ISKrh4
- Family IS30
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP097204 | ND | Kocuria rhizophila | Kocuria rhizophila Kocuria rhizophila Noodlococcus |
DNA section
IS Length : 1461 bp
Ends
IR Length : 25/32
IRL : GTGGGTTCTAGCGATCCCCGCAACACCCTGAATTTCAGGGAGTTGCGGGG
IRR : GGCGGTTTCTAGGGGTCCTCGCAACACCTGATGGTTATGTGGTTGTCAGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCACTCTCCC | ACGG | AAGGGTGCGG | 4 |
| GCCTGGTCGG | CGGG | AGGTGAGCGG | 4 |
| CCCCCGGGAG | GGATGGGAGC | 0 | |
| CCCCCGAAAG | CGGGAGGGGA | 0 |
DNA sequence
GTGGGTTCTAGCGATCCCCGCAACACCCTGAATTTCAGGGAGTTGCGGGGATCGTGTTGTCAGATGAGTTGAAGGTGGCGTTTTTCCAGGCGCTGGAACG
TGGGGATGGCAGCATCACAGTGGCTGCTCGCGATGTTGGTGTGAACCGGAATACCGCATACGGCTGGGCACGTAAAGCAGGGATCCGTGGTCGCGGGATC
CCCAGCACCGGGCCGCACCCGCGGAAGGACGAGTACTTTCAGCTCAGGGCCGGAGGCCTCTCGCGGCGTGAAGCGGTAGCCATCGTCGGAGTCCATGAGC
GCACCGCGCAGGAATGGGACCGCGGCATCAAACACACGGGCCATGTCCGCATCTATCCAGATGGCCGCCGGGTGGACTACAACACCGGTATGACCACACT
CCTCGCGGGTCCTGCGCTACCAGCTCTGGAAGCCCAACTAGATCCCCGCTTCCTCACGCTGAAGGACCGGGAAGTGATCGCTGACCTGCACCGTGCTGGC
CACAGCTTGCGTGCGATCGGCCGAGCGTTGGGCAGGCCGGCCTCGACGATCAAACGCGAGCTCGATGCCCGTTCAGTGGACGGGGCGTACCAGCCGTATG
CCGCTCAGCGTGGCTGGGCAGCATCTCGGCCCCGGCCGAAGAAACCCAAGCTCTGCCTGCCTTCCCGGCTGCGGGACTACGTCCAGGACAAGCTCATGCT
GCGGTGGTCTCCGGAGCAGATCAGTCACACTCTGGTCAAAGAGTTCCACGACGATGAGAGCATGCGAGTGAGTCCCGAGACTATCTATCAGGCCCTCTAC
CTCCAGGCCCGTGGCGGTCTGAAACGGGAGGTCGCAGCCGCGCTGCGCACCGGCCGGACCCGCCGCAAGCCCCACAAACATCCTGATCAACGCACCTCGC
GGTTCGTTGATCACATGGTGATGATCTCCGACCGCCCACCGCAGGTCGAGGACCGGGCCGTGCCCGGGCACTGGGAAGGCGATCTCATCGTGGGCCCCGG
CAGCGCCTCGGCGATCCTCACCTTGGTCGAGCGTTCCACTCGCTACGTTCTGCTGGGGCACCTGCCCGGTGGCCACAGCGCCGAGGAAGTCCGAGATGTG
CTCACAGCGCTGATCACCACGTTGCCCGCACACCTGCGGGGCTCACTGACCTGGGACCAGGGATGCGAGATGGCACGGCACAAACAATTCACGGTCGCGA
CAGGGGTGCCGGTGTACTTCTGCGACCCCCACTCACCATGGCAGCGCGGTAGCAACGAGAACACCAACGGCCTGTTGCGTCAGTACTTCCCCAAGAGCAC
CGACCTTCGGCCTTACAGCCCGCAGGACCTCGAGCACGTCTCACAGGAACTCAACGCACGCCCACGCAAAACGCTCAACTGGGATACCCCAGCCGAGCGT
CTGCGTGATCTACTGACAACCACATAACCATCAGGTGTTGCGAGGACCCCTAGAAACCGCC
TGGGGATGGCAGCATCACAGTGGCTGCTCGCGATGTTGGTGTGAACCGGAATACCGCATACGGCTGGGCACGTAAAGCAGGGATCCGTGGTCGCGGGATC
CCCAGCACCGGGCCGCACCCGCGGAAGGACGAGTACTTTCAGCTCAGGGCCGGAGGCCTCTCGCGGCGTGAAGCGGTAGCCATCGTCGGAGTCCATGAGC
GCACCGCGCAGGAATGGGACCGCGGCATCAAACACACGGGCCATGTCCGCATCTATCCAGATGGCCGCCGGGTGGACTACAACACCGGTATGACCACACT
CCTCGCGGGTCCTGCGCTACCAGCTCTGGAAGCCCAACTAGATCCCCGCTTCCTCACGCTGAAGGACCGGGAAGTGATCGCTGACCTGCACCGTGCTGGC
CACAGCTTGCGTGCGATCGGCCGAGCGTTGGGCAGGCCGGCCTCGACGATCAAACGCGAGCTCGATGCCCGTTCAGTGGACGGGGCGTACCAGCCGTATG
CCGCTCAGCGTGGCTGGGCAGCATCTCGGCCCCGGCCGAAGAAACCCAAGCTCTGCCTGCCTTCCCGGCTGCGGGACTACGTCCAGGACAAGCTCATGCT
GCGGTGGTCTCCGGAGCAGATCAGTCACACTCTGGTCAAAGAGTTCCACGACGATGAGAGCATGCGAGTGAGTCCCGAGACTATCTATCAGGCCCTCTAC
CTCCAGGCCCGTGGCGGTCTGAAACGGGAGGTCGCAGCCGCGCTGCGCACCGGCCGGACCCGCCGCAAGCCCCACAAACATCCTGATCAACGCACCTCGC
GGTTCGTTGATCACATGGTGATGATCTCCGACCGCCCACCGCAGGTCGAGGACCGGGCCGTGCCCGGGCACTGGGAAGGCGATCTCATCGTGGGCCCCGG
