ISAjo4
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP079794 | ND | Acinetobacter johnosonii | Acinetobacter johnosonii plasmid pAJ_082-3 Acinetobacter johnosonii |
DNA section
IS Length : 2512 bp
Ends
IR Length : 16
IRL : GTAAGCGTCCGCTGAACACACCTTATGAATTCATCCTATAAGCCTTAATT
IRR : GTAAGCGTCCGCTGAATCCCATGCCTATTTTTTAATTTTGGTCGGATTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGGTAAACAT | GAACAAGA | AGCTGCTAGG | 8 |
| GGTGTGCTGT | CTATAACT | GTCTTTAAAT | 8 |
DNA sequence
GTAAGCGTCCGCTGAACACACCTTATGAATTCATCCTATAAGCCTTAATTTAGTCCCCACTTAAAAACTAGTGGGGACTAAAATTTATGACTACAAATCA
CCAGACATCCATCGCATCTCTTGCGAAAAAACGAAGAACATACAGTGCTGAATTTAAACAGCAGATCGTTCAGGCTTGTAAAGCACCGGACGTTTCAATT
GCTTCGGTCGCTTTGCAACATGGATTGAATACAAATCTTGTATCCAAATGGATTCGCTTAATTGATGGTAAGCCAGGGAATGATCGCTCACCACTACCGA
ATAAACCTGCATTTATTGCCTTATCCTGCTCTGCACCATTAGATCCTACTCCTACTGACATGTTAACGGTTCAAATTACTTTACCCCACTCAAAAGCAGA
AATTGGCTTGAAATGGCAAGTATCAGAAATATCTGCTTTAGCAGAATTACTCAAGGCACTTGCAACATGATCCGCATTGATGAAATCTGGCTTTCTACCC
AACCTCTGGATATGCGAGCAGGGATGGATACTATCATGGCTCAGGTGGTGAGAGCCTTTGGCTACATTAAACCGCATTGTGCTTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTGCTGGTACATGATGGACTGGGCATCTGGCTGTGTGCCCGGCGGTTGGAACAGGGCAAATTTCACTGGGCTAAAGTTCAC
CAGGGTGAAAGCGTAGCCCTCAGCCCGGAACAGTTACAGGCACTGATCCAGGGTTTGCCCTGGCAGCGCATTGGACGACAGCAGGTAGTGACGATGCTCT
AAACCAGGTTGGTGCATTCTGCTATTCTCCAAACGTTCTATTTCCTCTGCGTCATGACCTCAGGCATACTGCGGTCATGAATACGCTGCCTGACTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTTACCAGACAGTTGGCAATGCAGCATCAACAACTCGCAGAAGATAACCAACAATTAGATACCAAAGTTCAGC
ATCTTGAAGCATGCAACCAACATCTTTCCATTCTCAATCAAAAATACGAGCATGAACTGGCGCTGTTTAAAAAGCACAAATTCGGCAGTAAAAACGAACA
TCTCACCGCAAAACAAATCCATCTCTGGGATGAAGCGGTCGAAGAAGATATTGCAGCCGTTGATTTGGAACTGGAACGGCTGAATGCAGATAAAACCGAT
GCAGCGACACACAAAGCCAAAGCAAATAAACCTAAACGTCGACTGCTGCCCGATCATCTACACACCATCCGTATTGAGCATGAACCTGCATCAACCCAAT
GCAGTTGTGGCTGCCAGTTACGTCGTATCGGCGAAGATATCAGTGAAAAACTGCATTTCAGACCGGCACAGTTCTATAAGGAACAGCATGTACGTGGCAA
ATGGGTCTGTGATCAGTGTGACACCCTGACTCAGCAAGCGATGCCCGCCTATGTGATTGATAAAGGCATTGCTTCACCTGAACTGCTCAGCCATGTGCTG
