ISAba66
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP042558 | ND | Acinetobacter baumannii | Acinetobacter baumannii Acinetobacter sp. plasmid pBspH2 Acinetobacter baumannii plasmid pE47_002 |
DNA section
IS Length : 1323 bp
Ends
IR Length : 26
IRL : TGAACCGTACCGGGTTTGTCGGAGACCATTTTTCTTGAGAGAATGTCCCA
IRR : TGAACCGTACCGGGTTTGTCGGAGACTTTTTGTTTAAGTTAGGCCACCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAGAGTATAT | AAA | TGACGATGCT | 3 |
| AAGGACAAGCATCGTCA | TTT | TATATACTCTAGGA | 3 |
DNA sequence
TGAACCGTACCGGGTTTGTCGGAGACCATTTTTCTTGAGAGAATGTCCCAATGACAAAACCAAAATATACTCCTGAAATCAGAGACAGAGCGGTTCAATT
ATTGATTGAATCCGAAAAAGATTATCCTTCTACTTGGGCTGCAATCACAGCAATTGCACCTAAAATTGGTTGTACCCCTGAAACACTACGTTCCTGGCAT
CAAAAATATTTGGATCAACAAAATCCAGTTAAAGTACAGCAGCTTTCAGATCAAGAACGTATTAAACAGCTCGAACGTGAAAATAAAGAGCTGCAACGTG
CCAATGAAATTCTACGCAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGATGGATTTTATCCATAATAATAAAGAGCCG
TACGGAGTCGAGGCGATTTGTAGGATTTTACCTATCGCACCTTCAACTTATTACCGGACTCTAGATCTCTGCGATGAGAAGCATTGCTTCGCAAGGGAAC
ATCGAGCGAAACGAGATCTACATGATCAACATCATGCTGAACAGATTAAACGTATTTGGAAAGAAAGTTCAGGTCGTTATGGTGTGCGCAAAGTCTGGCA
AAAACTAAGACACGAAGGCTATGTTATTGCACGTTGTACAGTTGCTCGATTGATGCAACAGCTAGGTATACAAGGTGTTTGGCGTGGTAAGAATAAACAA
ACAACGCGTAGCCGAGATGATCAAAAATCAGCAGATGATTTAGTAAAACGCAATTTTAAAGCTGATCATCCTGATCACTTGTGGGTGGCCGACTTCACGT
ATATTCAAACCAATTCAGGCTGGGTCTATACCGCATTTATTATTGATGTGTTCTCACGAGCAATTGTTGGATGGAAAGTATCAACACGGATGAATACCGA
CATGGTGCTCGCTGCACTTGAGCAGGCATTGCATGATCGAGGAATGCCAAATAACGTGATTCATCATAGTGACAGAGGCGTACAGTATCTTTCAATTCGC
TATACCAACCGTTTAGAAGCAGCGAATTTACGAGCATCAGTCGGCACTACGGGGGATTCATACGATAATGCATTGGCTGAAACAGTGAATGGGTTGTACA
AAACAGAGGTGATTGAATATTTAAAAGCAGATTGGCGTGGTTTGGGTGATATACAACTTGCCACATTAAATTTGGTAGATTGGTTCAATAAAGAACGTAT
ACATAGTTCACTAGGTTATGTATCACCTTTTGATTTTGAAGCGATGTACTATGATAAAATTAACCCGTTAGGTCAGGTGGCCTAACTTAAACAAAAAGTC
TCCGACAAACCCGGTACGGTTCA
ATTGATTGAATCCGAAAAAGATTATCCTTCTACTTGGGCTGCAATCACAGCAATTGCACCTAAAATTGGTTGTACCCCTGAAACACTACGTTCCTGGCAT
CAAAAATATTTGGATCAACAAAATCCAGTTAAAGTACAGCAGCTTTCAGATCAAGAACGTATTAAACAGCTCGAACGTGAAAATAAAGAGCTGCAACGTG
CCAATGAAATTCTACGCAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGATGGATTTTATCCATAATAATAAAGAGCCG
TACGGAGTCGAGGCGATTTGTAGGATTTTACCTATCGCACCTTCAACTTATTACCGGACTCTAGATCTCTGCGATGAGAAGCATTGCTTCGCAAGGGAAC
ATCGAGCGAAACGAGATCTACATGATCAACATCATGCTGAACAGATTAAACGTATTTGGAAAGAAAGTTCAGGTCGTTATGGTGTGCGCAAAGTCTGGCA
AAAACTAAGACACGAAGGCTATGTTATTGCACGTTGTACAGTTGCTCGATTGATGCAACAGCTAGGTATACAAGGTGTTTGGCGTGGTAAGAATAAACAA
ACAACGCGTAGCCGAGATGATCAAAAATCAGCAGATGATTTAGTAAAACGCAATTTTAAAGCTGATCATCCTGATCACTTGTGGGTGGCCGACTTCACGT
ATATTCAAACCAATTCAGGCTGGGTCTATACCGCATTTATTATTGATGTGTTCTCACGAGCAATTGTTGGATGGAAAGTATCAACACGGATGAATACCGA
CATGGTGCTCGCTGCACTTGAGCAGGCATTGCATGATCGAGGAATGCCAAATAACGTGATTCATCATAGTGACAGAGGCGTACAGTATCTTTCAATTCGC
TATACCAACCGTTTAGAAGCAGCGAATTTACGAGCATCAGTCGGCACTACGGGGGATTCATACGATAATGCATTGGCTGAAACAGTGAATGGGTTGTACA
