ISAjo3
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP010358 | ND | Acinetobacter johnsonii | Acinetobacter baumannii Acinetobacter johnsonii Acinetobacter johnsonii plasmid Acinetobacter johnsonii plasmid pXBB1-8 |
DNA section
IS Length : 1186 bp
Ends
IR Length : 14/17
IRL : GGTAATCCCCCCTAAAAATAAAAGGATCTAAAAGTAGAATTTTCTCTTAA
IRR : GGTAATCCTCCCATAAATAACCGAAGTTAAAAGTAGAGGTTAAGCAGCCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGTTGGGTG | ATAACTTAAA | 0 | |
| TTATAGCTCC | ATAACTTAAA | 0 | |
| TTAGGAGATG | CAAG | CTATTGCAGA | 4 |
| AATTTAACAG | GAAC | ACCGTTCTCG | 4 |
DNA sequence
GGTAATCCCCCCTAAAAATAAAAGGATCTAAAAGTAGAATTTTCTCTTAAGATACGAGCAGGAGATTCTGCATGAAAACATCTAAATTTACTGACAGCCA
GATCATGTCCATTTTAAAACAGGCGGAGTCTGGCACACCTGTAGCCACCCTTTGTCGTGAACATGGCATGAGTAATGCCACGTTTTATAAATGGCGTGCC
AGATATGGCGGTATGGATACCTCATTGATGGCTCGACTGAAAGAGCTGGAAGCAGAAAATACCAGACTAAAAAAAATGTATGCTGAAGAGCGATTAAAGG
CAGAGATTGTTCAGGAAGCAATGTCAAAAAAGTGGTGAAGCCATCTTGCCGACGTCAGATGGCTCAACAAGCCGTTGCCCTGCATGCGGTTAGTATCCGT
TTAGCTTGCTCTGCATTTGGCATTAGTGAAACCTGCTACCGTTATCAGGCTAAACTTTGCGATGACAATGCATTAATTGCTAAACAGCTCATTGAACTCA
CTGAGGCAAATTCCGATTGGGGATTTGGTCTGTGCTTCTCATATTTACGACATGTTGAGAATCACTGCTGGAATCACAAGCGGGTTTACCGCATTTACTG
TGAGTTGGCACTAAATCTGCGTATTAAACCGAGAAGAAGACTAAAACGGCACGCGCCTGAACCATTGAAAGAACCAATCCGAGAAAATCAGGTCTGGTCA
TTAGATTTTATGCATGATCAGCTGATCGATGGTCGAAAGTTCCGATTATTAAATGTGATTGATGACTACCGTCGAGAAGGCTTAGCGATTGAAGCAGGAT
TCTCATTACCTACAATCCGAGTTATTCGTACGTTGAATCAGTTGCTGGAGTGGCGAGAAAAGCCGTTAGTTATTCGGTGTGACAATGGCCCGGAGTTTAT
CAGTCATGAATTTGTACGCTGGGCTACTGAGCACGGTATTCGTATTGAATATATTCAACCAGGTAAGCCACAGCAGAATGCGTATATTGAACGTTATAAT
CGGACTATACGTTATAGCTGGCTAAGCAAGCATCTATTTGACACACTGGAAGAAGTACAAGATTATGCAACAAACTGGTTGTGGCATTACAACCATGAAA
GACCACATCAAGCTAACAAAGGAAAGCCACCTCTAAGGGCTGCTTAACCTCTACTTTTAACTTCGGTTATTTATGGGAGGATTACC
GATCATGTCCATTTTAAAACAGGCGGAGTCTGGCACACCTGTAGCCACCCTTTGTCGTGAACATGGCATGAGTAATGCCACGTTTTATAAATGGCGTGCC
AGATATGGCGGTATGGATACCTCATTGATGGCTCGACTGAAAGAGCTGGAAGCAGAAAATACCAGACTAAAAAAAATGTATGCTGAAGAGCGATTAAAGG
CAGAGATTGTTCAGGAAGCAATGTCAAAAAAGTGGTGAAGCCATCTTGCCGACGTCAGATGGCTCAACAAGCCGTTGCCCTGCATGCGGTTAGTATCCGT
TTAGCTTGCTCTGCATTTGGCATTAGTGAAACCTGCTACCGTTATCAGGCTAAACTTTGCGATGACAATGCATTAATTGCTAAACAGCTCATTGAACTCA
CTGAGGCAAATTCCGATTGGGGATTTGGTCTGTGCTTCTCATATTTACGACATGTTGAGAATCACTGCTGGAATCACAAGCGGGTTTACCGCATTTACTG
TGAGTTGGCACTAAATCTGCGTATTAAACCGAGAAGAAGACTAAAACGGCACGCGCCTGAACCATTGAAAGAACCAATCCGAGAAAATCAGGTCTGGTCA
TTAGATTTTATGCATGATCAGCTGATCGATGGTCGAAAGTTCCGATTATTAAATGTGATTGATGACTACCGTCGAGAAGGCTTAGCGATTGAAGCAGGAT
TCTCATTACCTACAATCCGAGTTATTCGTACGTTGAATCAGTTGCTGGAGTGGCGAGAAAAGCCGTTAGTTATTCGGTGTGACAATGGCCCGGAGTTTAT
