCATCTGGCTGTGTGCCCGG
CGGCTGGAACAGGGGAAATTTCACTGGGCGCAGGTTCATCAGGGTGAAACCGTAGCCCTCAGTCCGGAACAGTTACAGGCATTGATCCAGGGTTTGCCCT
GGCAGCGCATTGGACGACAGCAGGTGGTGACTATGCTCTAAACCAGGCTCGTGCATTCTGCTATTCTCCAAACGTTCTATTTCCTCTGCATCATGACCTC
AGGCATACTGCGGTCATGAATACGCTGCCTGATTTAAGCCAACTGACCTATGAGCAACTGCTGGAATTTACCAGACAGTTGGCGATTCAGCATCAGTCTC
TAGCGCAATCAAATCAACAATTAGATACCAAGATTCAACATCTAGAAGTATCAAATCAACAATTAGATGCCAAAGTTCAACATCTTGAAGTAACCAACCA
ACATCTTTCCATTCTCACGCAAAAATACGAGCATGAGCTCGCACTATTTAAACAGCACAAATTCGGCAGTAAAAACGAACATCTAACAGCAAAACAAATC
CATCTGTGGGATGAAGCGGTTGAAGAAGATATCGCAGCAGTCGATTTGGAACTGGAACGGCTGAATGCAGATAAAATCGATGCAGCGACACAGAAAGCTC
TCGTCAACAAACCTAAACGTCGACCGCTCCCAGATCATCTACTCACTCTCCGTATTGAGCATGAACCTGCATCAACCCAATGTGCTTGTGGATGCCAACT
GCGTCGTATCGGTGAAGATGTCAGTGAAAAACTGAATTTTAGACCGGCACAGTTCTATAAGGAACAGCATGTACGTGGTAAATGGGTCTGTGATCAGTGC
GACACCCTGACTCAGCAAGCGATGCCTGCTTATGTGATTGATAAAGGCATTGCCAGTCCTGAACTGCTTAGCCATGTGCTGGTGTCCAAGTATGCCGATC
ATTTACCGCTGTACCGTCAACGTCAAATCTTTCTACGCGCAGGTATTGATCTGTCCAGATCCACATTATCTGACTGGATTGGTCGCTGCGGGGTGGAACT
TGAACCCTTGACGGATGTCTTAAAACAGGTGGTGCTGCAGCAGCGGGTGATCCATGCAGATGAAACGCCGGTCACCACCATGCAGCTTGGTGATGATGAA
AAAAAACCGAAAAAAGGTTATGTCTGGGCCTATGCCACGACACAGTACAATCCGGTTCAGGCGGTGATCTATGACTTTCAGCCGAGCCGTTCAGGTCAGC
ATGCTGAAGCCTTCCTGAAAGGCTGGCAGGGTCATTTGGTCTGTGATGATTATAGTGGTTATAAAGCCCGCTTTAGATCAGGCGATGTCATTGAGGTGGG
CTGCATGGCACATGCACGTCGTAAATTCCATGAACTGCATGTGACCGGGAAAAGTCAGATCGCTGAACAGGCATTAGTGCTGATTCAGAAACTGTATGCC
ATAGAAGCAGAACTCAGGAAAAAGACGGATGGTACAACGCAACAACGCTGCGAATACCGGCAACAGCATAGTCAACCGGTGATGCAACAACTGTATGAAT
GGTTAAACCAACATTATCTGACGGTGCCATCGAGTTCTCCAACCGCCAAGGCAATCAATTACAGTCTGAAGCGTTGGCAAGCCTTAAGCCGCTATCTGGA
TGATGGCAATCTGCCCATTGACAATAATTGGGCTGAGAACTCAATGCGCCCCTGGGCCTTGGGACGAAAGAACTGGCTGTTTGCTGGCTCGCTGCGCAGC
GGCCAACGAGCTGCCAATATCATGACTTTAATCCAGTCAGCAAAACTGAATGGCTTGGATCCGTATGCCTATTTAAGTAATGTGCTGAAAAGGTTGCCGA
CACATAAAGTGAGCCAAATAGAAGAATTGCTGCCGCACCGCTGGAAACCCGACCAAAATTAAAAAATAGCTATGGGGTTCAGCGCACGCTTAC
AGACAGTACAGCAACGCTAAAAAAGCCCCGTCGAACCTTCACGGCTGAATTTAAACATCAACTTATTCAGCAATGCCAGCAGCCAGATACATCGGTGGCT
AAGGTAGCAATGCAACATCAGGTCAATGCGAATCTGTTGCATAAATGGATTCGTCAGTCCAGATCAATGCCCTCTGCATTAACACCCCCCTCCATTCCAC
AGACTGACTTTCTTCCGGTCATTCTTCACCCGACTCCAGTCAAACAAGAAGCACCTCCGCCATCTGTGCCGGAGAAGAAAGCTGTCGCTCATATCAGAAT
TCCACTGCATCAGAAACAATGTTCCGCAAGAGATCAGGTGATCGAAATCGAATGGCCAGTGGAGTCTGCAACTGAATTACTGTTACTCATCCAAGGTTTG