AAACAGAGGTGATTGAATATTTAAAAGCAGATTGGCGTGGTTTGGGTGATATACAACTTGCCACATTAAATTTGGTAGATTGGTTCAATAAAGAACGTAT
ACATAGTTCACTAGGTTATGTATCACCTTTTGATTTTGAAGCGATGTACTATGATAAAATTAACCCGTTAGGTCAGGTGGCCTAACTTAAACAAAAAGTC
TCCGACAAACCCGGTACGGTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 105 aa | 51 | 368 | + | No |
Description : First part of the transposase
ORF sequence :
MTKPKYTPEIRDRAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRSWHQKYLDQQNPVKVQQLSDQERIKQLERENKELQRANEILRKAAAFFAQAEL
DRPHK
DRPHK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 978 bp | 325 aa | 308 | 1285 | + | No |
Description : Second part of the transposase
ORF sequence :
MMDFIHNNKEPYGVEAICRILPIAPSTYYRTLDLCDEKHCFAREHRAKRDLHDQHHAEQIKRIWKESSGRYGVRKVWQKLRHEGYVIARCTVARLMQQLG
IQGVWRGKNKQTTRSRDDQKSADDLVKRNFKADHPDHLWVADFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLAALEQALHDRGMPNNVIHH
SDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWRGLGDIQLATLNLVDWFNKERIHSSLGYVSPFDFEAMYYDKINP
LGQVA
IQGVWRGKNKQTTRSRDDQKSADDLVKRNFKADHPDHLWVADFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLAALEQALHDRGMPNNVIHH
SDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWRGLGDIQLATLNLVDWFNKERIHSSLGYVSPFDFEAMYYDKINP
LGQVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1235 bp | 411 aa | 51 | 1285 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKPKYTPEIRDRAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRSWHQKYLDQQNPVKVQQLSDQERIKQLERENKELQRANEILRKAAAFFRPGGA
RPPTQIMMDFIHNNKEPYGVEAICRILPIAPSTYYRTLDLCDEKHCFAREHRAKRDLHDQHHAEQIKRIWKESSGRYGVRKVWQKLRHEGYVIARCTVAR
LMQQLGIQGVWRGKNKQTTRSRDDQKSADDLVKRNFKADHPDHLWVADFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLAALEQALHDRGMP
NNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWRGLGDIQLATLNLVDWFNKERIHSSLGYVSPFDFEAMY
YDKINPLGQVA
RPPTQIMMDFIHNNKEPYGVEAICRILPIAPSTYYRTLDLCDEKHCFAREHRAKRDLHDQHHAEQIKRIWKESSGRYGVRKVWQKLRHEGYVIARCTVAR
LMQQLGIQGVWRGKNKQTTRSRDDQKSADDLVKRNFKADHPDHLWVADFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLAALEQALHDRGMP
NNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWRGLGDIQLATLNLVDWFNKERIHSSLGYVSPFDFEAMY
YDKINPLGQVA
Blast result :
Comments
ISAba66 is 95% aa similar to ISAlw4.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAba66 was found in the GR13-type plasmids pE47_002 and pBspH2 from A. baumannii E47 and an Acinetobacter species isolate, respectively. It is in the same context in both plasmids, flanked by a 3 bp target site duplication.
Additionnal accession number : CP055284.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAba66 was found in the GR13-type plasmids pE47_002 and pBspH2 from A. baumannii E47 and an Acinetobacter species isolate, respectively. It is in the same context in both plasmids, flanked by a 3 bp target site duplication.
Additionnal accession number : CP055284.
References
1] R. Moran (2022) Direct submission.