GTATCGAAGTATGCCGATCATTTACCGCTGTACCGTCAACGTCTGATCTATCAGCGGGCGGGAATCGAGCTTTCTAGATCAACGTTATCTGACTGGATAG
GTCGCTGCGGTGTAGAACTGGAGCCTCTGGCCAATGCCTTAAAAGAGGTGGTGCTGCAACAGCAGGTGCTGCATGCAGATGAAACACCGGTCACCATCAT
GCGGATGGGTGAGAATGAGAAAAAACCGAAGAAAGGTTATGTCTGGGCCTATGCCACTACACAGTACAATCCAGTTCAGGCGGTGATCTATGACTTTCAG
GATAGCCGTTCAGGTCAGCATGCAGAAGAGTTCCTGAATGGCTGGCAGGGCCATCTGGTCTGTGATGATTACAGTGGTTATAAAGCACGCTTTAAATCAG
GCGATGTCATTGAGGTGGGCTGCATGGCACATGCACGTCGTAAATTTCATGAACTGCATGTGACCGGGAAAAGTCAGATCGCTGAACAGGCATTAGTGCT
GATTCAGAAACTGTATGCCATAGAAGCAGAACTCAGGAAAAAGACCGATGGTACAGCAGAACATCGCCGCGAATATCGGCAAAAGCATAGTCAACCGGTG
ATGCAACAACTGTATGAATGGCTCAACCAACATTATCTGACGGTACCATCGAGTTCTCCAACTGCCAAGGCGATCAATTACAGCCTAAAACGTTGGCAGG
CACTCTGCCGCTATCTGGATGATGGTAATTTGCCTATAGATAATAACTGGATTGAAAATCAGATGCGCCCCTGGGCGTTGGGACGTAAGAACTGGCTGTT
TGCTGGCTCGCTGCGCAGCGGCCAACGAGCTGCCAATATCATGACTTTAATCCAGTCAGCAAAGCTGAATGGACTGGATCCGTATGCCTATTTAAGTGAT
GTGCTGAAAAGGCTGCCGACGCATAAAGTGACCCAAATAGAAGAATTACTGCCACACCGCTGGAAATCCGACCAAAATTAAAAAATAGGCATGGGATTCA
GCGGACGCTTAC
CCAGACATCCATCGCATCTCTTGCGAAAAAACGAAGAACATACAGTGCTGAATTTAAACAGCAGATCGTTCAGGCTTGTAAAGCACCGGACGTTTCAATT
GCTTCGGTCGCTTTGCAACATGGATTGAATACAAATCTTGTATCCAAATGGATTCGCTTAATTGATGGTAAGCCAGGGAATGATCGCTCACCACTACCGA
ATAAACCTGCATTTATTGCCTTATCCTGCTCTGCACCATTAGATCCTACTCCTACTGACATGTTAACGGTTCAAATTACTTTACCCCACTCAAAAGCAGA
AATTGGCTTGAAATGGCAAGTATCAGAAATATCTGCTTTAGCAGAATTACTCAAGGCACTTGCAACATGATCCGCATTGATGAAATCTGGCTTTCTACCC
AACCTCTGGATATGCGAGCAGGGATGGATACTATCATGGCTCAGGTGGTGAGAGCCTTTGGCTACATTAAACCGCATTGTGCTTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTGCTGGTACATGATGGACTGGGCATCTGGCTGTGTGCCCGGCGGTTGGAACAGGGCAAATTTCACTGGGCTAAAGTTCAC
CAGGGTGAAAGCGTAGCCCTCAGCCCGGAACAGTTACAGGCACTGATCCAGGGTTTGCCCTGGCAGCGCATTGGACGACAGCAGGTAGTGACGATGCTCT
AAACCAGGTTGGTGCATTCTGCTATTCTCCAAACGTTCTATTTCCTCTGCGTCATGACCTCAGGCATACTGCGGTCATGAATACGCTGCCTGACTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTTACCAGACAGTTGGCAATGCAGCATCAACAACTCGCAGAAGATAACCAACAATTAGATACCAAAGTTCAGC