CAGTCATGAATTTGTACGCTGGGCTACTGAGCACGGTATTCGTATTGAATATATTCAACCAGGTAAGCCACAGCAGAATGCGTATATTGAACGTTATAAT
CGGACTATACGTTATAGCTGGCTAAGCAAGCATCTATTTGACACACTGGAAGAAGTACAAGATTATGCAACAAACTGGTTGTGGCATTACAACCATGAAA
GACCACATCAAGCTAACAAAGGAAAGCCACCTCTAAGGGCTGCTTAACCTCTACTTTTAACTTCGGTTATTTATGGGAGGATTACC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 72 | 338 | + | No |
Description : First part of the transposase
ORF sequence :
MKTSKFTDSQIMSILKQAESGTPVATLCREHGMSNATFYKWRARYGGMDTSLMARLKELEAENTRLKKMYAEERLKAEIVQEAMSKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 861 bp | 286 aa | 287 | 1147 | + | No |
Description : Second part of the transposase
ORF sequence :
RAIKGRDCSGSNVKKVVKPSCRRQMAQQAVALHAVSIRLACSAFGISETCYRYQAKLCDDNALIAKQLIELTEANSDWGFGLCFSYLRHVENHCWNHKRV
YRIYCELALNLRIKPRRRLKRHAPEPLKEPIRENQVWSLDFMHDQLIDGRKFRLLNVIDDYRREGLAIEAGFSLPTIRVIRTLNQLLEWREKPLVIRCDN
GPEFISHEFVRWATEHGIRIEYIQPGKPQQNAYIERYNRTIRYSWLSKHLFDTLEEVQDYATNWLWHYNHERPHQANKGKPPLRAA
YRIYCELALNLRIKPRRRLKRHAPEPLKEPIRENQVWSLDFMHDQLIDGRKFRLLNVIDDYRREGLAIEAGFSLPTIRVIRTLNQLLEWREKPLVIRCDN
GPEFISHEFVRWATEHGIRIEYIQPGKPQQNAYIERYNRTIRYSWLSKHLFDTLEEVQDYATNWLWHYNHERPHQANKGKPPLRAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1076 bp | 358 aa | 72 | 1147 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTSKFTDSQIMSILKQAESGTPVATLCREHGMSNATFYKWRARYGGMDTSLMARLKELEAENTRLKKMYAEERLKAEIVQEAMSKKVVKPSCRRQMAQQ
AVALHAVSIRLACSAFGISETCYRYQAKLCDDNALIAKQLIELTEANSDWGFGLCFSYLRHVENHCWNHKRVYRIYCELALNLRIKPRRRLKRHAPEPLK
EPIRENQVWSLDFMHDQLIDGRKFRLLNVIDDYRREGLAIEAGFSLPTIRVIRTLNQLLEWREKPLVIRCDNGPEFISHEFVRWATEHGIRIEYIQPGKP
QQNAYIERYNRTIRYSWLSKHLFDTLEEVQDYATNWLWHYNHERPHQANKGKPPLRAA
AVALHAVSIRLACSAFGISETCYRYQAKLCDDNALIAKQLIELTEANSDWGFGLCFSYLRHVENHCWNHKRVYRIYCELALNLRIKPRRRLKRHAPEPLK
EPIRENQVWSLDFMHDQLIDGRKFRLLNVIDDYRREGLAIEAGFSLPTIRVIRTLNQLLEWREKPLVIRCDNGPEFISHEFVRWATEHGIRIEYIQPGKP
QQNAYIERYNRTIRYSWLSKHLFDTLEEVQDYATNWLWHYNHERPHQANKGKPPLRAA
Blast result :
Comments
ISAjo3 is 97% aa (transposase) similar to ISAca1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAjo3 was found in the A. johnsonii GR13-type plasmid pXBB1-8. Copies flanked by 4 bp target site duplications were found in the chromosome of A. baumannii A388.
Additionnal accession number : CP079793, CP024418.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAjo3 was found in the A. johnsonii GR13-type plasmid pXBB1-8. Copies flanked by 4 bp target site duplications were found in the chromosome of A. baumannii A388.
Additionnal accession number : CP079793, CP024418.
References
1] Moran R (2022) Direct submission.