ISAjo6

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP068198 ND Acinetobacter johnsonii
Acinetobacter johnsonii
Acinetobacter johnsonii plasmid
DNA section
IS Length : 2 bp

Ends


IR Length : 17

IRL : GTAAGCGTCCGCTGAACACACCTTATGAATTCGTCCTATAAGCCTTAATT
IRR : GTAAGCGTCCGCTGAACCCCATGCCTATTTTTTAATTTGAGTTGGATTTC

Insertion site


Left flankDirect repeatRight flankDR Length
CACATTTGTTGTTAAGCCTTCAGTACTA8
AGAAAACAGAGCATTTATCAGCTCAAAC8
CAGATAACTCTCAGTTTCCACAATCAAT8

DNA sequence

GTAAGCGTCCGCTGAACACACCTTATGAATTCGTCCTATAAGCCTTAATTTAGTCCTGACTTAAAAACAAGTGGGAACTTAAATTTATGACTACAAATAA
CCAGACATCCATCGCATCTCTTACGATAAAACGAAGAACATATAGTGCTGAATTTAAACAGCAGATCGTTCAGGCGTGTAAAGCATCGGAGGTTTCAATT
GCTTCGGTCGCTTTGCAACATGGATTGAATACAAATCTTGTATCCAAATGGATTCGCTTAATTGATGCTAAGCCAGGGAATGATCGCTCACTACTACCGA
ATAAACCTGCATTTATTACCTTATCTTGCTCCGCACCATTAGATCCTATCCCTACTGGCATGTTAACGGTTCAAATTACTTTACCCCACTCAAAAGCAGA
AATTGGCTTGAAATGGCAAGTATCAGAAATATCTGCTTTAGCAGAATTACTCAAGGCACTTACAACATGATCCGTATCGATGAAATCTGGTTGTCTACTC
AGCCCATGGACATGCGTGCAGGTATGGATACGGCCATGGCTCAGGTGGTGAGAGCCTTTGGCTACATCAAACCGCATTGTGCTTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTGCTGGTACATGATGGACTGGGCATCTGGCTGTGTGCCCGGCGGTTGGAACAGGGAAAATTTCACTGGGCTAAAGTTCAC
CAGGGTGAAAGCGTAGCCCTCAGCCCAGAACAGTTACAGGCACTGATCCAGGGTTTGCCTTGGCAGCGCATTGGACGACAGCAGGTGGTGACGATGCTCT
AAACTAGCTTGGTCCATTCTGCTATTCTCCAAACGTTCTATTTCCTCTGCGTCATGACCTCAGGCATACTGCGGTCATGAATACGCTGCCTGATTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTTACCCGGCAGTTGGCAATGCAGCATCAGTCTTTGGCACAATCAAATCAAGAATTAGAAAAATCAAACCAGC
AATTGGATGCCAAAGTTCAACATCTTTCCATTCTCACGCAAAAATACGAGCATGAGCTCGCACTATTTAAACAGCACAAATTTGGCAGTAAAAACGAACA
TCTCACCGCAAAACAAATCCATCTGTGGGATGAAGCGGTCGAAGAAGATATTGCCGCTGTTGATCTGGAACTGGAACGGCTGAATGCAGATAAAACTGAT
GCAGCGACACACAAAGCCACAGTCAACAAACCTAAACGTCGACTGCTGCCCGATCATCTACACACCATCCGTATCGAGCATGAACCAACATCAACCCAAT
GTGCTTGTGGCTGCACCTTGCGTCGTATCGGCGAAGATATCAGTGAAAAACTACATTTCAGACCGGCACAGTTCTATAAGGAACAGCATGTGCGTGGCAA
ATGGGTCTGTGATCAGTGTGACACCCTGACTCAGCAAGCGATGCCTGCCTATGTGATTGATAAAGGCATTGCTTCACCTGAACTGCTCAGCCATGTGCTG
GTATCGAAGTATGCCGATCATTTACCGCTGTACCGTCAACGTCTGATCTATCAGCGGGCGGGAATCGAGCTTTCTAGATCAACGTTATCTGACTGGATAG
GTCGCTGCGGTGTGGAACTGGAACCTCTGGCCAATGCCTTAAAACAGGTGGTGCTGCAACAGCGGGTGATCCATGCCGATGAAACGCCGGTCACCATCAT
GCGGATGGGTGATGATGAGAAAAAACCGAAGAAAGGTTATGTCTGGGCCTATGCCACTACACAGTACAATCCAGTTCAAGCGGTGATCTATGACTTTCAG
GATAGCCGTTCAGGTCAGCATGCAGAAGAGTTCCTGAATGGCTGGCAGGGCCATCTGGTCTGTGATGATTACAGTGGTTATAAAGCACGCTTTAAATCAG
GCGATGTCATAGAGGTGGGCTGCATGGCGCATGCACGTCGTAAATTCCATGAGCTACATGTGACCGGGAAAAGTCAGATCGCTGAACAGGCATTACTGAT
GATTCAGAAACTGTATGCGATAGAAGCAGAACTCAGGAAAAAGACCGATGGTACAGCAGAACACCGCCGCGAATACCGACAACAGCACAGCCAACCGGTG
ATGCAACAACTGTGTGAATGGCTCAACCAACATTATCTGACGGTGCCATCGAGTTCTCCAACCGCCAAGGCCATCAATTACAGTCTGAAGCGTTGGCCAG
CCTTAAGCCGCTATCTGGATGATGGCAATCTACCCATTGACAATAACTGGGCAGAGAACTCAATGCGTCCCTGGGCATTGGGCCGTAAGAACTGGTTGTT
TGCAGGTTCGCTGCGCAGTGGACAGCGAGCGGCAAATATCATGACTTTAATCCAGTCAGCAAAGCTGAATGGGTTGGATCCGTATGCCTATCTCAGTGAT
GTGCTGAAAAGGCTGCCGACGCATAAAGCGACCCAAATAGAAGAATTACTACCACATCGCTGGAAATCCAACTCAAATTAAAAAATAGGCATGGGGTTCA
GCGGACGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
384 bp127 aa87470+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MTTNNQTSIASLTIKRRTYSAEFKQQIVQACKASEVSIASVALQHGLNTNLVSKWIRLIDAKPGNDRSLLPNKPAFITLSCSAPLDPIPTGMLTVQITLP
HSKAEIGLKWQVSEISALAELLKALTT

