ISAba68

  • Family IS5
  • Group IS903
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP041149 ND Acinetobacter baumannii
Acinetobacter baumannii
Acinetobacter pittii
Acinetobacter baumannii plasmid pCUVET596
Acinetobacter baumannii CUVET-MIC596
DNA section
IS Length : 1039 bp

Ends


IR Length : 16

IRL : GGCTTTGTTGCACAAACCTATCTCTAAAGGCTTATTCCACAATATAATTC
IRR : GGCTTTGTTGCACAAAGATTTGAAATGCAAAGCGCCCCTAATTGAGCCAA

Insertion site


Left flankDirect repeatRight flankDR Length
GTGTGTTGCTGTAGGTAACACTAAACCTT9
CAAAAAACACGCTATATAGTAAAAAGACA9
ACATCTTCATAATCATAAGT0

DNA sequence

GGCTTTGTTGCACAAACCTATCTCTAAAGGCTTATTCCACAATATAATTCTCAAATGAATAAGCCCACACCTAAAATCTATCGTACAACCAATTGGTCTT
CTTATAACAGTGCATTAATAAATCGAGGAAATCTCTCAATTTGGTTTGATCCCAAGACTCAATGGTACGCTCAACCCAAAGGTAAACATGGTCGAAATCA
AACTTATTCAGATACAGCCATCCAATGCTGTTTAATGATCAAATCCTTATTTCGTCTTTCTTTACGCATGGTCACTGGCTTTGTGCAAAGTCTCATTCAT
CTTTGTAGATTAGATTGGACAGCACCGGATTATTCAACCATTTGTAGAAGACAAAAGCATATTGATATTGCAATTAACTATCAAAAAAGCAGTAATGGTC
TCCAGCTACTCGTCGATTCTACTGGCTTAAAGTTTCTAGGCGAAGGTGAATGGAAGCGTAAGAAACACCAACCTGAATATCGTCGCCAATGGCGTAAACT
ACATATTGGTATAGATGCCAAAACCCTACAAATACGCGCTATTCAGCTCACAACCAATAATGTCAGTGATTCACAGGTGCTTGGTGATTTACTTAATCAG
ATTCCACAAGATGAGCAGATTGACTCTGTTTATACCGATGGAGCTTATGACACCAAGCAATGCCGTCAGGTCATTGCAGATCGGCAAGCGCATGCGGTGA
TTCCACCTAGAAAAAATGCGAAACCATGGAAAGATACAAAGATCAGCTCTCTAGAGCGAAATGAATTACTTCGAACAGTTAAACGTTTAGGCAGGACATT
ATGGAAAAAATGGTCAGGCTATCATCGTCGAAGTTTGGTTGAAACTAAGATGCATTGCATCAAATTATTAGGAGATAAACTCAGTGCAAGGAGTTTTGAT
AGCCAAGTGAATGAGATCCATGCACGTGTAGCAGTCCTTAACAGATTTACGGAATTAGGTCGACCACTTACCCAAGTTACGCCTTAAATTTGGCTCAATT
AGGGGCGCTTTGCATTTCAAATCTTTGTGCAACAAAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
933 bp310 aa55987+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNKPTPKIYRTTNWSSYNSALINRGNLSIWFDPKTQWYAQPKGKHGRNQTYSDTAIQCCLMIKSLFRLSLRMVTGFVQSLIHLCRLDWTAPDYSTICRRQ
KHIDIAINYQKSSNGLQLLVDSTGLKFLGEGEWKRKKHQPEYRRQWRKLHIGIDAKTLQIRAIQLTTNNVSDSQVLGDLLNQIPQDEQIDSVYTDGAYDT
KQCRQVIADRQAHAVIPPRKNAKPWKDTKISSLERNELLRTVKRLGRTLWKKWSGYHRRSLVETKMHCIKLLGDKLSARSFDSQVNEIHARVAVLNRFTE
LGRPLTQVTP

 

Blast result :
Comments
ISAba68 is 98% aa similar to ISAha1.
ISAba68 was found in the GR13-type plasmid of A. baumannii CUVET-MIC596. A copy of ISAba68 is also present in the CUVET-MIC596 chromosome. Both copies are flanked by 9 bp target site duplications.
Additionnal GenBank accession number : CP041148 and CP027254.
References
1] Moran R (2022) Direct submission.