ISAcsp13

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP055284 ND Acinetobacter sp.
Acinetobacter sp.
Acinetobacter sp. plasmid pBspH2
DNA section
IS Length : 2533 bp

Ends


IR Length : 15/16

IRL : GTAAGCGTCCGCTGAACACACCTTATGAATTCATCCTATAAGCCTTAATT
IRR : GTAAGCATCCGCTGAACCCCATGCCTATTTTTTAATTTGAGTTGGATTTC

Insertion site


Left flankDirect repeatRight flankDR Length
TTGGCCCTAATTAATACTTAGTCCTTAA8

DNA sequence

GTAAGCGTCCGCTGAACACACCTTATGAATTCATCCTATAAGCCTTAATTTAGTCCCCACTTAAAAACAAGTGGGGACTAAAATTTATGACTACAAATCA
CCAGACATCCATCGCATCTCTTGCGAAAAAACGAAGAACATACAGTGCTGAATTTAAACAGCAGATCGTTCAGGCTTGTAAAGCACCGGACCTTTCAATT
GCTTCGGTCGCTTTGCAACATGGATTGAATACAAATCTTGTATCCAAATGGATTCGCTTAATTGATGCTAAGCCAGGGAATGATCGCTCACCACTACCGA
ATAAACCTGCATTTATTGCCTTATCCTGCTCTGCACCATTAGATCCTACTCCTAATGACATGTTAACGGTTCAAATTACTTTACCCCACTCAAAAGCAGA
AATTGGCTTGAAATGGCAAGTATCAAAAATATCTGCTTTAGCAGAATTACTCAAGGCACTGGCAACATGATCCGTATCGATGAAATCTGGTTGTCTACCC
AGCCCATGGACATGCGAGCAGGAATGGATACGGCCATGGCTCAGGTGGTGAGAGCCTTTGGCTACATCAAACCGCATTGTGCTTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTGCTGGTACATGATGGACTGGGGATCTGGCTGTGTGCTCGGCGGTTGGAACAGGGAAAATTTCACTGGGCTAAAGTTCAC
CAGGGTGAAAGCGTAGCCCTCAGCCCAGAACAGTTACAGGCACTGATCCAGGGTTTGCCTTGGCAGCGCATTGGACGACAGCAAGTGGTCACAATGCTCT
AAACTAGCTTGGTCCAAGCTGCTATTCTCCAAACGTTCTATTTCATTCTTCTCATGACCTCAGGCATACTGCGGTCATGAATACACTGCCTGATTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTTACTAGACAGTTGGCAATGCAACATCAGTCTCTGGCACAATCAAACCAGCAATTAGATGCCAAAGTTCAGC
ATCTTGAAGTATCCAATCTGCAATTAGATGCCAAAGTTCAGCATCTTTCCATTCTCAATCAAAAATACGAGCATGAACTTGCGCTGTTTAAAAAGCACAA
ATTCGACAGTAAAAACGAACATCTCACCGCAAAACAAATCCATCTCTGGGATGAAGCGGTCGAAGAAGATATTGCCGCTGTTGATCTGGAACTGGAACGG
CTGAATGCAGATAAAACTGATGCAGCGACACACAAAGCCACAGTCAACAAACCTAAACGTCGACCACTGCCAGATCACCTGCCAACCCTCCGTATCGAGC
ATGAACCAACATCAACCCAATGTGCTTGTGGCTGCACCTTGCGTCGTATCGGCGAAGATATCAGTGAAAAACTACATTTCAGACCGGCACAGTTCTATAA
GGAACAGCATGTGCGTGGCAAATGGGTCTGTGATCAGTGTGACACACTGACTCAGCAAGCGATGCCCGCCTATGTGATTGATAAAGGCATTGCCAGTCCT
GAACTGCTTAGCCATGTGTTGGTCTCGAAATACGCAGATCATTTACCGCTGTACCGTCAACGTCTGATCTATCAGCGGGCGGGAATCGAGCTTTCTAGAT
CAACTTTATCTGACTGGATAGGTCGCTGCGGTGTAGAACTGGAACCTCTGGCCAATGCTTTAAAAGAGGTGGTGCTGCAACAGCGGGTGATCCATGCCGA
TGAAACGCCGGTCACCATCATGCGGATGAGTGATGATGAGAAAAAACCGAAGAAAGGTTATGTCTGGGCCTATGCAACAACCCAATATAATCCAATTCAA
GCCGTGATTTATGACTTTCAGGATAGTCGTTCAGGCCAGCATGCTGAAGTGTTCCTGAAAGACTGGCAGGGCCATCTAGTCTGTGATGATTACAGTGGTT
ATAAAGCACGCTTTAAATCAGGCCAAGTCATTGAGGTGGGCTGCATGGCGCATGCTCGTCGTAAATTCCATGAACTGCATGTGACCAAGAAAAGTCAGGT
CGCTGAACAGGCCTTAGTGCTGATTCAGAAACTGTATGCCATAGAAGCAGAACTCAGGAGAAAGACCGATGGTACAGCAGAACAGCGCCGCGAATACCGA
CAACAGCATAGTCAACCGGTGATGCAACAACTGTATGAATGGCTCAACCAGCATCAACTGACGGTGCCATCGAGTTCTCCAACCGCCAAGGCCATCAATT
ACACTCTGAAGCGTTGGCCAGCTTTAAGCCGCTATCTGGATGATGGCAATCTACCTATAGATAATAATTGGGTGGAAAACCAGATGCGACCTTGGGCGTT
GGGACGTAAGAATTGGCTATTTGCAGGTTCGCTGCGCAGTGGACAGCGAGCGGCAAATATCATGACTTTAATCCAGTCAGCAAAGCTGAATGGGTTGGAT
CCGTATGCCTATCTCAGTGATGTGCTGAAAAGGCTGCCGACGCATAAAGCGACCCAAATAGAAGAATTACTACCACATCGCTGGAAATCCAACTCAAATT
AAAAAATAGGCATGGGGTTCAGCGGATGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
384 bp127 aa87470+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MTTNHQTSIASLAKKRRTYSAEFKQQIVQACKAPDLSIASVALQHGLNTNLVSKWIRLIDAKPGNDRSPLPNKPAFIALSCSAPLDPTPNDMLTVQITLP
HSKAEIGLKWQVSKISALAELLKALAT