ACTCAATGATCCATATAAATGAAATCTGGCTCTCTACCCAGCCGATGGATATGCGTGCGGGTATGGATACTGCCATGGCTCAGGTGGTGAGAGCCTTTGG
TTACATCAAACCGCATTGCGCTTACCTGTTCTGTAATAAACGTGGCCATCGCATGAAAGTGCTGGTGCATGATGGATTGGGCATCTGGCTGTGTGCCCGG
CGGCTGGAACAGGGGAAATTTCACTGGGCGCAGGTTCATCAGGGTGAAACCGTAGCCCTCAGTCCGGAACAGTTACAGGCATTGATCCAGGGTTTGCCCT
GGCAGCGCATTGGACGACAGCAGGTGGTGACTATGCTCTAAACCAGGCTCGTGCATTCTGCTATTCTCCAAACGTTCTATTTCCTCTGCATCATGACCTC
AGGCATACTGCGGTCATGAATACGCTGCCTGATTTAAGCCAACTGACCTATGAGCAACTGCTGGAATTTACCAGACAGTTGGCGATTCAGCATCAGTCTC
TAGCGCAATCAAATCAACAATTAGATACCAAGATTCAACATCTAGAAGTATCAAATCAACAATTAGATGCCAAAGTTCAACATCTTGAAGTAACCAACCA
ACATCTTTCCATTCTCACGCAAAAATACGAGCATGAGCTCGCACTATTTAAACAGCACAAATTCGGCAGTAAAAACGAACATCTAACAGCAAAACAAATC
CATCTGTGGGATGAAGCGGTTGAAGAAGATATCGCAGCAGTCGATTTGGAACTGGAACGGCTGAATGCAGATAAAATCGATGCAGCGACACAGAAAGCTC
TCGTCAACAAACCTAAACGTCGACCGCTCCCAGATCATCTACTCACTCTCCGTATTGAGCATGAACCTGCATCAACCCAATGTGCTTGTGGATGCCAACT
GCGTCGTATCGGTGAAGATGTCAGTGAAAAACTGAATTTTAGACCGGCACAGTTCTATAAGGAACAGCATGTACGTGGTAAATGGGTCTGTGATCAGTGC
GACACCCTGACTCAGCAAGCGATGCCTGCTTATGTGATTGATAAAGGCATTGCCAGTCCTGAACTGCTTAGCCATGTGCTGGTGTCCAAGTATGCCGATC
ATTTACCGCTGTACCGTCAACGTCAAATCTTTCTACGCGCAGGTATTGATCTGTCCAGATCCACATTATCTGACTGGATTGGTCGCTGCGGGGTGGAACT
TGAACCCTTGACGGATGTCTTAAAACAGGTGGTGCTGCAGCAGCGGGTGATCCATGCAGATGAAACGCCGGTCACCACCATGCAGCTTGGTGATGATGAA
AAAAAACCGAAAAAAGGTTATGTCTGGGCCTATGCCACGACACAGTACAATCCGGTTCAGGCGGTGATCTATGACTTTCAGCCGAGCCGTTCAGGTCAGC
ATGCTGAAGCCTTCCTGAAAGGCTGGCAGGGTCATTTGGTCTGTGATGATTATAGTGGTTATAAAGCCCGCTTTAGATCAGGCGATGTCATTGAGGTGGG
CTGCATGGCACATGCACGTCGTAAATTCCATGAACTGCATGTGACCGGGAAAAGTCAGATCGCTGAACAGGCATTAGTGCTGATTCAGAAACTGTATGCC
ATAGAAGCAGAACTCAGGAAAAAGACGGATGGTACAACGCAACAACGCTGCGAATACCGGCAACAGCATAGTCAACCGGTGATGCAACAACTGTATGAAT
GGTTAAACCAACATTATCTGACGGTGCCATCGAGTTCTCCAACCGCCAAGGCAATCAATTACAGTCTGAAGCGTTGGCAAGCCTTAAGCCGCTATCTGGA
TGATGGCAATCTGCCCATTGACAATAATTGGGCTGAGAACTCAATGCGCCCCTGGGCCTTGGGACGAAAGAACTGGCTGTTTGCTGGCTCGCTGCGCAGC
GGCCAACGAGCTGCCAATATCATGACTTTAATCCAGTCAGCAAAACTGAATGGCTTGGATCCGTATGCCTATTTAAGTAATGTGCTGAAAAGGTTGCCGA
CACATAAAGTGAGCCAAATAGAAGAATTGCTGCCGCACCGCTGGAAACCCGACCAAAATTAAAAAATAGCTATGGGGTTCAGCGCACGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 429 bp | 142 aa | 81 | 509 | + | No |
AG : IS66 TnpA
ORF sequence :
MDLKTVIDSTATLKKPRRTFTAEFKHQLIQQCQQPDTSVAKVAMQHQVNANLLHKWIRQSRSMPSALTPPSIPQTDFLPVILHPTPVKQEAPPPSVPEKK
AVAHIRIPLHQKQCSARDQVIEIEWPVESATELLLLIQGLTQ