ATCTTGAAGCATGCAACCAACATCTTTCCATTCTCAATCAAAAATACGAGCATGAACTGGCGCTGTTTAAAAAGCACAAATTCGGCAGTAAAAACGAACA
TCTCACCGCAAAACAAATCCATCTCTGGGATGAAGCGGTCGAAGAAGATATTGCAGCCGTTGATTTGGAACTGGAACGGCTGAATGCAGATAAAACCGAT
GCAGCGACACACAAAGCCAAAGCAAATAAACCTAAACGTCGACTGCTGCCCGATCATCTACACACCATCCGTATTGAGCATGAACCTGCATCAACCCAAT
GCAGTTGTGGCTGCCAGTTACGTCGTATCGGCGAAGATATCAGTGAAAAACTGCATTTCAGACCGGCACAGTTCTATAAGGAACAGCATGTACGTGGCAA
ATGGGTCTGTGATCAGTGTGACACCCTGACTCAGCAAGCGATGCCCGCCTATGTGATTGATAAAGGCATTGCTTCACCTGAACTGCTCAGCCATGTGCTG
GTATCGAAGTATGCCGATCATTTACCGCTGTACCGTCAACGTCTGATCTATCAGCGGGCGGGAATCGAGCTTTCTAGATCAACGTTATCTGACTGGATAG
GTCGCTGCGGTGTAGAACTGGAGCCTCTGGCCAATGCCTTAAAAGAGGTGGTGCTGCAACAGCAGGTGCTGCATGCAGATGAAACACCGGTCACCATCAT
GCGGATGGGTGAGAATGAGAAAAAACCGAAGAAAGGTTATGTCTGGGCCTATGCCACTACACAGTACAATCCAGTTCAGGCGGTGATCTATGACTTTCAG
GATAGCCGTTCAGGTCAGCATGCAGAAGAGTTCCTGAATGGCTGGCAGGGCCATCTGGTCTGTGATGATTACAGTGGTTATAAAGCACGCTTTAAATCAG
GCGATGTCATTGAGGTGGGCTGCATGGCACATGCACGTCGTAAATTTCATGAACTGCATGTGACCGGGAAAAGTCAGATCGCTGAACAGGCATTAGTGCT
GATTCAGAAACTGTATGCCATAGAAGCAGAACTCAGGAAAAAGACCGATGGTACAGCAGAACATCGCCGCGAATATCGGCAAAAGCATAGTCAACCGGTG
ATGCAACAACTGTATGAATGGCTCAACCAACATTATCTGACGGTACCATCGAGTTCTCCAACTGCCAAGGCGATCAATTACAGCCTAAAACGTTGGCAGG
CACTCTGCCGCTATCTGGATGATGGTAATTTGCCTATAGATAATAACTGGATTGAAAATCAGATGCGCCCCTGGGCGTTGGGACGTAAGAACTGGCTGTT
TGCTGGCTCGCTGCGCAGCGGCCAACGAGCTGCCAATATCATGACTTTAATCCAGTCAGCAAAGCTGAATGGACTGGATCCGTATGCCTATTTAAGTGAT
GTGCTGAAAAGGCTGCCGACGCATAAAGTGACCCAAATAGAAGAATTACTGCCACACCGCTGGAAATCCGACCAAAATTAAAAAATAGGCATGGGATTCA
GCGGACGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 384 bp | 127 aa | 87 | 470 | + | No |
AG : IS66 TnpA
ORF sequence :
MTTNHQTSIASLAKKRRTYSAEFKQQIVQACKAPDVSIASVALQHGLNTNLVSKWIRLIDGKPGNDRSPLPNKPAFIALSCSAPLDPTPTDMLTVQITLP
HSKAEIGLKWQVSEISALAELLKALAT
HSKAEIGLKWQVSEISALAELLKALAT
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 336 bp | 111 aa | 467 | 802 | + | No |
AG : IS66 TnpB
ORF sequence :
MIRIDEIWLSTQPLDMRAGMDTIMAQVVRAFGYIKPHCAYLFCNKRGHRMKVLVHDGLGIWLCARRLEQGKFHWAKVHQGESVALSPEQLQALIQGLPWQ