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
390 bp129 aa413802+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MASIRNICFSRITQGTYNMIRIDEIWLSTQPMDMRAGMDTAMAQVVRAFGYIKPHCAYLFCNKRGHRMKVLVHDGLGIWLCARRLEQGKFHWAKVHQGES
VALSPEQLQALIQGLPWQRIGRQQVVTML

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1605 bp534 aa8772481+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNTLPDLSQLTHEQLLEFTRQLAMQHQSLAQSNQELEKSNQQLDAKVQHLSILTQKYEHELALFKQHKFGSKNEHLTAKQIHLWDEAVEEDIAAVDLELE
RLNADKTDAATHKATVNKPKRRLLPDHLHTIRIEHEPTSTQCACGCTLRRIGEDISEKLHFRPAQFYKEQHVRGKWVCDQCDTLTQQAMPAYVIDKGIAS
PELLSHVLVSKYADHLPLYRQRLIYQRAGIELSRSTLSDWIGRCGVELEPLANALKQVVLQQRVIHADETPVTIMRMGDDEKKPKKGYVWAYATTQYNPV
QAVIYDFQDSRSGQHAEEFLNGWQGHLVCDDYSGYKARFKSGDVIEVGCMAHARRKFHELHVTGKSQIAEQALLMIQKLYAIEAELRKKTDGTAEHRREY
RQQHSQPVMQQLCEWLNQHYLTVPSSSPTAKAINYSLKRWPALSRYLDDGNLPIDNNWAENSMRPWALGRKNWLFAGSLRSGQRAANIMTLIQSAKLNGL
DPYAYLSDVLKRLPTHKATQIEELLPHRWKSNSN

 

Blast result :
Comments
ISAjo6 is 97% aa similar (transposase) to ISAba46.
Additionnal GenBank accessions numbers : CP068198, CP068195, CP065666.
References
1.Haiyang Liu (2022) Direct submission.