CAGCGCCTCGGCGATCCTCACCTTGGTCGAGCGTTCCACTCGCTACGTTCTGCTGGGGCACCTGCCCGGTGGCCACAGCGCCGAGGAAGTCCGAGATGTG
CTCACAGCGCTGATCACCACGTTGCCCGCACACCTGCGGGGCTCACTGACCTGGGACCAGGGATGCGAGATGGCACGGCACAAACAATTCACGGTCGCGA
CAGGGGTGCCGGTGTACTTCTGCGACCCCCACTCACCATGGCAGCGCGGTAGCAACGAGAACACCAACGGCCTGTTGCGTCAGTACTTCCCCAAGAGCAC
CGACCTTCGGCCTTACAGCCCGCAGGACCTCGAGCACGTCTCACAGGAACTCAACGCACGCCCACGCAAAACGCTCAACTGGGATACCCCAGCCGAGCGT
CTGCGTGATCTACTGACAACCACATAACCATCAGGTGTTGCGAGGACCCCTAGAAACCGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1374 bp | 457 aa | 54 | 1427 | + | No |
Chemistry : DDE
ORF sequence :
VLSDELKVAFFQALERGDGSITVAARDVGVNRNTAYGWARKAGIRGRGIPSTGPHPRKDEYFQLRAGGLSRREAVAIVGVHERTAQEWDRGIKHTGHVRI
YPDGRRVDYNTGMTTLLAGPALPALEAQLDPRFLTLKDREVIADLHRAGHSLRAIGRALGRPASTIKRELDARSVDGAYQPYAAQRGWAASRPRPKKPKL
CLPSRLRDYVQDKLMLRWSPEQISHTLVKEFHDDESMRVSPETIYQALYLQARGGLKREVAAALRTGRTRRKPHKHPDQRTSRFVDHMVMISDRPPQVED
RAVPGHWEGDLIVGPGSASAILTLVERSTRYVLLGHLPGGHSAEEVRDVLTALITTLPAHLRGSLTWDQGCEMARHKQFTVATGVPVYFCDPHSPWQRGS
NENTNGLLRQYFPKSTDLRPYSPQDLEHVSQELNARPRKTLNWDTPAERLRDLLTTT
YPDGRRVDYNTGMTTLLAGPALPALEAQLDPRFLTLKDREVIADLHRAGHSLRAIGRALGRPASTIKRELDARSVDGAYQPYAAQRGWAASRPRPKKPKL
CLPSRLRDYVQDKLMLRWSPEQISHTLVKEFHDDESMRVSPETIYQALYLQARGGLKREVAAALRTGRTRRKPHKHPDQRTSRFVDHMVMISDRPPQVED
RAVPGHWEGDLIVGPGSASAILTLVERSTRYVLLGHLPGGHSAEEVRDVLTALITTLPAHLRGSLTWDQGCEMARHKQFTVATGVPVYFCDPHSPWQRGS
NENTNGLLRQYFPKSTDLRPYSPQDLEHVSQELNARPRKTLNWDTPAERLRDLLTTT
Blast result :
Comments
ISKrh4 is 83% aa similar To ISCmi3.
ISKrh4 was found in the chromosome of the laboratory contaminant K. rhizophila Noodlococcus. Two identical copies of ISKrh4 are present in Noodlococcus, each flanked by a 4 bp target site duplication. Additionnal Accession number: CP072262.
Two copies of ISKrh4 are present in the chromosome of K. rhizophila 28R2A-20, which was isolated from the environment associated with an Atlantic Ocean sponge. One of these is identical to the copies in Noodlococcus, and the other differs by three SNPs. Neither copy in 28R2A-20 is flanked by a target site duplication.
ISKrh4 was found in the chromosome of the laboratory contaminant K. rhizophila Noodlococcus. Two identical copies of ISKrh4 are present in Noodlococcus, each flanked by a 4 bp target site duplication. Additionnal Accession number: CP072262.
Two copies of ISKrh4 are present in the chromosome of K. rhizophila 28R2A-20, which was isolated from the environment associated with an Atlantic Ocean sponge. One of these is identical to the copies in Noodlococcus, and the other differs by three SNPs. Neither copy in 28R2A-20 is flanked by a target site duplication.
References
1] Robert Moran (2022) Direct submission.
2] Ho,S.F., McCallum,G.E., Moran,R.A., McInnes,R.S., Wildsmith,A.J., Nicholls,S.M., Quick,J. and van Schaik,W. (2022) Direct GenBank submission.
2] Ho,S.F., McCallum,G.E., Moran,R.A., McInnes,R.S., Wildsmith,A.J., Nicholls,S.M., Quick,J. and van Schaik,W. (2022) Direct GenBank submission.