RIGRQQVVTML
RIGRQQVVTML
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1605 bp | 534 aa | 877 | 2481 | + | No |
Chemistry : DDE
ORF sequence :
MNTLPDLSQLTHEQLLEFTRQLAMQHQQLAEDNQQLDTKVQHLEACNQHLSILNQKYEHELALFKKHKFGSKNEHLTAKQIHLWDEAVEEDIAAVDLELE
RLNADKTDAATHKAKANKPKRRLLPDHLHTIRIEHEPASTQCSCGCQLRRIGEDISEKLHFRPAQFYKEQHVRGKWVCDQCDTLTQQAMPAYVIDKGIAS
PELLSHVLVSKYADHLPLYRQRLIYQRAGIELSRSTLSDWIGRCGVELEPLANALKEVVLQQQVLHADETPVTIMRMGENEKKPKKGYVWAYATTQYNPV
QAVIYDFQDSRSGQHAEEFLNGWQGHLVCDDYSGYKARFKSGDVIEVGCMAHARRKFHELHVTGKSQIAEQALVLIQKLYAIEAELRKKTDGTAEHRREY
RQKHSQPVMQQLYEWLNQHYLTVPSSSPTAKAINYSLKRWQALCRYLDDGNLPIDNNWIENQMRPWALGRKNWLFAGSLRSGQRAANIMTLIQSAKLNGL
DPYAYLSDVLKRLPTHKVTQIEELLPHRWKSDQN
RLNADKTDAATHKAKANKPKRRLLPDHLHTIRIEHEPASTQCSCGCQLRRIGEDISEKLHFRPAQFYKEQHVRGKWVCDQCDTLTQQAMPAYVIDKGIAS
PELLSHVLVSKYADHLPLYRQRLIYQRAGIELSRSTLSDWIGRCGVELEPLANALKEVVLQQQVLHADETPVTIMRMGENEKKPKKGYVWAYATTQYNPV
QAVIYDFQDSRSGQHAEEFLNGWQGHLVCDDYSGYKARFKSGDVIEVGCMAHARRKFHELHVTGKSQIAEQALVLIQKLYAIEAELRKKTDGTAEHRREY
RQKHSQPVMQQLYEWLNQHYLTVPSSSPTAKAINYSLKRWQALCRYLDDGNLPIDNNWIENQMRPWALGRKNWLFAGSLRSGQRAANIMTLIQSAKLNGL
DPYAYLSDVLKRLPTHKVTQIEELLPHRWKSDQN
Blast result :
Comments
ISAjo4 is 95% aa (transposase) similar to ISAba49.
ISAjo4 was found in the GR13-type plasmid pAJ_082-3 of A. johnsonii AJ_082, which was isolated from a hospital sink in Pakistan in 2016. A copy of ISAjo4 that differs by 10 nucleotides from the one in pAJ_082-3 is present in the chromosome of AJ_082. Both copies of ISAjo4 are flanked by 8 bp target site duplications.
Additionnal accession number : CP079792.
ISAjo4 was found in the GR13-type plasmid pAJ_082-3 of A. johnsonii AJ_082, which was isolated from a hospital sink in Pakistan in 2016. A copy of ISAjo4 that differs by 10 nucleotides from the one in pAJ_082-3 is present in the chromosome of AJ_082. Both copies of ISAjo4 are flanked by 8 bp target site duplications.
Additionnal accession number : CP079792.
References
1] Moran R (2022) Direct submission .