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
390 bp129 aa413802+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MASIKNICFSRITQGTGNMIRIDEIWLSTQPMDMRAGMDTAMAQVVRAFGYIKPHCAYLFCNKRGHRMKVLVHDGLGIWLCARRLEQGKFHWAKVHQGES
VALSPEQLQALIQGLPWQRIGRQQVVTML

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1626 bp541 aa8772502+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNTLPDLSQLTHEQLLEFTRQLAMQHQSLAQSNQQLDAKVQHLEVSNLQLDAKVQHLSILNQKYEHELALFKKHKFDSKNEHLTAKQIHLWDEAVEEDIA
AVDLELERLNADKTDAATHKATVNKPKRRPLPDHLPTLRIEHEPTSTQCACGCTLRRIGEDISEKLHFRPAQFYKEQHVRGKWVCDQCDTLTQQAMPAYV
IDKGIASPELLSHVLVSKYADHLPLYRQRLIYQRAGIELSRSTLSDWIGRCGVELEPLANALKEVVLQQRVIHADETPVTIMRMSDDEKKPKKGYVWAYA
TTQYNPIQAVIYDFQDSRSGQHAEVFLKDWQGHLVCDDYSGYKARFKSGQVIEVGCMAHARRKFHELHVTKKSQVAEQALVLIQKLYAIEAELRRKTDGT
AEQRREYRQQHSQPVMQQLYEWLNQHQLTVPSSSPTAKAINYTLKRWPALSRYLDDGNLPIDNNWVENQMRPWALGRKNWLFAGSLRSGQRAANIMTLIQ
SAKLNGLDPYAYLSDVLKRLPTHKATQIEELLPHRWKSNSN

 

Blast result :Comments : ORF3 is 93.8% identical to the transposase of ISAba49.
Comments
ISAcsp13 is 96% aa similar to ISAba49.
It was found in the Acinetobacter sp. GR13-type plasmid pBspH2.
References
1] R. Moran (2022) direct submission.
2] Moran et al. (2022). bioRxiv doi: 10.1101/2022.01.12.475240.