AVAHIRIPLHQKQCSARDQVIEIEWPVESATELLLLIQGLTQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 336 bp | 111 aa | 506 | 841 | + | No |
AG : IS66 TnpB
ORF sequence :
MIHINEIWLSTQPMDMRAGMDTAMAQVVRAFGYIKPHCAYLFCNKRGHRMKVLVHDGLGIWLCARRLEQGKFHWAQVHQGETVALSPEQLQALIQGLPWQ
RIGRQQVVTML
RIGRQQVVTML
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1647 bp | 548 aa | 916 | 2562 | + | No |
Chemistry : DDE
ORF sequence :
MNTLPDLSQLTYEQLLEFTRQLAIQHQSLAQSNQQLDTKIQHLEVSNQQLDAKVQHLEVTNQHLSILTQKYEHELALFKQHKFGSKNEHLTAKQIHLWDE
AVEEDIAAVDLELERLNADKIDAATQKALVNKPKRRPLPDHLLTLRIEHEPASTQCACGCQLRRIGEDVSEKLNFRPAQFYKEQHVRGKWVCDQCDTLTQ
QAMPAYVIDKGIASPELLSHVLVSKYADHLPLYRQRQIFLRAGIDLSRSTLSDWIGRCGVELEPLTDVLKQVVLQQRVIHADETPVTTMQLGDDEKKPKK
GYVWAYATTQYNPVQAVIYDFQPSRSGQHAEAFLKGWQGHLVCDDYSGYKARFRSGDVIEVGCMAHARRKFHELHVTGKSQIAEQALVLIQKLYAIEAEL
RKKTDGTTQQRCEYRQQHSQPVMQQLYEWLNQHYLTVPSSSPTAKAINYSLKRWQALSRYLDDGNLPIDNNWAENSMRPWALGRKNWLFAGSLRSGQRAA
NIMTLIQSAKLNGLDPYAYLSNVLKRLPTHKVSQIEELLPHRWKPDQN
AVEEDIAAVDLELERLNADKIDAATQKALVNKPKRRPLPDHLLTLRIEHEPASTQCACGCQLRRIGEDVSEKLNFRPAQFYKEQHVRGKWVCDQCDTLTQ
QAMPAYVIDKGIASPELLSHVLVSKYADHLPLYRQRQIFLRAGIDLSRSTLSDWIGRCGVELEPLTDVLKQVVLQQRVIHADETPVTTMQLGDDEKKPKK
GYVWAYATTQYNPVQAVIYDFQPSRSGQHAEAFLKGWQGHLVCDDYSGYKARFRSGDVIEVGCMAHARRKFHELHVTGKSQIAEQALVLIQKLYAIEAEL
RKKTDGTTQQRCEYRQQHSQPVMQQLYEWLNQHYLTVPSSSPTAKAINYSLKRWQALSRYLDDGNLPIDNNWAENSMRPWALGRKNWLFAGSLRSGQRAA
NIMTLIQSAKLNGLDPYAYLSNVLKRLPTHKVSQIEELLPHRWKPDQN
Blast result :
Comments
ISAps1 is 93% aa similar to ISAba17.
ISAps1 is found in the chromosome and in the GR22-type plasmid of Acinetobacter pseudolwoffii isolate XMC5X702 that was isolated from chicken faeces in Jiangsu province, China in 2020. Copies of ISAps1 are also found in plasmids from Acinetobacter johnsonii and Acinetobacter species isolates. All four copies are flanked by 8 bp target site duplications.
Additionnal accession numers : CP084300, CP084302, MT742181, CP055284
ISAps1 is found in the chromosome and in the GR22-type plasmid of Acinetobacter pseudolwoffii isolate XMC5X702 that was isolated from chicken faeces in Jiangsu province, China in 2020. Copies of ISAps1 are also found in plasmids from Acinetobacter johnsonii and Acinetobacter species isolates. All four copies are flanked by 8 bp target site duplications.
Additionnal accession numers : CP084300, CP084302, MT742181, CP055284
References
1] Moran R (2022) Direct submission.